Neuronal Cell Culture: Methods and Protocols

Overview

In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced ...

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Overview

In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.

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Product Details

  • ISBN-13: 9781627036399
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 9/2/2013
  • Series: Methods in Molecular Biology Series , #1078
  • Edition description: 2013
  • Edition number: 1
  • Pages: 165
  • Product dimensions: 7.10 (w) x 10.10 (h) x 0.60 (d)

Table of Contents

1. General Overview of Neuronal Cell Culture

Jennifer Gordon, Shohreh Amini, and Martyn K. White

2. Considerations for the Use of SH-SY5Y Neuroblastoma Cells in Neurobiology

Jane Kovalevich and Dianne Langford

3. Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12

Nune Darbinian

4. Murine Teratocarcinoma-Derived Neuronal Cultures

Prasun K. Datta

5. Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons

Armine Darbinyan, Rafal Kaminski, Martyn K. White, Nune Darbinian, and Kamel Khalili

6. Mouse Enteric Neuronal Cell Culture

Yonggang Zhang and Wenhui Hu

7. Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications

Joseph F. Bonner, Christopher J. Haas, and Itzhak Fischer

8. Derivation of Neuronal Cells from Fetal Normal Human Astrocyte (NHA)

S. Ausim Azizi and Barbara Krynska

9. Slice Culture Modeling of Central Nervous System (CNS) Viral Infection

Kalen R. Dionne and Kenneth L. Tyler

10. Neurospheres and Glial Cell Cultures: Immunocytochemistry for Cell Phenotyping

Amanda Parker Struckhoff and Luis Del Valle

11. Transfection of Neuronal Cultures

Ilker Kudret Sariyer

12. Lentiviral Transduction of Neuronal Cells

Hassen S. Wollebo, Baheru Woldemichaele, and Martyn K. White

13. Compartmentalized Neuronal Cultures

Armine Darbinyan, Paul Pozniak, Nune Darbinian, Martyn K. White, and Kamel Khalili

14. Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates

George M. Smith, Yingpeng Liu, and Jee W. Hong

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