Molecular Genetics of Bacteria / Edition 5

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Overview

The Fourth Edition of this highly successful book provides an essential introduction to the molecular genetics of bacteria. Thoroughly revised and updated, Molecular Genetics of Bacteria now includes a much greater coverage of genomics, microarrays and proteomics. The text includes an enhanced treatment of the ways in which both classical and modern genetics have contributed to our understanding of how bacteria work. The focus of the book remains firmly on bacteria and will be invaluable to those students studying microbiology, biotechnology, molecular biology, biochemistry, genetics and related biomedical sciences.

"...thoroughly updated with new coverage of such topics as the concept of adaptive mutation,bacterial differentiation, intercellular signalling,conjugative transposons,and integ- rons...enhanced coverage of supercoining, PCR, and more."

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Editorial Reviews

Booknews
The coverage is restricted to bacterial genetics to provide a less daunting introduction for students who don't intend to pursue a career in genetics, but the material assumes a working knowledge of molecular biology. Details essential principles of nucleic acid structure and function, methods and terminology, regulation of gene expression, and extrachromosomal inheritance, and discusses recent developments in applied in vivo and in vitro genetics. Includes b&w diagrams, a glossary, and descriptions of enzymes and genes. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Doody's Review Service
Reviewer: Rebecca T. Horvat, PhD, D (ABMM)(University of Kansas Medical Center)
Description: This textbook teaches upper-level bacterial genetics at the college and graduate level, starting with the basics of molecular structure and building to the mapping of bacterial genomes..
Purpose: The purpose of this sixth edition is to provide an update of the field. In the six years since the previous edition, there has been an extraordinary increase in both the information on bacterial genetics and the techniques used to study them.
Audience: Higher-level students studying bacterial genetics are the intended audience.
Features: The book starts with basic principles of DNA structure, including the various forms DNA takes. It then presents the basic information on hybridization and transcription as well as translation. The explanation of gene organization includes how start and stop codons influence expression and how changes can influence tertiary structure. This is the foundation for the rest of the book, which covers such topics as genetic evolution that relies on variability in the genetic replication, the mechanisms that allow mutations to occur, and how bacterial genes are transferred to other bacterial strains. Later chapters cover new developments and techniques that have contributed to new information such as expression plasmids and specific mutagenesis to examine function. Additional chapters discuss comparative genomics and the analysis of gene expression. The book also demonstrates the relationship of growth in the knowledge of bacterial genomics to other fields such as biotechnology and bioengineering.
Assessment: This update of a popular textbook provides the latest information and techniques used to study bacterial genetics. It provides descriptive information, experimental methods, and clear descriptions of bacterial molecular genetics.
From the Publisher
"This update of a popular textbook provides the latest information and techniques used to study bacterial genetics. It provides descriptive information, experimental methods, and clear descriptions of bacterial molecular genetics." (Doody's, 19 August 2011)
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Product Details

  • ISBN-13: 9780470741849
  • Publisher: Wiley
  • Publication date: 6/8/2010
  • Edition description: New Edition
  • Edition number: 5
  • Pages: 400
  • Product dimensions: 6.60 (w) x 9.50 (h) x 0.60 (d)

Table of Contents

Preface.

1 Nucleic Acid Structure and Function.

1.1 Structure of nucleic acids.

1.1.1 DNA.

1.1.2 RNA.

1.1.3 Hydrophobic interactions.

1.1.4 Different forms of the double helix.

1.1.5 Supercoiling.

1.1.6 Denaturation and hybridization.

1.1.7 Orientation of nucleic acid strands.

1.2 Replication of DNA.

1.2.1 Unwinding and rewinding.

1.2.2 Fidelity of replication; proofreading.

1.3 Chromosome replication and cell division.

1.4 DNA repair.

1.4.1 Mismatch repair.

1.4.2 Excision repair.

1.4.3 Recombination (post-replication) repair.

1.4.4 SOS repair.

1.5 Gene expression.

1.5.1 Transcription.

1.5.2 Translation.

1.5.3 Post-translational events.

1.6 Gene organization.

2 Mutation and Variation.

2.1 Variation and evolution.

2.1.1 Fluctuation test.

2.1.2 Replica plating.

2.1.3 Directed mutation in bacteria?

2.2 Types of mutation.

2.2.1 Point mutations.

2.2.2 Conditional mutants.

2.2.3 Variation due to larger-scale DNA alterations.

2.2.4 Extrachromosomal agents and horizontal gene transfer.

2.3 Recombination.

2.3.1 A model of the general (homologous) recombination process.

2.3.2 Enzymes involved in recombination.

2.4 Phenotypes.

2.4.1 Restoration of phenotype.

2.5 Mechanisms of mutation.

2.5.1 Spontaneous mutation.

2.5.2 Chemical mutagens.

2.5.3 Ultraviolet irradiation.

2.6 Isolation and identification of mutants.

2.6.1 Mutation and selection.

2.6.2 Replica plating.

2.6.3 Isolation of other mutants.

2.6.4 Molecular methods.

3 Regulation of Gene Expression.

3.1 Gene copy number.

3.2 Transcriptional control.

3.2.1 Promoters.

3.2.2 Terminators, attenuators and anti-terminators.

3.2.3 Induction and repression: regulatory proteins.

3.2.4 Two-component regulatory systems.

3.2.5 Global regulatory systems.

3.2.6 Quorum sensing.

3.3 Translational control.

3.3.1 Ribosome binding.

3.3.2 Codon usage.

3.3.3 Stringent response.

3.3.4 Regulatory RNA.

3.4 Phase variation.

4 Genetics of Bacteriophages.

4.1 Bacteriophage structure.

4.2 Single-strand DNA bacteriophages.

4.2.1 ϕX174.

4.2.2 M13.

4.3 RNA-containing phages: MS2.

4.4 Double-stranded DNA phages.

4.4.1 Bacteriophage T4.

4.4.2 Bacteriophage λ.

4.4.3 Lytic and lysogenic regulation of bacteriophage λ.

4.5 Restriction and modification.

4.6 Bacterial resistance to phage attack.

4.7 Complementation and recombination.

4.8 Why are bacteriophages important?

4.8.1 Phage typing.

4.8.2 Phage therapy.

4.8.3 Phage display.

4.8.4 Phages in the natural environment.

4.8.5 Bacterial virulence and phage conversion.

5 Plasmids.

5.1 Some bacterial characteristics are determined by plasmids.

5.1.1 Antibiotic resistance.

5.1.2 Colicins and bacteriocins.

5.1.3 Virulence determinants.

5.1.4 Plasmids in plant-associated bacteria.

5.1.5 Metabolic activities.

5.2 Molecular properties of plasmids.

5.2.1 Plasmid replication and control.

5.2.2 Partitioning.

5.2.3 Host range.

5.2.4 Plasmid incompatibility.

5.3 Plasmid stability.

5.3.1 Plasmid integrity.

5.3.2 Partitioning.

5.3.3 Differential growth rate.

5.4 Associating a plasmid with a phenotype.

6 Gene Transfer.

6.1 Transformation.

6.2 Conjugation.

6.2.1 Mechanism of conjugation.

6.2.2 The F plasmid.

6.2.3 Conjugation in other bacteria.

6.3 Transduction.

6.3.1 Specialized transduction.

6.4 Recombination.

6.4.1 Consequences of recombination.

6.4.2 Site-specific and non-homologous (illegitimate) recombination.

6.5 Mosaic genes and chromosome plasticity.

7 Genomic Plasticity: Movable Genes and Phase Variation.

7.1 Insertion sequences.

7.1.1 Structure of insertion sequences.

7.1.2 Occurrence of insertion sequences.

7.2 Transposons.

7.2.1 Structure of transposons.

7.2.2 Integrons.

7.2.3 ISCR elements.

7.3 Mechanisms of transposition.

7.3.1 Replicative transposition.

7.3.2 Non-replicative (conservative) transposition.

7.3.3 Regulation of transposition.

7.3.4 Activation of genes by transposable elements.

7.3.5 Mu: A transposable bacteriophage.

7.3.6 Conjugative transposons.

7.4 Phase variation.

7.4.1 Variation mediated by simple DNA inversion.

7.4.2 Variation mediated by nested DNA inversion.

7.4.3 Antigenic variation in the gonococcus.

7.4.4 Phase variation by slipped-strand mispairing.

7.4.5 Phase variation mediated by differential DNA methylation.

7.5 Clustered regularly interspersed short palindromic repeats.

8 Genetic Modification: Exploiting the Potential of Bacteria.

8.1 Strain development.

8.1.1 Generation of variation.

8.1.2 Selection of desired variants.

8.2 Overproduction of primary metabolites.

8.2.1 Simple pathways.

8.2.2 Branched pathways.

8.3 Overproduction of secondary metabolites.

8.4 Gene cloning.

8.4.1 Cutting and joining DNA.

8.4.2 Plasmid vectors.

8.4.3 Bacteriophage λ vectors.

8.4.4 Cloning larger fragments.

8.4.5 Bacteriophage M13 vectors.

8.5 Gene libraries.

8.5.1 Construction of genomic libraries.

8.5.2 Screening a gene library.

8.5.3 Cloning PCR products.

8.5.4 Construction of a cDNA library.

8.6 Products from cloned genes.

8.6.1 Expression vectors.

8.6.2 Making new genes.

8.6.3 Other bacterial hosts.

8.6.4 Novel vaccines.

8.7 Other uses of gene technology.

9 Genetic Methods for Investigating Bacteria.

9.1 Metabolic pathways.

9.1.1 Complementation.

9.1.2 Cross-feeding.

9.2 Microbial physiology.

9.2.1 Reporter genes.

9.2.2 Chromatin immunoprecipitation.

9.2.3 Cell division.

9.2.4 Motility and chemotaxis.

9.2.5 Cell differentiation.

9.3 Bacterial virulence.

9.3.1 Wide-range mechanisms of bacterial pathogenesis.

9.3.2 Detection of virulence genes.

9.4 Specific mutagenesis.

9.4.1 Gene replacement.

9.4.2 Antisense RNA.

9.5 Taxonomy, evolution and epidemiology.

9.5.1 Molecular taxonomy.

9.5.2 GC content.

9.5.3 16 S rRNA.

9.5.4 Denaturing-gradient gel electrophoresis and temperature-gradient gel electrophoresis.

9.5.5 Diagnostic use of PCR.

9.5.6 Molecular epidemiology.

10 Gene Mapping to Genomics and Beyond.

10.1 Gene mapping.

10.1.1 Conjugational analysis.

10.1.2 Gene libraries.

10.1.3 Restriction mapping and pulsed-field gel electrophoresis.

10.2 DNA sequence determination.

10.2.1 Sanger sequencing.

10.2.2 Dye terminator sequencing.

10.2.3 Pyrosequencing.

10.2.4 Massively parallel sequencing.

10.3 Genome sequencing.

10.3.1 Genome-sequencing strategies.

10.3.2 Relating sequence to function.

10.3.3 Metagenomics.

10.4 Comparative genomics.

10.4.1 Microarrays.

10.5 Analysis of gene expression.

10.5.1 Transcriptional analysis.

10.5.2 Translational analysis.

10.6 Metabolomics.

10.7 Systems biology and synthetic genomics.

10.7.1 Systems biology.

10.7.2 Synthetic genomics.

10.8 Conclusion.

Appendix A Further Reading.

Appendix B Abbreviations Used.

Appendix C Glossary.

Appendix D Enzymes and other Proteins.

Appendix E Genes.

Appendix F Standard Genetic Code.

Appendix G Bacterial Species.

Index.

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