Molecular Diagnostic PCR Handbook / Edition 1

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Overview

This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the theoretical aspects related to PCR, setting-up of a PCR laboratory and good Laboratory Practice and standardised PCR protocols to detect animal disease pathogens. Examples of Standard Operating Procedures as used in individual specialist laboratories and an outline of training materials necessary for PCR technology transfer are presented.
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Product Details

  • ISBN-13: 9781402034039
  • Publisher: Springer Netherlands
  • Publication date: 7/19/2005
  • Edition description: 2005
  • Edition number: 1
  • Pages: 308
  • Product dimensions: 9.21 (w) x 6.14 (h) x 0.75 (d)

Table of Contents

Ch. 1 Background 3
Ch. 2 PCR - the basic reaction 20
Ch. 3 Amplicon detection, analysis and PCR evaluation 50
Ch. 4 Limitations and troubleshooting 60
Ch. 5 Specific types of PCR assays 74
Ch. 6 The PCR laboratory 101
Ch. 7 Disease diagnosis using PCR specific procedures for important veterinary pathogens 120
App. A Thermal cyclers 203
App. B Basic protocol for agarose gel electrophoresis 208
App. C Using nucleic acid hybridisation in order to verify the integrity of a PCR amplicon 210
App. D Digestion of DNA with restriction endonucleases and electrophoresis of the restriction fragments 214
App. E DNA sequence analysis 217
App. F An example of a RT-PCR protocol 222
App. G (i) Calibration and maintenance of mechanical pipettes 225
App. G (ii) Standard operating procedure for using micropipettes 230
App. H Standard operating procedure for using clocks and timers 232
App. I Theoretical background to pH, buffers and concentration of solutions 234
App. J General buffer and stock solutions 242
App. K General reagents and suppliers 246
App. L Bioinformatics 250
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