Neoglycoconjugates, Part B: Biomedical Applications / Edition 1

by John N. Abelson, Melvin I. Simon, Y. C. Lee, Reiko T. Lee

Overview

Neoglycoconjugates are not only useful for the basic understanding of protein-carbohydrate interactions, but they have many practical applications as well. They are powerful reagents in many cell biology studies and excellent tools for the isolation and characterization of animal and plant lectins, separation of cells, as well as for the targeting of drugs, artificial vaccines, and diagnostic reagents. Volume 247 and its companion Volume 242 contain many practical methods on how to prepare and use neoglycoconjugates. Volume 242 deals with synthesis and 247 with biomedical applications.

Product Details

ISBN-13:	9780121821487
Publisher:	Elsevier Science
Publication date:	12/05/1994
Series:	Methods in Enzymology , #247
Pages:	450
Product dimensions:	5.94(w) x 9.00(h) x (d)

About the Author

Lee, Y. C. (Johns Hopkins Univ); Lee, Reiko T. (Johns Hopkins Univ)

The contributors represent the specialties of chemisty, biochemistry, molecular biology, molecular genetics, nuclear medicine, immunology and pharmaceutics. They are an international group from the U.S., China, Germany, Japan, Canada, Poland, and Sweden. Institutions prominently represented include UC Davis, Ohio State Univ, Nagoya Univ School of Medicine, National Institutes of Health, and the Univ of Ottowa.

Table of Contents

Oligosaccharide Derivatives and Glycopeptides:
H. Kunz and K. von dem Bruch, Neoglycoproteins from Synthetic Glycopeptides.
K.G. Rice, Preparation of Fluorescent Glycoconjugates for Energy Transfer Studies.
T. Tamura, M.S. Wadhwa, M.H. Chiu, M.L.C. Da Silva, T. McBoom, and K.G. Rice, Preparation of Tyrosinamide-Oligosaccharides as Iodinatable Glycoconjugates.
T. Yoshida, Glycamine Formation via Reductive Amination of Oligosaccharides with Benzylamine.
K. Koizumi, S. Kitahata, and H. Hashimoto, Preparation, Isolation, and Analysis of Heterogeneous Branched Cyclodextrins.
T. Norborg, B. Laning, and J. Tejbrant, Solid-Phase Synthesis of O-Glycopeptides.
Y. Ichikawa, R. Wang, and C.-H. Wong, Regeneration of Sugar Nucleotide for Enzymatic Oligosaccharide Synthesis.
S. Ogawa, Chemical Synthesis of Core Structures of Oligosaccharide Chains of Cell Surface Glycans Containing Carba Sugars.
S. Ogawa and H. Tsunoda, Chemical Synthesis of Glycosylamide and Cerebroside Analogs Composed of Carba Sugars.
J.J. Krepinsky, D.M. Whitfield, S.P. Douglas, N. Lupescu, D. Pulleyblank, and F.L. Moolten, Galactosylation of Nucleosides at 5(- Position of Pentofuranoses.
R. Brossmer and H.J. Gross, Sialic Acid Analogs and Application for Preparation of Neoglycoconjugates.
R. Brossmer and H.J. Gross, Fluorescent and Photoactivatable Sialic Acids.
S. Aoki, H. Kondo, and C.-H. Wong, Glycosyl Phosphites as Glycosylation Reagents.
Enzymatic and Affinity Methods:
M.M. Palcic, M. Pierce, and O. Hindsgaul, Synthetic Neoglycoconjugates in Glycosyltransferase Assay and Purification.
A. Kobata, N. Kochibe, and T. Endo, Affinity Chromatography of Oligosaccharides on Psathyrella velutina Lectin Column.
S. Sabesan, J.C. Paulson, and J. Weinstein, Separation of Galb1, 4G1cNAc a-2,6- and Galb1,3(4)GlcNAc a-2,3-Sialyltransferases by Affinity Chromatography.
E. Altman and D.R. Bundle, Polysaccharide Affinity Columns for Purification of Lipopolysaccharide-Specific Murine Monoclonal Antibodies.
M.-C. Shao and C.Q.C. Chin, Streptavidin-Biotinylglycopeptide-Lectin Complex in Detection of Glycopeptides and Determination of Lectin Specificity.
Binding Site Characterization:
J. Lehmann and M. Schmidt-Schuchardt, Spacer-Modified Oligosaccharides as Photoaffinity Probes for Porcine Pancreatic (-Amylase.
D.R. Bundle and B.W. Sigurskjold, Determination of Accurate Thermodynamics of Binding by Titration Microcalorimetry.
C.P.J. Glaudemans, P. Kovac, and E.M. Nashed, Mapping of Hydrogen Bonding between Saccharides and Proteins in Solution.
Biomedical Applications:
T. Toyokuni and S.-I. Hakomori, Carbohydrate(Lysyllysine Conjugates as Cell Antiadhesion Agents.
M.A. Findeis, C.H. Wu, and G.Y. Wu, Ligand-Based Carrier Systems for Delivery of DNA to Hepatocytes.
R. Roy, C.A. Laferriore, R.A. Pon, and A. Gamian, Introduction of Rabbit Immunoglobulin G Antibodies against Synthetic Sialylated Neoglycoproteins.
N. Sugiura and K. Kimata, Syntheses and Functions of Neoproteoglycans: Lipid-Derivatized Chondroitin Sulfate with Antiadhesion Activity.
M. Kudo, D.R. Vera, and R.C. Stadalnik, In Vivo Quantification of Asialoglycoprotein Receptor.
M. Kudo, K. Washino, Y. Yamamichi, and K. Ikekubo, Synthesis and Radiolabeling of Galactosyl Human Serum Albumin.
D.R. Vera, S.J. Topcu, and R.C. Stadalnik, In Vitro Quantification of Asialoglycoprotein Receptor Density from Human Hepatic Microsamples.
D.R. Vera, R.C. Stadalnik, M. Kudo, and K.A. Krohn, Radiopharmaceutical Preparation of Technetium-99m-Labeled Galactosyl-Neoglycoalbumin.
K. Kobayashi, A. Kobayashi, and T. Akaike, Culturing Hepatocytes on Lactose-Carrying Polystyrene Layer via Asialoglycoprotein Receptor-Mediated Interactions.
Author Index.
Subject Index.

Editorial Reviews

Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
—NEUROSCIENCE
"Incomparably useful."
—ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
—BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
—CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
—AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
—ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
—JOURNAL OF CHROMATOGRAPHY

From the Publisher

Reviewer: C. Harry Arsenis, PhD (Scholl Chicago Foot Health Centers)
Description: This volume in the Methods in Enzymology series deals with the biomedical applications of this group of neoglycoconjugates. There are several chapters dealing with the synthesis of conjugates attached to lipids, proteins and insoluble carbohydrates.
Purpose: The purpose is to describe the applications of different neoglycoconjugates bound to different materials of lipid, protein, or carbohydrate origin. The conjugated moieties have a natural origin or have been synthetically made. The objectives of the book are particularly worthy in view of the recent interest in the use of natural products in medicine and industry. The specific objective of the book is to describe the applications of the chemically synthesized or natural glycoconjugates. The book meets the authors' objectives.
Audience: The book is written for chemists and other research scientists who are in search of synthetic or natural matrices for the testing of different ligands in medicine or industry. The book will additionally be interesting to diversified scientists in various industries who are looking for an effective "Bed." The methodologies described provide accurate procedures for the application of chemomimetics for laboratory or industrial use. The book is written for the advanced student or researcher specialist in search of a matrix-ligand combination suitable for his or her system of study. The neoglycoconjugates may find applications in a variety of industries not related to medicine or drug development.
Features: The book is adequately illustrated. The references are quite current (latest 1993) and pertinent for the subject.
Assessment: Cells communicate with their environment by synthesizing and using as sensing devices a variety of carbohydrate-based molecules. These moieties are attached to various fixed or insoluble structures, such as for example, membranes. Consequently, the chemical synthesis or isolation in pure form of these conjugates is a valuable system for research and development in both medicine and industry. The research and exploration of these carbohydrate conjugates necessitates the attachment of these ligands onto some structure resembling their natural carrier, a protein, a lipid, or another insoluble carbohydrate. Consequently, the methodology on the applications of these conjugates is extremely essential. The research and application of the findings demand that these products are immobilized or attached to various matrices. The book provides a useful compilation of the methodologies for testing and using these materials (the conjugates). The dedication of a separate volume on the application of the neoglycoconjugates seems to be a good idea.

Doody's Review Service

Reviewer: C. Harry Arsenis, PhD(Scholl Chicago Foot Health Centers)
Description: This volume in the Methods in Enzymology series deals with the biomedical applications of this group of neoglycoconjugates. There are several chapters dealing with the synthesis of conjugates attached to lipids, proteins and insoluble carbohydrates.
Purpose: The purpose is to describe the applications of different neoglycoconjugates bound to different materials of lipid, protein, or carbohydrate origin. The conjugated moieties have a natural origin or have been synthetically made. The objectives of the book are particularly worthy in view of the recent interest in the use of natural products in medicine and industry. The specific objective of the book is to describe the applications of the chemically synthesized or natural glycoconjugates. The book meets the authors' objectives.
Audience: The book is written for chemists and other research scientists who are in search of synthetic or natural matrices for the testing of different ligands in medicine or industry. The book will additionally be interesting to diversified scientists in various industries who are looking for an effective "Bed." The methodologies described provide accurate procedures for the application of chemomimetics for laboratory or industrial use. The book is written for the advanced student or researcher specialist in search of a matrix-ligand combination suitable for his or her system of study. The neoglycoconjugates may find applications in a variety of industries not related to medicine or drug development.
Features: The book is adequately illustrated. The references are quite current (latest 1993) and pertinent for the subject.
Assessment: Cells communicate with their environment by synthesizing and using as sensing devices a variety of carbohydrate-based molecules. These moieties are attached to various fixed or insoluble structures, such as for example, membranes. Consequently, the chemical synthesis or isolation in pure form of these conjugates is a valuable system for research and development in both medicine and industry. The research and exploration of these carbohydrate conjugates necessitates the attachment of these ligands onto some structure resembling their natural carrier, a protein, a lipid, or another insoluble carbohydrate. Consequently, the methodology on the applications of these conjugates is extremely essential. The research and application of the findings demand that these products are immobilized or attached to various matrices. The book provides a useful compilation of the methodologies for testing and using these materials (the conjugates). The dedication of a separate volume on the application of the neoglycoconjugates seems to be a good idea.

This volume in the Methods in Enzymology series deals with the biomedical applications of this group of neoglycoconjugates. There are several chapters dealing with the synthesis of conjugates attached to lipids, proteins and insoluble carbohydrates. The purpose is to describe the applications of different neoglycoconjugates bound to different materials of lipid, protein, or carbohydrate origin. The conjugated moieties have a natural origin or have been synthetically made. The objectives of the book are particularly worthy in view of the recent interest in the use of natural products in medicine and industry. The specific objective of the book is to describe the applications of the chemically synthesized or natural glycoconjugates. The book meets the authors' objectives. The book is written for chemists and other research scientists who are in search of synthetic or natural matrices for the testing of different ligands in medicine or industry. The book will additionally be interesting to diversified scientists in various industries who are looking for an effective "Bed. The methodologies described provide accurate procedures for the application of chemomimetics for laboratory or industrial use. The book is written for the advanced student or researcher specialist in search of a matrix-ligand combination suitable for his or her system of study. The neoglycoconjugates may find applications in a variety of industries not related to medicine or drug development. The book is adequately illustrated. The references are quite current (latest 1993) and pertinent for the subject. Cells communicate with their environment by synthesizing and using as sensing devices a variety of carbohydrate-basedmolecules. These moieties are attached to various fixed or insoluble structures, such as for example, membranes. Consequently, the chemical synthesis or isolation in pure form of these conjugates is a valuable system for research and development in both medicine and industry. The research and exploration of these carbohydrate conjugates necessitates the attachment of these ligands onto some structure resembling their natural carrier, a protein, a lipid, or another insoluble carbohydrate. Consequently, the methodology on the applications of these conjugates is extremely essential. The research and application of the findings demand that these products are immobilized or attached to various matrices. The book provides a useful compilation of the methodologies for testing and using these materials (the conjugates). The dedication of a separate volume on the application of the neoglycoconjugates seems to be a good idea.

C. Harry Arsenis