Mouse Molecular Embryology: Methods and Protocols / Edition 1

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Overview

In Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study mouse embryology. These include protocols and techniques that are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA. With additional chapterson fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research.

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Product Details

  • ISBN-13: 9781603272902
  • Publisher: Springer US
  • Publication date: 11/30/2013
  • Series: Methods in Molecular Biology Series , #1092
  • Edition description: 2014
  • Edition number: 1
  • Pages: 435
  • Product dimensions: 7.10 (w) x 10.10 (h) x 1.20 (d)

Table of Contents

1. In situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional â-galactosidase Staining

Yoshihiro Komatsu, Satoshi Kishigami, and Yuji Mishina

2. Two Color In Situ Hybridization of Whole-Mount Mouse Embryos

Kristin K. Biris and Terry P. Yamaguchi

3. Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections

Davide De Pietri Tonelli, Yoanne M. Clovis and Wieland B. Huttner

4. Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays

Latasha C. Redmond, Christopher J. Pang, Catherine Dumur, Jack L. Haar and Joyce A. Lloyd

5. EMAGE: Electronic Mouse Atlas of Gene Expression

Lorna Richardson, Peter Stevenson, Shanmugasundaram Venkataraman. Yiya Yang, Nick Burton, Jianguo Rao, Jeffrey H. Christiansen, Richard A. Baldock and Duncan R. Davidson

6. Real-time PCR Quantification of gene Expression in Embryonic Mouse Tissue

Eric Villalon, David J. Schulz and Samuel T. Waters

7. Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic Approach

Aimin Liu and Jonathan Eggenschwiler

8. Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene Expression

David A. F. Loebel, Tania Radziewic, Melinda Power, Joshua B. Studdert and Patrick P. L. Tam

9. Generation of Tissue Organoids by Compaction Reaggregation

Julie M. Sheridan and C. Clare Blackburn

10. Ultra-Rapid Vitrification of Mouse Oocytes and Embryos

Mark G. Larman PhD and David K. Gardner

11. Mammalian Preimplantation Embryo Culture

David K. Gardner and Michelle Lane

12. Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs

Julie Gordon, Billie A. Moore, C. Clare Blackburn and

Nancy R. Manley

13. Genetically Encoded Probes Provide a Window On Embryonic Arrhythmia

Yvonne Norine Tallini, Kai Su Greene, Bo Shui, Calum William Russell, Jane Constance Lee, Robert Michael Doran, Junichi Nakai and Michael I. Kotlikoff

14. Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model Organisms

Suresh I. Prajapati, Lisa Nevell and Charles Keller

15. Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse Embryos

Daisuke Sakai and Paul A Trainor

16. Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues

Kaustav Nandy, Jusub Kim, Dean P. McCullough, Matthew McAuliffe, Karen J. Meaburn, Terry P. Yamaguchi, Prabhakar R. Gudla and Stephen J. Lockett

17. Protein/Peptide Transduction in Metanephric Explant Culture

Sergey Plisov, Honghe Wang, Nadya Tarasova, Nirmala Sharma, and Alan O. Perantoni

18. Direction of Cells Programmed to Die in Mouse Embroys

Rocío Hernández-Martínez and Luis Covarrubias

19. Microscopic Computed Tomography-based Virtual Histology of Embryos

Suresh I. Prajapati, David R. Rodriguez, Charles Keller

20. Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study

Yelena Golubeva and David Symer

21. The Latest Improvements in the Mouse Sperm Preservation

Takehito Kaneko

22. Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitro

Satomi S. Tanaka, Yasuka L. Yamaguchi, Vanessa J. Jones and Patrick P. L. Tam

23. Using the Textpresso Site Specific Recombinases Web Server to Identify Cre Expressing Mouse Strains and Floxed Alleles

Brian G. Condie and William M. Urbanski

24. Live Imaging Mouse Embryonic Development: Seeing is Believing and Revealing

Sonja Nowotschin and Anna-Katerina Hadjantonakis

25. Genetic Cell Ablation

Damien Grégoire and Marie Kmita

26. Essentials of Recombinase-based Genetic Fate Mapping in Mice

Patricia Jensen and Susan M. Dymecki

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