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Monoclonal Antibody Protocols / Edition 1
     

Monoclonal Antibody Protocols / Edition 1

by William C. Davis, W. C. Davis
 

ISBN-10: 0896033082

ISBN-13: 9780896033085

Pub. Date: 05/23/1995

Publisher: Springer-Verlag New York, LLC

Monoclonal Antibody Prools provides researchers in biomedical, agricultural, and biological science with a set of detailed, easy-to-follow methods for developing and using monoclonal antibodies. The prools emphasize techniques that optimize the outgrowth of hybridomas from primary cultures of fused cells and the use of an alternative, electric-field-mediated cell

Overview

Monoclonal Antibody Prools provides researchers in biomedical, agricultural, and biological science with a set of detailed, easy-to-follow methods for developing and using monoclonal antibodies. The prools emphasize techniques that optimize the outgrowth of hybridomas from primary cultures of fused cells and the use of an alternative, electric-field-mediated cell fusion technique to increase the yield of hybridomas. The book stresses antibodies produced in mice, but includes methods of producing xenogeneic hybrids that yield human, bovine, equine, and porcine monoclonal antibodies.

With its detailed instructions, its comments on how to alter the various steps of a prool in order to accommodate different materials, and its hints and tips that often make the difference between success and failure, Monoclonal Antibody Prools provides you with a ready and indispensable source of information for preparing and using monoclonal antibodies successfully in your laboratory.

Product Details

ISBN-13:
9780896033085
Publisher:
Springer-Verlag New York, LLC
Publication date:
05/23/1995
Series:
Methods in Molecular Biology Series , #45
Edition description:
1995
Pages:
274
Product dimensions:
6.10(w) x 9.25(h) x 0.03(d)

Table of Contents

Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies. Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro. Culture Conditions that Optimize Outgrowth of Hybridomas. Production of Stable Heterohybridomas Producing Human Monoclonal Antibodies. Production of Stable Bovine-Murine Interspecies Hybrids. Production of Monoclonal Antibodies in Swine. Production of Monoclonal Antibodies in Horses. Use of Electric-Field-Mediated Cell Fusion to Produce Hybridomas Secreting Monoclonal Antibodies. Rapid Isotyping of Mouse Monoclonal Antibodies. Methods of Measuring Antibodies in Sera/Plasma, Ascites, and Tissue-Culture Media. Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay. Use of Monoclonal Antibodies in Immunohishemistry. Use of Monoclonal Antibodies in Immunoelectron Microscopy. Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection. The Use of Directly and Indirectly Labeled Monoclonal Antibodies in Flow Cytometry. Use of Monoclonal Antibodies and Flow Cytometry to Cluster and Analyze Leukocyte Differentiation Molecules. In Vivo and In Vitro Production of Monoclonal Antibodies: Bioreactors vs Immune Ascites. Separation of Monoclonal Antibodies from Cell-Culture Supernatants and Ascites Fluid Using Thiophilic Agarose. Detection, Purification, and Utilization of Murine Monoclonal IgM Antibodies. Preparation and Use of Immunoaffinity Columns with Monoclonal Antibodies Without Purification from Ascites and Tissue-Culture Medium. Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography. Coupling of Monoclonal Antibodies with Fluorophores. Coupling of Monoclonal Antibodies with Biotin. Coupling of Monoclonal Antibodies with Enzymes. Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Negative Selection. Use of Monoclonal Antibodies with Magnetic Particlesto Separate Cell Subpopulations by Positive Selection. Index.

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