Mouse Hematology: A Laboratory Manual

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Overview


The mouse has become a standard laboratory model organism, particularly for the study of hematopoiesis, the immune system, and inflammation. Although laboratories studying stem cells, blood, and blood-forming tissues have assimilated many new molecular diagnostic methods, the identification of cell lineages through classical light microscopic techniques is often poorly understood and practiced. Mouse Hematology presents a concise review of conventional methods for the preparation, enumeration, and microscopic examination of blood and blood-forming tissues of the laboratory mouse. Along with a short laboratory manual featuring detailed protocols, Mouse Hematology includes a DVD of short video demonstrations of the techniques and a poster of blood cell types for easy identification at the microscope. These rapid, inexpensive assessments can save valuable time and resources essential to the design, development, and interpretation of experiments
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Product Details

  • ISBN-13: 9780879698867
  • Publisher: Cold Spring Harbor Laboratory Press
  • Publication date: 12/28/2009
  • Edition description: Lab Manual
  • Pages: 112
  • Product dimensions: 10.00 (w) x 12.50 (h) x 0.10 (d)

Table of Contents

Preface
1. Collection of Peripheral Blood
2. Counting Red Blood Cells, Platelets, and Viable Nucleated White Blood Cells
3. Peripheral Blood Films and Cytospin Preparations
4. Cell Differential Assessments of Peripheral Blood Films
5. Preparation of Bone Marrow for Microscopic Examination
6. Cell Differential Assessments of Bone Marrow
Conclusion
References
Cautions
Index

Videos on Accompanying DVD
Video 1 Venous Access for Blood Film Using Lateral Tail Vein
Video 2 Venous Access for Blood Film Using Tip of Tail
Video 3 Blood Film
Video 4 Staining with Coplin Jar or Carriages
Video 5 Coverslip
Video 6 Cytocentrifuge Procedures
Video 7 Surgical Exposure of Femur
Video 8 Brush Smear of Marrow

Poster
Mouse Peripheral Blood Cells
A quick reference guide to mouse peripheral blood cells, including brief descriptions of each lineage, total cellularities, and hematological/physiological parameters of interest.
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