Mouse Cell Culture: Methods and Protocols / Edition 1

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Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Prools, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including prools for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as prools for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Mouse Cell Culture: Methods and Prools serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.

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Editorial Reviews

From the Publisher
From the reviews:

“This book provides a diverse collection of prools for mouse cell culture in a text comprising 18 chapters. … This book is recommended for researchers and students who are interested in studying the culture of cells from animal models.” (Elvira M. Guerra Shinohara, Brazilian Journal of Pharmaceutical Sciences, October-December, 2010)

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Product Details

  • ISBN-13: 9781588297723
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 8/28/2010
  • Series: Methods in Molecular Biology Series, #633
  • Edition description: 2010
  • Edition number: 1
  • Pages: 250
  • Product dimensions: 7.20 (w) x 10.10 (h) x 0.80 (d)

Table of Contents

1. The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies
Melany Jackson, A. Helen Taylor, Elizabeth A. Jones, and Lesley M. Forrester

2. Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures
Alastair S. Garfield

3. Embryonic Skeletal Muscle Micro-Explant Culture and Isolation of Skeletal Muscle Stem Cells
Janet Smith and Deborah Merrick

4. The Embryonic Kidney: Isolation, Organ Culture, Immunostaining, and RNA Interference
Jamie A. Davies

5. Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation
Pierre-Marie del Moral and David Warburton

6. Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine
Jonathan M. Quinlan, Wei-Yuan Yu, and David Tosh

7. Isolation and Culture of Embryonic Pancreas and Liver
Zoë D. Burke, Wan-Chun Li, Jonathan M.W. Slack, and David Tosh

8. Isolation and Culture of Mouse Satellite Cells
Antonio Musarò and Laura Barberi

9. Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Prool for Preparation of Neonatal Mouse Cardiac Myocyte Cultures
Nigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, and Paul J.R. Barton

10. Short and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes
Reto Caldelari and Eliane J. Müller

11. Isolation, Culture, and Analysis of Mouse Mammary Epithelial Cells
Matthew J. Smalley

12. Isolation and Culture of Mouse Pancreatic Islets for ex vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes
Magalie A. Ravier and Guy A. Rutter

13. Isolation and Culture of Adult Mouse Hepaytes
Wan-ChunLi, Kate L. Ralphs, and David Tosh

14. Isolation and Culture of Mouse Intestinal Cells
F. Charles Campbell

15. Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse
Trevelyan R. Menheniott, Marika Charalambous, and Andrew Ward

16. The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cyhemistry
Carla Sciarretta and Liliana Minichiello

17. Cell Culture of Primary Cerebellar Granule Cells
Dana Krämer and Liliana Minichiello

18. Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Henrik Ahlenius and Zaal Kokaia

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