Molecular Diagnosis of Infectious Diseases / Edition 2

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In Molecular Diagnosis of Infectious Diseases leading international authorities provide a comprehensive and up-to-date set of basic and advanced methods that illuminate the principles of molecular diagnostics and allow researchers to establish assay systems tailored to their specific needs. This unique collection of protocols covers the latest strategies for identifying novel diagnostic markers, epitope mapping, and the production and purification of recombinant antigens, as well as their use as diagnostic reagents in immunological assays. It also includes promising techniques based on recent developments in antibody engineering and on the use of biosensors.. "Molecular Diagnosis of Infectious Diseases offers clinical microbiologists, clinical chemists, and pathologists unique tools with which to develop improved assays for diagnosing infectious diseases.

The book contains black-and-white illustrations.

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Editorial Reviews

Roberta B. Carey
This spiral bound manual contains 36 procedures for the diagnosis of infectious diseases using molecular techniques. The manual describes in "cookbook" form molecular methods to detect microorganisms in patient specimens. The methods are not targeted to detect any particular pathogen. The protocols are universal and may be adapted to many organisms. Collating this variety of protocols into one book is useful as a resource and a teaching manual. The text is written for researchers in the field who have some experience and familiarity with the terms and concepts. It is not for neophytes taking their first steps into molecular diagnosis. The writing is very technical and the theories behind each procedure are briefly described. The contributors comprise an international group of scientists. The black-and-white illustrations clarify the complex concepts, and the photos of the gels illustrate the protocol results. The majority of references were written within the last five years, which would be expected for a book covering a new and emerging science. The text is written in outline form with bold headings. There is no glossary nor are color enhanced diagrams provided, but there is a chapter describing how to use the Internet as a resource for molecular information and interchange. This is the thirteenth book in the Methods in Molecular Medicine series. It is not a book read for general information on the molecular diagnosis of infectious diseases, but one which provides details and disadvantages and usefulness of a particular method. It covers both antigen- and antibody-based methods and highlights critical steps. The manufacturers of the reagents are listed, which directs thereader to the best suppliers with a proven track record of success. This small sized manual contains lots of information for the serious students of molecular techniques.
Compiled by Reischl (U. of Regenburg, Germany), 36 papers by international authorities present standard and cutting-edge methods illustrating the principles of molecular diagnostics, and their applications in diverse immunological assay systems (e.g. diagnostic markers, epitope mapping, and recombinant antigen production). Emphasizing recent developments in antibody engineering such as biosensor technology, this guide addresses basic problems in serological laboratory diagnosis, and includes protocols designed for tailoring test systems to specific needs. Plastic comb binding. Annotation c. by Book News, Inc., Portland, Or.
Doody's Review Service
Reviewer: Richard J Baltaro, MD, PhD (Creighton University School of Medicine)
Description: This inclusive book on the molecular diagnosis of infectious diseases is one in the series, Methods in Molecular Medicine. Its 25 chapters are written by a total of 57 international contributors from Europe and North America.
Purpose: This is a refreshing view highlighting the equal methodological advance of infectious disease sero-diagnosis by recombinant proteins and antibodies as well as proteinomics, rather than the emphasis on different aspects of PCR, as it is done in countless newly published books. This is fundamental in the field of proteinomics.
Audience: Rather than focusing on particular infectious agents, the collection emphasizes general principles and methods and will be especially useful to researchers and students to get familiar with the principles of molecular diagnoses.
Features: The first 10 chapters look at various widely used approaches and methods of identification of antigens and epitopes. The middle section examines in depth expression, purification, modification, renaturation, and characterization of recombinant proteins. The final chapters evaluate recombinant proteins in immunological test systems. Clinicians and medical or laboratory scientists will be challenged, as well as aided, by these final chapters dealing with various basic problems solid support assays and serological diagnosis and an evaluation of molecular diagnostic resources available on the Internet.
Assessment: Each chapter is written in the usual scientific paper format of an abstract, introduction, materials, methods, notes or conclusions and references. The index is well done and makes the book particularly helpful as a reference manual. The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics.

3 Stars from Doody
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Product Details

  • ISBN-13: 9781617374302
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/10/2010
  • Series: Methods in Molecular Medicine Series, #94
  • Edition description: Softcover reprint of hardcover 2nd ed. 2004
  • Edition number: 2
  • Pages: 464
  • Product dimensions: 9.00 (w) x 6.00 (h) x 1.07 (d)

Table of Contents

Part I. Protein-Based Identification of Antigens

Proteomic Approaches to Antigen Discovery
Karen M. Dobos, John S. Spencer, Ian M. Orme, and John T. Belisle

Alexander Krah and Peter R. Jungblut

Immunoprecipitation and Blotting: The Visualization of Small Amounts of Antigens Using Antibodies and Lectins
Stephen Thompson

Part II. DNA-Based Identification of Antigens

Representational Difference Analysis of cDNA
Lucas D. Bowler

Microarray Data Analysis and Mining
Silvia Saviozzi, Giovanni Iazzetti, Enrico Caserta, Alessandro Guffanti, and Raffaele A. Calogero

Expression Cloning
Michael J. Lodes, Davin C. Dillon, Raymond L. Houghton, and Yasir A. W. Skeiky

Part III. Identification of Epitopes and Immunomodulatory Components

Determination of Epitopes by Mass Spectrometry
Christine Hager-Braun and Kenneth B. Tomer

Identification of T Cell Epitopes Using ELISpot and Peptide Pool Arrays
Timothy W. Tobery and Michael J. Caulfield

Virus-like Particles: A Novel Tool for the Induction and Monitoring of Both T-Helper and Cytotoxic T-Lymphocyte Activity
Ludwig Deml, Jens Wild, and Ralf Wagner

Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components
Ludwig Deml, Michael Aigner, Alexander Eckhardt, Jochen Decker, Norbert Lehn, and Wulf Schneider-Brachert

Part IV. Expression of Recombinant Proteins

High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format
Jutta Drees, Jason Smith, Frank Schäfer, and Kerstin Steinert

Production of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant Proteins
Markus Fuhrmann

Codon-Optimized Genes that Enable Increased Heterologous Expression inMammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA
Marcus Graf, Ludwig Deml, and Ralf Wagner

Part V. Purification, Modification, and Renaturation of Recombinant Proteins

Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies)
Udo Reischl

Purification of Recombinant Proteins with High Isoelectric Points
Raffaele A. Calogero and Anna Aulicino

Refolding of Inclusion Body Proteins
Marcus Mayer and Johannes Buchner

Small-Molecule-Protein Conjugation Procedures
Stephen Thompson

Part VI. Characterization of Recombinant Proteins

Structural Characterization of Proteins and Peptides
Rainer Deutzmann

Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors
Claudia Hahnefeld, Stephan Drewianka, and Friedrich W. Herberg

Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor
Uwe Schaible, Michael Liss, Elke Prohaska, Jochen Decker, Karin Stadtherr, and Hans Wolf

Part VII. Evaluation of Recombinant Proteins in Immunological Test Systems

Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays
John E. Butler

Design and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays
Alan Warnes, Anthony R. Fooks, and John R. Stephenson

Basic Problems of Serological Laboratory Diagnosis
Walter Fierz

Molecular Diagnostics Resources on the Internet
Larry Winger

Part VIII. Recombinant Receptor Molecules

Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells
Lars Toleikis, Olaf Broders, and Stefan Dübel


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