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Doody's Review ServiceReviewer: Rebecca T. Horvat, PhD, D (ABMM)(University of Kansas Medical Center)
Description: Noting that the mycobacteria are "difficult organisms to work with," the editors provide this book with details on laboratory protocols that will be valuable to scientists working in this area. This first edition was published in 1998, and much has changed in the tools available to researchers. Thus, this book is needed to promote and enhance research in this area.
Purpose: This book will be a useful reference for scientists working with mycobacteria. These new techniques and ideas are valuable to laboratory researchers since many of these methods may take weeks or months to complete and involve expensive technologies. The book will be invaluable to these scientists.
Audience: Scientists with expertise in the study of mycobacteria are the intended audience, but the book also would be helpful for graduate students or postdoctoral fellows working at the bench. The authors include both senior scientists and bench scientists.
Features: The techniques include advice on the isolation of DNA and RNA from the thick-walled mycobacteria. The book includes methods for the isolation and study of the cell wall lipids and well as the investigation of global gene expression using microarrays and the introduction of plasmids using electroporation. Very detailed descriptions include a diagram of a continuous cell culture system for mycobacteria. This system allows for the investigation of how this bacterium responds to changes in environment. An additional system shows how to study mycobacteria inside the amoeba.
Assessment: This will be a very valuable resource for scientists working in the field of mycobacteria. It updates the previous edition with the techniques that have been developed in the last 10 years.