Nonviral Vectors for Gene Therapy: Methods and Protocols / Edition 1

Nonviral Vectors for Gene Therapy: Methods and Protocols / Edition 1

by Mark A. Findeis
     
 

The purpose of this volume of Methods in Molecular Medicine is to set forth examples of the great variety of techniques and applications that are now emerging in the field of nonviral gene therapy. The book emphasizes not only specific approaches to gene delivery but, in particular, the best current me- ods to prepare, handle, and characterize gene delivery agents.

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Overview

The purpose of this volume of Methods in Molecular Medicine is to set forth examples of the great variety of techniques and applications that are now emerging in the field of nonviral gene therapy. The book emphasizes not only specific approaches to gene delivery but, in particular, the best current me- ods to prepare, handle, and characterize gene delivery agents. These topics are of very broad importance since gene therapy evolves from its mostly ac- emy-based experimental and clinical research to the ever increasing number of industry-driven programs directed toward commercial development. S- cessful introduction of nonviral gene therapy agents into the clinic should be expected to require rigorous manufacturing and analytical methods that readily meet the regulatory guidelines under which new drug candidates are reviewed for marketing approval. Exactly what those guidelines will prove to be c- tainly depends on the established guidelines for review of both biological and chemical therapeutics. Additionally, many new techniques are being devised and applied to gene therapy research; these techniques will be instrumental in developing and characterizing successful gene delivery agents. Nonviral Vectors for Gene Therapy: Methods and Prools has two main sections. To start with, there is a series of chapters on specific prools for the synthesis, characterization, and application of gene delivery agents. S- eral chapters address the topic of materials to bind with DNA to form the compact condensed phases that facilitate cellular delivery.

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Product Details

ISBN-13:
9781617371455
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/09/2010
Series:
Methods in Molecular Medicine Series, #65
Edition description:
Softcover reprint of hardcover 1st ed. 2001
Pages:
399
Product dimensions:
0.92(w) x 6.00(h) x 9.00(d)

Table of Contents

Synthesis of Polyampholyte Comb-Type Copolymers Consisting of a Poly(l-lysine) Backbone and Hyaluronic Acid Side Chains for DNA Carrier
Atsushi Maruyama and Yoshiyuki Takei

Cationic _-Helical Peptides for Gene Delivery into Cells
Takuro Niidome and Haruhiko Aoyagi

Supramolecular Self-Assembly of Poly(ethylene glycol)-Block-Poly (l-lysine) Dendrimer with Plasmid DNA
Joon Sig Choi and Jong Sang Park

Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery
Gert W. Bos, Daan J. A. Crommelin, and Wim E. Hennink

Stabilization of Polycation-DNA Complexes by Surface Modification with Hydrophilic Polymers
David Oupicky, Martin L. Read, and Thierry Bettinger

Use of Disulfide Cationic Lipids in Plasmid DNA Delivery
Fuxing Tang and Jeffrey A. Hughes

Interactions of Lipid-Oligonucleotide Conjugates with Low-Density Lipoprotein
Erik T. Rump, Erik A. L. Biessen, Theo J. C. van Berkel, and Martin K. Bijsterbosch

Coupling of Nuclear Localization Signals to Plasmid DNA
Carole Neves, Daniel Scherman, and Pierre Wils

Progress Toward a Synthetic Virus: A Multicomponent System for Liver-Directed DNA Delivery
Bo-Hua Zhong, George Y. Wu, and Catherine H. Wu

Characterization of Polyampholyte Comb-Type Copolymer DNA Carriers
Yoshiyuki Takei, Atsushi Maruyama, Toshihiro Akaike, and Nobuhiro Sato

Methods for Studying the Formation of Polycation-DNA Complexes and Properties Useful for Gene Delivery
Martin L. Read, Thierry Bettinger, and David Oupicky

Characterization of DNA Condensates by Atomic Force Microscopy
Ye Fang and Jan H. Hoh

Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitting
Rona S. Scott and Jean-Michel H. Vos

Systemic Delivery of Therapeutic Proteins by Intramuscular Injection of Plasmid DNA
Holly M. Hortonand Suezanne E. Parker

Local Delivery of Therapeutic Proteins by Intratumoral Injection of Plasmid DNA-Lipid Complexes
Holly M. Horton and Suezanne E. Parker

Nonviral DNA Delivery from Polymeric Systems
Lonnie D. Shea and David J. Mooney

Promotion of Duplex and Triplex DNA Formation by Polycation Comb-Type Copolymers
Hidetaka Torigoe and Atsushi Maruyama

Lyophilization of Nonviral Gene Delivery Systems
S. Dean Allison and Thomas J. Anchordoquy

Ultraviolet Absorption and Circular Dichroism Spectroscopy of Nonviral Gene Delivery Complexes
Chad S. Braun, Lisa A. Kueltzo, and C. Russell Middaugh

Characterization of Synthetic Gene Delivery Vectors by Infrared Spectroscopy
Sirirat Choosakoonkriang, Christopher M. Wiethoff, Lisa A. Kueltzo, and C. Russell Middaugh

Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry
Brian A. Lobo, Sheila A. Rogers, Christopher M. Wiethoff, Sirirat Choosakoonkriang, Susan Bogdanowich-Knipp, and C. Russell Middaugh

Light-Scattering Techniques for Characterization of Synthetic Gene Therapy Vectors
Christopher M. Wiethoff and C. Russell Middaugh

Renal Gene Therapy
Yeong-Hau H. Lien and Li-Wen Lai

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