Neural Stem Cells: Methods and Protocols / Edition 2

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Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells: Methods and Protocols, Second Edition, revises and expands on the successful first edition in order to provide the most current, cutting-edge methods for scientists working to answer these questions. Using these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means for understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new edition provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and current, Neural Stem Cells: Methods and Protocols, Second Edition, is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.

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Editorial Reviews

Doody's Review Service
Reviewer: Celso Agner, MD, MS, MSc (Michigan Neurology Partners)
Description: Neural stem cells have been the focus of much research in an attempt to better understand the nature and behavior of these cells in vivo. In addition, potential cure of degenerative neurological disorders has challenged both scientists and the lay community. Since the first edition of this book, much has changed in the laboratory techniques and understanding of neural stem cells. A second edition was, therefore, much needed.
Purpose: The purpose is to clarify research protocols in the investigation of neural stem cells. These worthy objectives are met.
Audience: Neurologists, neurosurgeons, and neuroscientists are the main audience.
Features: Divided into four sections, the book addresses cell culture, cellular, molecular, and transplantation techniques. Despite being primarily a technical book outlining many bench laboratory techniques, the last section describes a possible use for stem cells in the management of neurological conditions in the future. Neural stem cell transplantation may find its way to control neoplasms, aggressive multiple sclerosis, Parkinson's disease, and other conditions in the decades to come.
Assessment: This is a valuable acquisition for academic neuroscience, neurology, and neurosurgery libraries.
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Product Details

  • ISBN-13: 9781617378041
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/10/2010
  • Series: Methods in Molecular Biology Series, #438
  • Edition description: Softcover reprint of hardcover 2nd ed. 2008
  • Edition number: 2
  • Pages: 406
  • Product dimensions: 0.86 (w) x 9.21 (h) x 6.14 (d)

Table of Contents





1. Definitions and Criteria for Stem Cells

Leslie P. Weiner

2. Neural Stem Cells and the Future Treatment of Neurologic Diseases: Raising the Standard

Jaime Imitola and Samia J. Khoury


3. Neural Differentiation of Human Embryonic Stem Cells

Mirella Dottori and Martin F. Pera

4. Generating Neurons from Stem Cells

Andreas Androutsellis-Theotokis, Sachiko Murase, Justin D. Boyd, Deric M. Park, Daniel J. Hoeppner, Rea Ravin, and Ron McKay

5. Isolation of Neural Stem and Precursor Cells from Rodent Tissue

Yuanyuan Wu, Ying Liu, Jonathan D. Chesnut, and Mahendra S. Rao

6. Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization

Lamya S. Shihabuddin

7. Adult Neural Stem Cells

Rossella Galli, Angela Gritti, and Angelo L. Vescovi

8. Olfactory Ensheathing Cells: Isolation and Culture from the Olfactory Bulb and Olfactory Epithelium

Susan C. Barnett and Jane Roskams

9. Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property

Seung U. Kim, Atsushi Nagai, Eiji Nakagawa, Hyun B. Choi, Jung H. Bang, Hong J. Lee, Myung A. Lee, Yong B. Lee, and In H. Park

10. Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood

Shijie Song and J. Sanchez-Ramos

11. Production of Neurospheres from CNS Tissue

Gregory P. Marshall II, Oleg Suslov, Tong Zheng, Dennis A. Steindler, and Eric D. Laywell

12. Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells

Shigeo Saito, Ken Sawai, Yoshinobu Murayama, Keiichi Fukuda, and Kazunari Yokoyama



13. Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures

Angela Gritti, Rosella Galli and Angelo L. Vescovi

14. Telomere Neurobiology

Mark P. Mattson, Peisu Zhang and Aiwu Cheng

15. Chromosomal Mosaicism in Neural Stem Cells

Suzanne E. Peterson, Jurjen W. Westra, Christine M. Paczkowski, and Jerold Chun

16. In vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype

Marcel M. Daadi

17. In vitro Migration Assays of Neural Stem Cells

Pascale Durbec, Isabelle Franceschini, Francoise Lazarini, and Monique Dubois-Dalcq

18. Measuring Apoptosis in Neural Stem Cells

Brett T. Lund and Eve E. Kelland

19. Markers of Adult Neural Stem Cells

Eric Wexler


20. Differential Gene Expression in ES Derived Neural Stem Cells Using RT PCR

Nicole Slawny, Crystal Pacut, and Theresa E. Gratsch

21. Microfluidic Devices for High Throughput Gene Expression Profiling Single hESC-derived Neural Stem Cell

Yan Chen and Jiang F. Zhong

22. Electroporation Strategies for Genetic Manipulation and Cell Labeling

Terence J. Van Raay, Rhonda T. Lassiter, and Michael R. Stark

23. Novel and Immortalization-Based Prools for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches

Luciano Conti and Elena Cattaneo


24. Identification of Newborn Cells by BrdU Labeling and Immunocyhemistry In Vivo

Sanjay S. Magavi and Jeffrey D. Macklis

25. Immunocyhemical Analysis of Neuronal Differentiation

Sanjay S. Magavi and Jeffrey D. Macklis

26. Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold

Lisa A. Catapano, Sanjay S. Magavi, and Jeffrey D. Macklis

27. Labeling Stem Cells in Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS

Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore

28. Optimizing Stem Cell Grafting into the CNS

Scott R. Whittemore, Yi Ping Zhang, Christopher B. Shields and

David S. K. Magnuson

29. Routes of Stem Cell Administration in the Adult Rodent

Alison E. Willing, Svitlana Garbuzova-Davis, Paul R. Sanberg, and

Samuel Saporta

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