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Molecular Microbiology Laboratory, Second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles.
Audience: The market for this book is quite large. It is appropriate for college sophomores through seniors in any of the life sciences, including microbiology, genetics, general biology, biochemistry, molecular biology, botany, zoology, etc. The experiments are also appropriate for properly equipped junior colleges and liberal arts institutions.
Introduction Experiment 1: Plasmid Purification and Restriction Mapping Experiment 2: Affinity Purification of Histidine-Tagged FnbA Protein Experiment 3: Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes Experiment 4: Terminal Restriction Fragment Length Polymorphism Analysis of 16 S Ribosomal RNA Genes from Uncultured Bacterial Communities Appendix A: Sample Laboratory Reports Appendix B: Sample Proposals Appendix C: Sample Tests Appendix D: Guidance for Presentations Appendix E: Revised Paragraph from J. Biol. Chem. 266: 3811-3814 (1991) Appendix F: Abstract from Appl. Environ. Microbiol. 63: 2647-2653 (1997) Appendix G: Sequence Editing Tutorial Appendix H: Peak Scanner Tutorial Appendix I: Adding Size Standards to Peak Scanner Appendix J: PRIMER Tutorial Appendix K: Checklist for Grading Laboratory Reports Appendix L: Peer Review Checklist Appendix M: Career Advice Appendix N: Molecular Microbiology Laboratory Preparation Manual Bibliography Index