Molecular Zoology: Advances, Strategies and Protocols

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Contemporary tools of molecular biology continue to open new areas of biological research and to provide important answers to classic problems. Zoological questions of mating strategies, physiological adaptation, genetic exchange between populations, cell lineages during development, and many others are now being powerfully addressed using tools from the molecular arsenal. To provide broad access to these tools requires an authoritative reference that highlights recent advances, lays out future strategies, and ...
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1996-05-31 Hardcover Very Good Expedited shipping recommended. Clean copy. No marks or writing in it. Every purchase will benefit global literacy.

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Overview

Contemporary tools of molecular biology continue to open new areas of biological research and to provide important answers to classic problems. Zoological questions of mating strategies, physiological adaptation, genetic exchange between populations, cell lineages during development, and many others are now being powerfully addressed using tools from the molecular arsenal. To provide broad access to these tools requires an authoritative reference that highlights recent advances, lays out future strategies, and provides working protocols to a wide audience of zoological scientists. Molecular Zoology: Advances, Strategies, and Protocols outlines the core concepts of these critical molecular techniques and provides specific instructions for their use. The book is divided into two main parts: Research Strategies and Protocols. The first section features detailed descriptions of the research approaches that incorporate molecular tools in the study of developmental, physiological, ecological, and evolutionary processes. In addition to charting recent advances, this section shows how to interpret results and describes the advantages and disadvantages of alternative approaches. These chapters function as entry points to molecular zoology for broadly trained zoologists without formal molecular training, graduate students, and molecular biologists in other fields. The second section is a compilation of over 60 protocols which have been developed, tested, and perfected by leading researchers in the field. It provides step-by-step coverage of each protocol, featuring for each a summary of its underlying rationale, a list of necessary reagents and solutions, and a discussion of potential obstacles to a particular technique. Molecular Zoology: Advances, Strategies, and Protocols is an authoritative resource designed to provide both basic and in-depth explanations of molecular investigation procedures for research scientists in all areas of organismal and integrative biology,
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Editorial Reviews

Booknews
Presents papers from the January 1995 symposium, highlighting core concepts of contemporary tools in molecular biology and providing specific instructions for their use. Details research approaches that incorporate molecular tools in the study of developmental, physiological, ecological, and evolutionary processes, and offers 60 protocols with step-by-step instructions, lists of reagents and solutions, and discussion of potential obstacles. Techniques covered include PCR-based cloning and regulatory element identification. For researchers and students in zoology, marine biology, and ecology. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9780471144496
  • Publisher: Wiley, John & Sons, Incorporated
  • Publication date: 5/24/1996
  • Edition number: 1
  • Pages: 580
  • Product dimensions: 6.42 (w) x 9.52 (h) x 1.33 (d)

Table of Contents

Foreword
Preface
Protocols
1 Biological Databases on the Internet 3
2 Parametric Bootstrapping in Molecular Phylogenetics: Applications and Performance 19
3 Molecular Approaches and the Growth of Phylogenetic Biology 47
4 Characterization of Genetic Structure and Genealogies Using RAPD-PCR Markers: A Random Primer for the Novice and Nervous 67
5 Macrospatial Genetic Structure and Speciation in Marine Taxa with High Dispersal Abilities 101
6 Molecular Approaches to the Study of Marine Biological Invasions 119
7 Application of Molecular Methods to the Assessment of Genetic Mating Systems in Vertebrates 133
8 Strategies for Finding and Using Highly Polymorphic DNA Microsatellite Loci for Studies of Genetic Relatedness and Pedigrees 163
9 PCR-Based Cloning Across Large Taxonomic Distances and Polymorphism Detection: MHC as a Case Study 181
10 Strategies for Cloning Developmental Genes Using Closely Related Species 197
11 Using Nematode Vulva Development to Model the Evolution of Developmental Systems 209
12 The Analysis of Lineage-Specific Gene Activity During Sea Urchin Development 221
13 Evolutionary Approaches to Analyzing Development 245
14 Revealing Homologies Between Body Parts of Distantly Related Animals by in Situ Hybridization to Developmental Genes: Amphioxus versus Vertebrates 267
15 Evolutionary Transposition and the Vertebrate Hox Genes: Comparing Morphology to Gene Expression Boundaries with in Situ Hybridization 283
16 Lineage Analysis Using Retroviral Vectors 297
17 Osmoregulatory Gene Expression and Implications for Evolutionary Studies: Strategies in Identification of the Osmotic Response Element (ORE) 313
18 A Molecular Approach to the Selectionist/Neutralist Controversy 327
19 Molecular Studies of the Sequential Expression of a Respiratory Protein During Crustacean Development 353
20 The Role of P450[subscript arom] in Sex Determination of Prototheria and Nonmammalian Vertebrates 369
21 Molecular Approaches to Control of Reproductive Behavior and Sexual Differentiation of Brain in Rodents 387
22 Transgenic Fish: Ideal Models for Basic Research and Biotechnological Applications
Protocols 435
Protocols by Author 557
Protocols by Application Codes 561
Index 567
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