Molecular Genetics of Yeast: A Practical Approach

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The book covers comprehensively all current experimental procedures used in the research of the genetics and molecular biology of the yeast Saccharomyces cerevisiae. Featuring detailed protocols and practical tips, it guarantees easy access to a wide range of specialized topics within this rapidly advancing field. Internationally-recognized experts present all methods currently in use, discussing topics such as DNA isolulation, cloning and expression vectors, cosmid cloning, construction and use of cDNA libraries, plasmid shuffling and mutant isolulation. Chapters on Ty insertional mutagenesis, high efficiency transformation, cell-free translation of mRNAs, Ty virus-like particles, and applications to industrial strains of yeast are also included. Researchers in the fields of molecular biology, genetics, and biochemistry working with this yeast, as well as professionals of the biotechnology industry will refer to this practical reference frequently.

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Editorial Reviews

A detailed compendium of protocols and practical tips for researchers working with the yeast Saccharomyces cerevisiae, which is both an important industrial organism and a favorite subject for research into molecular biology, genetics, and biotechnology. The procedures described include DNA isolation, cloning and expression vectors, Ty insertional mutagenesis, and virus-like particles. Annotation c. Book News, Inc., Portland, OR (
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Product Details

  • ISBN-13: 9780199634293
  • Publisher: Oxford University Press, USA
  • Publication date: 2/28/2002
  • Series: Practical Approach Series, #141
  • Edition description: New Edition
  • Pages: 312
  • Product dimensions: 6.19 (w) x 9.25 (h) x 0.68 (d)

Table of Contents

Procedures for isolating yeast DNA for different purposes Construction of cloning and expression vectors Cosmid cloning of yeast DNA The construction and use of cDNA libraries for genetic selections Pulsed field gel electrophoresis Plasmid shuffling and mutant isolation Ty insertional mutagenesis High efficiency transformation with lithium acetate Measurement of transcription Measurement of mRNA stability Production of foreign proteins at high level Cell-free translation of natural and synthetic mRNAs Virus-like particles: Ty retrotransposons The K1 killer toxin: molecular and genetic applications to secretion and cell surface assembly Immuno-electron microscopy Industrial Saccharomyces yeasts

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