Nucleic Acid and Peptide Aptamers: Methods and Protocols / Edition 1

Hardcover (Print)
Used and New from Other Sellers
Used and New from Other Sellers
from $34.65
Usually ships in 1-2 business days
(Save 78%)
Other sellers (Hardcover)
  • All (10) from $34.65   
  • New (3) from $117.56   
  • Used (7) from $34.65   


After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In "Nucleic Acid and Peptide Aptamers: Methods and Prools", expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology™ series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, "Nucleic Acid and Peptide Aptamers: Methods and Prools" will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.

Read More Show Less

Editorial Reviews

From the Publisher
From the reviews:

"The book ‘Nucleic Acids and Peptide Aptamers’ is a collection of detailed prools describing various aspects related to generating aptamers, characterizing them, and applying for particular purposes. … The list of references is a useful and timely source of further reading on the subject. … can be certainly recommended as an excellent handbook addressed to researchers and students in the molecular biology field." (Ryszard W. Adamiak and Mikolaj Olejniczak, Acta Biochimica Polonica, Vol. 56 (3), 2009)

Read More Show Less

Product Details

  • ISBN-13: 9781934115893
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 3/3/2009
  • Series: Methods in Molecular Biology Series, #535
  • Edition description: 2009
  • Edition number: 1
  • Pages: 408
  • Product dimensions: 7.70 (w) x 10.30 (h) x 1.00 (d)

Table of Contents

Part I.) Nucleic Acid Aptamers

1. Nucleic Acid Pool Preparation and Characterization

Shawn K. Piasecki, Bradley Hall, and Andrew D. Ellington

2. In vitro Selection of ssDNA Aptamers Using Biotinylated Target Proteins

Günter Mayer and Thomas Höver

3. Isolating Aptamers Using Capillary Electrophoresis-SELEX (CE-SELEX)

Renee Mosing and Michael T. Bowser

4. In vitro Selection of Allosteric Ribozymes

Nicolas Piganeau

5. Cell-Specific Aptamers for Targeted Therapies

Laura Cerchia, Paloma H. Giangrande, James O. McNamara, and Vittorio de Franciscis

6. Aptamers Targeting RNA Molecules

Marguerite Watrin, Eric Dausse, Isabelle Lebars, Bernard Rayner, Anthony Bugaut, and Jean-Jaques Toulmé

7. Fluorescence Correlation Spectroscopy (FCS) Based Characterization of Aptamer Ligand Interaction

Arne Werner and Uli Hahn

8. Structural Probing Techniques on Natural Aptamers

Catherine A. Wakeman and Wade C. Winkler

9. Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography

Andrea L. Edwards, Andrew D. Garst, and Robert T. Batey

10. Locked Nucleic Acid Aptamers

Jan Barciszewski, Michael Medgaard, Troels Koch, Jens Kurreck, and Volker A. Erdmann

11. Screening of Novel Inhibitors of HIV-1 Reverse Transcriptase with a Reporter Ribozyme Assay

Satoko Yamazaki and Michael Famulok

12. Aptamers as Artificial Gene Regulation Elements

Beatrix Suess and Julia E. Weigand

13. Aptamers and Biosensors

Thomas M. A. Gronewold

14. Nanoparticles/Dip Stick

Yi Lu, Juewen Liu, and Debapriya Mazumdar

15. In vivo Imaging of Oligonucleotidic Aptamers

Bertrand Tavitian, Frédéric Ducongé, Raphaël Boisgard and Frédéric Dollé Tavitian

Part II.) Peptide Aptamers

16. Selection of Peptides Interfering with Protein-Protein Interaction

Annette Gaida, Urs B. Hagemann, Dinah Mattay, Christina Räuber, Kristian M. Müller, and Katja M. Arndt

17. In Vitro Selection of Protein and Peptide Libraries Using mRNA Display

Terry T. Takahashi and Richard W. Roberts

18. Ligand-Regulated Peptide Aptamers

Russell A. Miller

19. Isolation of Peptide Aptamers to Target Protein Function

Luisa Lopez-Ochoa, Tara E. Nash, Jorge Ramirez-Prado, and Linda Hanley-Bowdoin

20. Microbodies™

Hans-Ulrich Schmoldt, Matin Daneschdar, Harald Kolmar, and Michael Blind

21. Peptide Aptamers for Small Molecule Drug Discovery

Carine Bardou, Christophe Borie, Marc Bickle, Brian B. Rudkin, and Pierre Colas

22. Synthesis and Application of Peptides as Drug Carriers

Robert Rennert, Ines Neundorf, and Annette G. Beck-Sickinger

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)