Molecular Zoology: Advances, Strategies and Protocols

Molecular Zoology: Advances, Strategies and Protocols

by Joan D. Ferraris
     
 

Molecular Zoology Advances, Strategies, and Protocols

Edited by Joan D. Ferraris and Stephen R. Palumbi

Contemporary tools of molecular biology continue to open new areas of biological research and to provide important answers to classic problems. Zoological questions of mating strategies, physiological adaptation, genetic exchange between populations, cell

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Overview

Molecular Zoology Advances, Strategies, and Protocols

Edited by Joan D. Ferraris and Stephen R. Palumbi

Contemporary tools of molecular biology continue to open new areas of biological research and to provide important answers to classic problems. Zoological questions of mating strategies, physiological adaptation, genetic exchange between populations, cell lineages during development, and many others are now being powerfully addressed using tools from the molecular arsenal. To provide broad access to these tools requires an authoritative reference that highlights recent advances, lays out future strategies, and provides working protocols to a wide audience of zoological scientists.

Molecular Zoology: Advances, Strategies, and Protocols outlines the core concepts of these critical molecular techniques and provides specific instructions for their use. The book is divided into two main parts: Research Strategies and Protocols. The first section features detailed descriptions of the research approaches that incorporate molecular tools in the study of developmental, physiological, ecological, and evolutionary processes. In addition to charting recent advances, this section shows how to interpret results and describes the advantages and disadvantages of alternative approaches. These chapters function as entry points to molecular zoology for broadly trained zoologists without formal molecular training, graduate students, and molecular biologists in other fields. The second section is a compilation of over 60 protocols which have been developed, tested, and perfected by leading researchers in the field. It provides step-by-step coverage of each protocol, featuring for each a summary of its underlying rationale, a list of necessary reagents and solutions, and a discussion of potential obstacles to a particular technique.

Specific techniques covered in the book include:

  • Applications of parametric bootstrapping in molecular phylogenetics
  • Microsatellite analysis of genetic mating systems and genetic relatedness
  • Use of RAPD-PCR markers in genetic structure and genealogies
  • PCR-based cloning across large taxonomic distances
  • Cell lineage analysis using retroviral vectors
  • Osmoregulatory gene characterization and expression
  • Regulatory element identification and transcription factor analysis
  • Protocols for in situ hybridization, DNA footprinting, gene knockout, ribonuclease protection assay, and coupled transcription/translation reactions.

Molecular Zoology: Advances, Strategies, and Protocols is an authoritative resource designed to provide both basic and in-depth explanations of molecular investigation procedures for research scientists in all areas of organismal and integrative biology, including zoology, marine biology, and ecology. With its extensive coverage of molecular protocols, graduate students in biology will also find this book to be an indispensable manual for laboratory work.

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Editorial Reviews

Booknews
Presents papers from the January 1995 symposium, highlighting core concepts of contemporary tools in molecular biology and providing specific instructions for their use. Details research approaches that incorporate molecular tools in the study of developmental, physiological, ecological, and evolutionary processes, and offers 60 protocols with step-by-step instructions, lists of reagents and solutions, and discussion of potential obstacles. Techniques covered include PCR-based cloning and regulatory element identification. For researchers and students in zoology, marine biology, and ecology. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Product Details

ISBN-13:
9780471144618
Publisher:
Wiley
Publication date:
05/28/1996
Pages:
580
Product dimensions:
6.10(w) x 9.21(h) x 1.06(d)

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