Neural Stem Cells: Methods and Protocols / Edition 2

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*Focus on clinical and translational relevance *New and revised chapters that include neural stem cell markers, cytogenetics, and polymerase chain reactions, among others Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular BiologyT series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases. Written for: Stem cell scientists, neuroscientists, investigators, fellows, and students

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Editorial Reviews

Doody's Review Service
Reviewer: Celso Agner, MD, MS, MSc (Michigan Neurology Partners)
Description: Neural stem cells have been the focus of much research in an attempt to better understand the nature and behavior of these cells in vivo. In addition, potential cure of degenerative neurological disorders has challenged both scientists and the lay community. Since the first edition of this book, much has changed in the laboratory techniques and understanding of neural stem cells. A second edition was, therefore, much needed.
Purpose: The purpose is to clarify research protocols in the investigation of neural stem cells. These worthy objectives are met.
Audience: Neurologists, neurosurgeons, and neuroscientists are the main audience.
Features: Divided into four sections, the book addresses cell culture, cellular, molecular, and transplantation techniques. Despite being primarily a technical book outlining many bench laboratory techniques, the last section describes a possible use for stem cells in the management of neurological conditions in the future. Neural stem cell transplantation may find its way to control neoplasms, aggressive multiple sclerosis, Parkinson's disease, and other conditions in the decades to come.
Assessment: This is a valuable acquisition for academic neuroscience, neurology, and neurosurgery libraries.
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Product Details

  • ISBN-13: 9781588298461
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 2/28/2008
  • Series: Methods in Molecular Biology Series, #438
  • Edition description: 2nd ed. 2008
  • Edition number: 2
  • Pages: 406
  • Product dimensions: 6.11 (w) x 9.54 (h) x 1.13 (d)

Table of Contents

Preface     v
Contributors     xi
Definitions and Criteria for Stem Cells   Leslie P. Weiner     3
Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard   Jaime Imitola   Samia J. Khoury     9
Isolation and Culture of NSCs
Neural Differentiation of Human Embryonic Stem Cells   Mirella Dottori   Martin F. Pera     19
Generating Neurons from Stem Cells   Andreas Androutsellis-Theotokis   Sachiko Murase   Justin D. Boyd   Deric M. Park   Daniel J. Hoeppner   Rea Ravin   Ronald D. G. McKay     31
Isolation of Neural Stem and Precursor Cells from Rodent Tissue   Yuanyuan Wu   Ying Liu   Jonathan D. Chesnut   Mahendra S. Rao     39
Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization   Lamya S. Shihabuddin     55
Adult Neural Stem Cells   Rossella Galli   Angela Gritti   Angelo L. Vescovi     67
Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb   Susan C. Barnett   A. Jane Roskams     85
Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium   Miranda Richter   Kathryn Westendorf   A. Jane Roskams     95
Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property   Seung U. Kim   Atsushi Nagai   Eiji Nakagawa   Hyun B. Choi   Jung H. Bang   Hong J. Lee   Myung A. Lee   Yong B. Lee   In H. Park     103
Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood   Shijie Song   Juan Sanchez-Ramos     123
Production of Neurospheres from CNS Tissue   Gregory P. Marshall II   Heather H. Ross   Oleg Suslov   Tong Zheng   Dennis A. Steindler   Eric D. Laywell     135
Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells   Shigeo Saito   Ken Sawai   Yoshinobu Murayama   Keiichi Fukuda   Kazunari Yokoyama     151
In Vitro Studies: A. Cellular Techniques
Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures   Angela Gritti   Rossella Galli   Angelo L. Vescovi     173
Telomere Neurobiology   Mark P. Mattson   Peisu Zhang   Aiwu Cheng     185
Chromosomal Mosaicism in Neural Stem Cells   Suzanne E. Peterson   Jurjen W. Westra   Christine M. Paczkowski   Jerold Chun     197
In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype   Marcel M. Daadi     205
In Vitro Migration Assays of Neural Stem Cells   Pascale Durbec   Isabelle Franceschini   Francoise Lazarini   Monique Dubois-Dalcq     213
Measuring Apoptosis in Neural Stem Cells   Brett T. Lund   Eve E. Kelland     227
Markers of Adult Neural Stem Cells   Eric Wexler     243
In Vitro Studies: B. Molecular Techniques
Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR   Nicole Slawny   Crystal Pacut   Theresa E. Gratsch     271
Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells   Yan Chen   Jiang F. Zhong     293
Electroporation Strategies for Genetic Manipulation and Cell Labeling   Terence J. Van Raay   Rhonda T. Lassiter   Michael R. Stark     305
Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches   Luciano Conti   Elena Cattaneo     319
Studies for Transplantation
Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo   Sanjay S. Magavi   Jeffrey D. Macklis     335
Immunocytochemical Analysis of Neuronal Differentiation   Sanjay S. Magavi   Jeffrey D. Macklis     345
Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold   Lisa A. Catapano   Sanjay S. Magavi   Jeffrey D. Macklis     353
Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS   Qi-lin Cao   Stephen M. Onifer   Scott R. Whittemore     361
Optimizing Stem Cell Grafting into the CNS   Scott R. Whittemore   Yi Ping Zhang   Christopher B. Shields   David S. K. Magnuson     375
Routes of Stem Cell Administration in the Adult Rodent   Alison E. Willing   Svitlana Garbuzova-Davis   Paul R. Sanberg   Samuel Saporta     383
Index     403
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