Molecular Diagnostic PCR Handbook / Edition 1

Molecular Diagnostic PCR Handbook / Edition 1

by Gerrit J. Viljoen
     
 

ISBN-10: 1402034032

ISBN-13: 9781402034039

Pub. Date: 07/19/2005

Publisher: Springer Netherlands

This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the theoretical aspects related to PCR, setting-up of a PCR laboratory and good Laboratory Practice and standardised PCR protocols to detect animal disease pathogens. Examples of Standard…  See more details below

Overview

This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the theoretical aspects related to PCR, setting-up of a PCR laboratory and good Laboratory Practice and standardised PCR protocols to detect animal disease pathogens. Examples of Standard Operating Procedures as used in individual specialist laboratories and an outline of training materials necessary for PCR technology transfer are presented.

Product Details

ISBN-13:
9781402034039
Publisher:
Springer Netherlands
Publication date:
07/19/2005
Edition description:
2005
Pages:
308
Product dimensions:
6.14(w) x 9.13(h) x 0.03(d)

Table of Contents

Ch. 1Background3
Ch. 2PCR - the basic reaction20
Ch. 3Amplicon detection, analysis and PCR evaluation50
Ch. 4Limitations and troubleshooting60
Ch. 5Specific types of PCR assays74
Ch. 6The PCR laboratory101
Ch. 7Disease diagnosis using PCR specific procedures for important veterinary pathogens120
App. AThermal cyclers203
App. BBasic protocol for agarose gel electrophoresis208
App. CUsing nucleic acid hybridisation in order to verify the integrity of a PCR amplicon210
App. DDigestion of DNA with restriction endonucleases and electrophoresis of the restriction fragments214
App. EDNA sequence analysis217
App. FAn example of a RT-PCR protocol222
App. G (i)Calibration and maintenance of mechanical pipettes225
App. G (ii)Standard operating procedure for using micropipettes230
App. HStandard operating procedure for using clocks and timers232
App. ITheoretical background to pH, buffers and concentration of solutions234
App. JGeneral buffer and stock solutions242
App. KGeneral reagents and suppliers246
App. LBioinformatics250

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