Morphology Methods: Cell and Molecular Biology Techniques

Overview

This text explores the most significant aspects of sophisticated and recent cell biological techniques that are needed to keep up with research in morphology. Researchers in morphology will find many step-by-step protocols to conduct their work including state-of {the {art laser capture microdissection methods, real time PCR and advanced FISH techniques. The chapters include detailed protocols for setting up and performing these techniques. This book covers all of the major areas relevant to molecular morphology ...

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Overview

This text explores the most significant aspects of sophisticated and recent cell biological techniques that are needed to keep up with research in morphology. Researchers in morphology will find many step-by-step protocols to conduct their work including state-of {the {art laser capture microdissection methods, real time PCR and advanced FISH techniques. The chapters include detailed protocols for setting up and performing these techniques. This book covers all of the major areas relevant to molecular morphology today. Potential pitfalls and anticipated problems are also discussed. Morphology Methods will prove essential to researchers interested in molecular morphology for the purpose of experiments, and for teaching graduate, or professional students wishing to keep up with the literature.

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Editorial Reviews

Booknews
This reference for pathologists, histologists, anatomists, and cell biologists presents molecular and cell biologic techniques used in the analysis of cells and tissues. Lloyd (Mayo Clinic) and other international researchers and clinicians describe techniques such as laser capture microdissection, Fluorescent Hybridization (FISH), confocal laser scanning microscopy, and tyramide amplification. Applications discussed include the diagnosis of endocrine and lymphoid lesions, the detection of infectious agents and DNA, and clonality analysis. The text is accompanied throughout by illustrations in black and white and color. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Doody's Review Service
Reviewer: Valerie L. Ng, PhD MD(Alameda County Medical Center/Highland Hospital)
Description: This a compendium of modern molecular techniques applicable to histology and pathology.
Purpose: The authors' goal is to provide an overview of the use of modern molecular technology for any application (clinical or research) in which tissue examination is performed. This objective was foreseen by the rapid explosion of molecular methodology now applicable to tissue examination.
Audience: The book is written for anyone who examines tissue and is interested in what is happening at the molecular level. It would thus be of interest to practicing pathologists, histotechnologists, researchers, graduate students, and technical research staff.
Features: The book nicely covers the hot topics in this area — laser capture microdissection, in situ hybridization, FISH, tyramide amplification methods, etc. An attractive feature of this book is that the last 10 chapters (of the 20) contain actual "cookbook" protocols (e.g., PCR, confocal laser scanning microscopy, immunohistochemistry, antigen retrieval, etc.) that can be adapted immediately to any laboratory. A glaring deficit in this book is the paucity of color figures to illustrate the power of the methodologies. The figures that are included are reproduced in black and white in the appropriate area in each chapter, yet the corresponding color figure is grouped into a single section containing all the color figures in the middle of the book. This creates some small confusion; for example, the figure legend of a black-and-white photo of a successful FISH experiment refers to the blending of colors in the abnormal tissue, yet the color photo is in another section and lacks the figure legend. This arrangement leads to lots of page flipping to get the most benefit from the figures.
Assessment: This book would be useful for those needing a concise reference for how to perform modern molecular tissue-based technology.

3 Stars from Doody
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Product Details

  • ISBN-13: 9781617372735
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/9/2010
  • Edition description: Softcover reprint of hardcover 1st ed. 2001
  • Edition number: 1
  • Pages: 422
  • Product dimensions: 0.91 (w) x 10.00 (h) x 7.00 (d)

Table of Contents

Introduction to Molecular Methods
Ricardo V. Lloyd

Laser Capture Microdissection: Principles and Applications
Ricardo V. Lloyd (Prools: H&E Staining, Operating the PIX Cell System; DNA Extraction from Collected Cells; RNA Extraction from Collected Cells)

In Situ Hybridization: Detection of DNA and RNA
Long Jin, Xiang Qian, and Ricardo V. Lloyd (Prool: In Situ Hybridization)

In Situ Detection of Infectious Agents
Gary W. Procop and Randall Hayden (Prools: Detecting Adenovirus, Epstein-Barr Virus)

Fluorescent In Situ Hybridization (FISH)
Susan Sheldon (Prool: FISH Materials)

Practical Applications of the FISH Technique
George Kontogeorgos, Nikiforos Kapranos, and Eleni Thodou (Prools: Metaphase Analysis from Whole Blood Culture; Metaphase Analysis from Solid Tumors; Interphase Analysis on Cell Imprints-Cytology Specimens; Interphase Analysis of Formalin-Fixed Paraffin-Embedded Tissues)

Tyramide Amplification Methods for In Situ Hybridization
John L. Frater and Raymond R. Tubbs (Prool: TSA, 1995; CARD/TSA with Nanogold; Tyramide-enhanced FISH with Peroxidase-labeled Oligonucleotides)

Prools: Ultrastructural In Situ Hybridization
Akira Matsuno, Tadashi Nagashima, R. Yoshiyuki Osamura, and Keiichi Watanabe

Quantitation of In Situ Hybridization Analysis
Lars-Inge Larsson

Prools: Confocal Laser Scanning Microscopy
Akira Matsuno, Johbu Itoh, Tadashi Nagashima, R. Yoshiyuki Osamura, and Keiichi Watanabe

Prools: Polymerase Chain Reaction
Yuri E. Nikiforov and Philip N. Howles

Prools: Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction
Omar Bagasra, Lisa E. Bobroski, and Muhammad Amjad

Immunohishemistry: Theory and Practice
Patrick C. Roche and Eric D. His

Antigen Retrieval Technique for Immunohishemistry: Principles, Prools, and Further Development
Clive R. Taylor and Shan-Rong Shi (Prool: Antigen Retrieval (AR) Technique)

Tyramide Amplification in Immunohishemistry
Naoko Sanno, Akira Teramoto, and R. Yoshiyuki Osamura (Prool: CARD Immunohishemistry)

Application of Immunohishemistry in the Diagnosis of Lymphoid Lesions
Paul J. Kurtin

Applications of Immunohishemistry in the Diagnosis of Undifferentiated Tumors
Mark R. Wick and Lisa A

Cerilli. Applications of Immunohishemistry in the Diagnosis of Endocrine Lesions
Ricardo V. Lloyd

Ultrastructural Immunohishemistry
Sergio Vidal, Eva Horvath, and Kalman Kovacs (Prool: Ultrastructural Immunohishemistry)

Prools: Clonality Analysis,
Aurel Perren and Paul Komminoth

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