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Necrosis: Methods and Protocols

Overview

Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Prools experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in ...

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Overview

Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Prools experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Necrosis: Methods and Prools describes techniques in an easy to follow manner, with details so that beginners can succeed with challenging techniques.

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Product Details

  • ISBN-13: 9781627033824
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 6/30/2013
  • Series: Methods in Molecular Biology Series , #1004
  • Edition description: 2013
  • Edition number: 1
  • Pages: 252
  • Product dimensions: 7.00 (w) x 10.00 (h) x 0.80 (d)

Table of Contents

Part I: Analysis of Necrosis In Vitro
1. Navigation to the Graveyard- Induction of Various Pathways of Necrosis and their Classification by Flow Cytometry
Christina Janko, Luis Munoz, Ricardo Chaurio, Christian Maueröder, Christian Berens, Kirsten Lauber, Martin Herrmann
2. Time-lapse Imaging of Necrosis
Fredrik Wållberg, Tencho Tenev, and Pascal Meier
3. Activity Assays for Receptor Interacting Protein Kinase 1: A Key Regulator of Necroptosis
Jenny L. Maki and Alexei Degterev
4. Fluorescent Biosensors for the Detection of HMGB1 Release
Isabelle Martins, Oliver Kepp, Laurie Menger, Mickael Michaud, Sandy Adjemian, Abdul Qader Sukkurwala, Erika Vacchelli, Lorenzo Galluzzi , and Guido Kroemer
Part II: Alternative Cell Death Modalities
5. Methods for the Study of Entosis
Qiang Sun and Michael Overholtzer
6. Analysis of Pyroptosis in Bacterial Infection
Lia Danelishvili and Luiz E Bermudez
Part III: Analysis of Cell Death in Mammalian Tissues
7. Analysis of Cell Death by Electron Microscopy
S. Burattini and E. Falcieri
8. Histological Analysis of Neurodegeneration in the Mouse Brain
Hiroo Yamaguchi and Jie Shen
9. Quantitation of Acute Necrosis after Experimental Myocardial Infarction
Xin-Yi Yeap, Shirley Dehn, Jeremy Adelman, Jeremy Lipsitz, & Edward B. Thorp
10. Immunohistological Tools to Discriminate Apoptotic and Necrotic Cell Death in the Skin
Marion C. Bonnet
Part IV: Cell Death in Non-Mammalian Model Systems
11. Identification and Characterization of Programmed Cell Death Markers in Bacterial Models
Daniel J. Dwyer and Jonathan A. Winkler
12. Quantification of Genetically Controlled Cell Death in Budding Yeast
Xinchen Teng and J. Marie Hardwick
13. Necrosis in C. elegans
Matt Crook, Avni Upadhyay and Wendy Hanna-Rose
14. Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans
Zao Li, Nan Lu, Xiangwei He, and Zheng Zhou
15. Modeling Calcium-overload Mediated Necrosis in Drosophila
Kai Liu, Yuhong Li and Lei Liu
16. Use of Necrotic Markers in the Drosophila Ovary
Allison K. Timmons, Tracy L. Meehan, Tori D. Gartmond, and Kimberly McCall
17. Detection and Measurement of Necrosis in Plants
Elena A. Minina, Lada H. Filonova, Geoffrey Daniel, and Peter V. Bozhkov

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