Molecular Systematics / Edition 2

Molecular Systematics / Edition 2

by David M. Hillis
     
 

ISBN-10: 0878932828

ISBN-13: 9780878932825

Pub. Date: 01/28/1996

Publisher: Sinauer Associates, Incorporated

Molecular systematic methods have been applied in most fields of biology to provide an evolutionary framework whenever comparisons are made among individuals, populations, or higher taxa. The first edition of Molecular Systematics became a standard reference for this vigorous field by describing each aspect of the planning, execution, and analysis of a molecular

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Overview

Molecular systematic methods have been applied in most fields of biology to provide an evolutionary framework whenever comparisons are made among individuals, populations, or higher taxa. The first edition of Molecular Systematics became a standard reference for this vigorous field by describing each aspect of the planning, execution, and analysis of a molecular systematic study. The Second Edition updates and expands this coverage, and includes considerable information on new molecular techniques and methods of analysis. Molecular Systematics includes chapters on sampling design, the collection and storage of tissues, each of the major molecular techniques, and intraspecific and phylogenetic analysis. The sampling chapters describe how to plan a study and how to collect, transport, and store the appropriate tissues for each study. The techniques chapters cover principles, assumptions, applications, limitations, and protocols for isozyme electrophoresis, molecular cytogenetics, DNA hybridization, the polymerase chain reaction, restriction site and fragment analysis, and nucleic acid sequencing and alignment. Advantages and disadvantages of alternative approaches are discussed for each technique, and recent developments (such as new methods of fluorescent in situ hybridization, rapid screening methods for detecting DNA sequence variation, automated sequencing methods, new approaches for PCR, and microsatellite analyses) are detailed. Three additional chapters cover the rationale and methodology of molecular data analysis at both the population and interspecific levels, and provide information on using and obtaining the relevant computer programs (including the many programs available for free across the Internet). The chapter on phylogenetic analysis has been considerably expanded to include the latest developments in maximum likelihood analysis, spectral analysis, methods for reconstructing reticulating networks, corrections for compl

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Product Details

ISBN-13:
9780878932825
Publisher:
Sinauer Associates, Incorporated
Publication date:
01/28/1996
Edition description:
REV
Pages:
655
Product dimensions:
7.87(w) x 9.84(h) x (d)

Table of Contents

1Molecular Systematics: Context and Controversies1
2Project Design17
3Collection and Storage of Tissues29
4Proteins: Isozyme Electrophoresis51
5Chromosomes: Molecular Cytogenetics121
6Nucleic Acids I: DNA-DNA Hybridization169
7Nucleic Acids II: The Polymerase Chain Reaction205
8Nucleic Acids III: Analysis of Fragments and Restriction Sites249
9Nucleic Acids IV: Sequencing and Cloning321
10Intraspecific Differentiation385
11Phylogenetic Inference407
12Applications of Molecular Systematics: The State of the Field and a Look to the Future515

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