Molecular Systematics / Edition 2

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1999 Paperback NEW 9780878932825 Paperback, 655pp. This listing is a new book, a title currently in-print which we order directly and immediately from the publisher. ... *****PLEASE NOTE: This item is shipping from an authorized seller in Europe. In the event that a return is necessary, you will be able to return your item within the US. To learn more about our European sellers and policies see the BookQuest FAQ section***** Read more Show Less

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Molecular systematic methods have been applied in most fields of biology to provide an evolutionary framework whenever comparisons are made among individuals, populations, or higher taxa. The first edition of Molecular Systematics became a standard reference for this vigorous field by describing each aspect of the planning, execution, and analysis of a molecular systematic study. The Second Edition updates and expands this coverage, and includes considerable information on new molecular techniques and methods of analysis. Molecular Systematics includes chapters on sampling design, the collection and storage of tissues, each of the major molecular techniques, and intraspecific and phylogenetic analysis. The sampling chapters describe how to plan a study and how to collect, transport, and store the appropriate tissues for each study. The techniques chapters cover principles, assumptions, applications, limitations, and protocols for isozyme electrophoresis, molecular cytogenetics, DNA hybridization, the polymerase chain reaction, restriction site and fragment analysis, and nucleic acid sequencing and alignment. Advantages and disadvantages of alternative approaches are discussed for each technique, and recent developments (such as new methods of fluorescent in situ hybridization, rapid screening methods for detecting DNA sequence variation, automated sequencing methods, new approaches for PCR, and microsatellite analyses) are detailed. Three additional chapters cover the rationale and methodology of molecular data analysis at both the population and interspecific levels, and provide information on using and obtaining the relevant computer programs (including the many programs available for free across the Internet). The chapter on phylogenetic analysis has been considerably expanded to include the latest developments in maximum likelihood analysis, spectral analysis, methods for reconstructing reticulating networks, corrections for compl

"...a standard reference that describes each aspect of the planning, execution, and analysis of a molecular systematic study."

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Editorial Reviews

An introduction and reference to using molecular systematic methods of establishing evolutionary relationships between organisms or groups of organisms. Explains how to design a research project and the protocols for collecting and storing tissues; the techniques for analyzing and comparing proteins, chromosomes, and nucleic acids; and analyzing the results with intraspecific differentiation, and phylogenetic inference. Also suggests future applications. Extensively revised from the 1990 edition; the chapter on immunological methods has been dropped in favor of one on polymerase chain reaction. Includes a glossary without pronunciation. Annotation c. Book News, Inc., Portland, OR (
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Product Details

  • ISBN-13: 9780878932825
  • Publisher: Sinauer Associates, Incorporated
  • Publication date: 1/28/1996
  • Edition description: REV
  • Edition number: 2
  • Pages: 655

Table of Contents

1 Molecular Systematics: Context and Controversies 1
2 Project Design 17
3 Collection and Storage of Tissues 29
4 Proteins: Isozyme Electrophoresis 51
5 Chromosomes: Molecular Cytogenetics 121
6 Nucleic Acids I: DNA-DNA Hybridization 169
7 Nucleic Acids II: The Polymerase Chain Reaction 205
8 Nucleic Acids III: Analysis of Fragments and Restriction Sites 249
9 Nucleic Acids IV: Sequencing and Cloning 321
10 Intraspecific Differentiation 385
11 Phylogenetic Inference 407
12 Applications of Molecular Systematics: The State of the Field and a Look to the Future 515
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