Myeloid Leukemia: Methods and Protocols / Edition 1

Myeloid Leukemia: Methods and Protocols / Edition 1

by Harry Iland
     
 

ISBN-10: 1588294854

ISBN-13: 9781588294852

Pub. Date: 11/28/2005

Publisher: Springer-Verlag New York, LLC

A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and

…  See more details below

Overview

A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.

Product Details

ISBN-13:
9781588294852
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/28/2005
Series:
Methods in Molecular Medicine Series, #125
Edition description:
2006
Pages:
320
Product dimensions:
6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Isolation of RNA and DNA From Leukocytes and cDNA Synthesis

Joop H. Jansen and Bert A. van der Reijden

Cytogenetic and FISH Techniques in Myeloid Malignancies

Lynda J. Campbell

Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Malignancies

Christophe Picard, Monique Silvy, and Jean Gabert

Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR

Susan Branford and Timothy Hughes

Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate

Susan Branford and Timothy Hughes

Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemia

Lynda J. Campbell

Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCR

Vincenzo Rossi, Laura Levati, and Andrea Biondi

Diagnosis and Monitoring of PML-RARa-Positive Acute Promyelocytic Leukemia by Quantitative RT-PCR

Elisa Mokany, Alison V. Todd, Caroline J. Fuery, and Tanya L. Applegate

Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-Time Quantitative RT-PCR

Khalid Tobal and John A. Liu Yin

Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitative RT-PCR

Bert A. van der Reijden and Joop H. Jansen

Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophilic Leukemia

Jan Cools, Elizabeth H. Stover, and D. Gary Gilliland

FLT3 Mutations in Acute Myeloid Leukemia

Hitoshi Kiyoi and Tomoki Naoe

WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes

Daniela Cilloni, Enrico Gottardi, and Giuseppe Saglio

Classification of AML by DNA-Oligonucleotide Microarrays

Alexander Kohlmann, Wolfgang Kern, Wolfgang Hiddemann, and Torsten Haferlach

Classification of AML Using a Monoclonal Antibody Microarray

Richard I. Christopherson, Kerryn Stoner, Nicole Barber, Larissa Belov, Adrian Woolfson, Mike Scott, Linda Bendall, and Stephen P. Mulligan

Methods for the Detection of the JAK2 V617F Mutation in Human Myeloproliferative Disorders

Peter J. Campbell, Linda M. Scott, E. Joanna Baxter, Anthony J. Bench, Anthony R. Green, and Wendy N. Erber

Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemia

Maurizio Martini, Luciana Teofili, and Luigi M. Larocca

Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantation

Thomas Lion and Franz Watzinger

Index

Read More

Customer Reviews

Average Review:

Write a Review

and post it to your social network

     

Most Helpful Customer Reviews

See all customer reviews >