Molecular Diagnostics / Edition 1

Molecular Diagnostics / Edition 1

by William B. Coleman
     
 

ISBN-10: 0896033732

ISBN-13: 9780896033733

Pub. Date: 12/20/2009

Publisher: Springer-Verlag New York, LLC

This updated and expanded tutorial guide to molecular diagnostic techniques takes advantage of many new molecular technologies to include both improved traditional methods and totally new methods, some not yet in routine use. The authors offer cutting-edge molecular diagnostics for genetic disease, human cancers, infectious diseases, and identity testing, as well as…  See more details below

Overview

This updated and expanded tutorial guide to molecular diagnostic techniques takes advantage of many new molecular technologies to include both improved traditional methods and totally new methods, some not yet in routine use. The authors offer cutting-edge molecular diagnostics for genetic disease, human cancers, infectious diseases, and identity testing, as well as new insights into the question of quality assurance in the molecular diagnostics laboratory. Additional chapters address other technologies found in the clinical laboratory that complementary to molecular diagnostic technologies and discuss genetic counseling and the ethical and social issues involved with nucleic acid testing. A value-added compact disk containing a companion e-book version of the book, is included for downloading and use in the reader's PC or PDA.

Product Details

ISBN-13:
9780896033733
Publisher:
Springer-Verlag New York, LLC
Publication date:
12/20/2009
Series:
Pathology and Laboratory Medicine Series
Edition description:
Older Edition
Pages:
404
Product dimensions:
0.94(w) x 10.00(h) x 7.00(d)

Table of Contents

1An historical perspective on the clinical diagnostic laboratory3
2An overview of nucleic acid biochemistry, structure, and function : the foundations of molecular biology13
3Extraction of nucleic acids25
4Nucleic Acid blotting techniques : theory and practice31
5The polymerase chain reaction47
6Bioinformatics : computer-based approaches to genetic analysis57
7PCR-based methods for mutation detection65
8Alternative methods for amplified nucleic acid testing75
9Electrophoretic methods for mutation detection and DNA sequencing85
10Single nucleotide polymorphisms : testing DNA variation for disease association111
11Microarray approaches to gene expression analysis121
12Methods for analysis of DNA methylation149
13Flow Cytometry163
14Medical cytogenetics173
15Fluorescence in situ hybridization : a major milestone in luminous cytogenetics189
16Immunohistochemistry203
17Laser capture microdissection219
18Framework for quality assurance principles227
19Verification of molecular assays237
20Standards and standardization of molecular diagnostics243
21Laboratory-developed assays in molecular diagnostics247
22An overview of molecular genetics259
23Genetic basis of neurologic and neuromuscular diseases267
24Molecular mechanisms of endocrine disorders281
25Molecular pathogenesis of cardiovascular disease295
26Molecular diagnostics in coagulation311
27Cystic fibrosis321
28Prenatal genotyping for identification of fetuses at risk for immune cytopenic disorders329
29Personalized medicine341
30Molecular pathogenesis of human cancer349
31Application of molecular diagnostics to hereditary nonpolyposis colorectal cancer375
32Molecular genetic applications to the diagnosis of lymphoma393
33Molecular genetic abnormalities in acute and chronic leukemias415
34Molecular testing for Chlamydia trachomatis and Neisseria gonorrhoeae437
35Human papillomavirus447
36Molecular diagnosis for HIV-1453
37Hepatitis C461
38Cytomegalovirus473
39HLA Typing using molecular methods485
40Molecular analysis for forensic casework and parentage testing495
41Molecular assessment of bone marrow transplant engraftment511
42The use of DNA-based identity testing for specimen identification517
43Genetic counseling considerations in molecular diagnosis525
44Ethical, social, and legal issues related to molecular genetic testing545

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