Myeloid Leukemia: Methods and Protocols / Edition 1

Hardcover (Print)
Buy New
Buy New from
Used and New from Other Sellers
Used and New from Other Sellers
from $1.99
Usually ships in 1-2 business days
(Save 99%)
Other sellers (Hardcover)
  • All (17) from $1.99   
  • New (13) from $1.99   
  • Used (4) from $11.16   


A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.

Read More Show Less

Product Details

  • ISBN-13: 9781588294852
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/28/2005
  • Series: Methods in Molecular Medicine Series, #125
  • Edition description: 2006
  • Edition number: 1
  • Pages: 320
  • Product dimensions: 6.20 (w) x 9.00 (h) x 1.10 (d)

Table of Contents

Isolation of RNA and DNA From Leukocytes and cDNA Synthesis

Joop H. Jansen and Bert A. van der Reijden

Cytogenetic and FISH Techniques in Myeloid Malignancies

Lynda J. Campbell

Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Malignancies

Christophe Picard, Monique Silvy, and Jean Gabert

Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR

Susan Branford and Timothy Hughes

Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate

Susan Branford and Timothy Hughes

Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemia

Lynda J. Campbell

Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCR

Vincenzo Rossi, Laura Levati, and Andrea Biondi

Diagnosis and Monitoring of PML-RARa-Positive Acute Promyelocytic Leukemia by Quantitative RT-PCR

Elisa Mokany, Alison V. Todd, Caroline J. Fuery, and Tanya L. Applegate

Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-Time Quantitative RT-PCR

Khalid Tobal and John A. Liu Yin

Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitative RT-PCR

Bert A. van der Reijden and Joop H. Jansen

Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophilic Leukemia

Jan Cools, Elizabeth H. Stover, and D. Gary Gilliland

FLT3 Mutations in Acute Myeloid Leukemia

Hitoshi Kiyoi and Tomoki Naoe

WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes

Daniela Cilloni, Enrico Gottardi, and Giuseppe Saglio

Classification of AML by DNA-Oligonucleotide Microarrays

Alexander Kohlmann, Wolfgang Kern, Wolfgang Hiddemann, and Torsten Haferlach

Classification of AML Using a Monoclonal Antibody Microarray

Richard I. Christopherson, Kerryn Stoner, Nicole Barber, Larissa Belov, Adrian Woolfson, Mike Scott, Linda Bendall, and Stephen P. Mulligan

Methods for the Detection of the JAK2 V617F Mutation in Human Myeloproliferative Disorders

Peter J. Campbell, Linda M. Scott, E. Joanna Baxter, Anthony J. Bench, Anthony R. Green, and Wendy N. Erber

Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemia

Maurizio Martini, Luciana Teofili, and Luigi M. Larocca

Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantation

Thomas Lion and Franz Watzinger


Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)