Nucleic Acid and Peptide Aptamers: Methods and Protocols / Edition 1

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Overview

After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In "Nucleic Acid and Peptide Aptamers: Methods and Prools", expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology™ series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, "Nucleic Acid and Peptide Aptamers: Methods and Prools" will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.

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Editorial Reviews

From the Publisher
From the reviews:

"The book ‘Nucleic Acids and Peptide Aptamers’ is a collection of detailed prools describing various aspects related to generating aptamers, characterizing them, and applying for particular purposes. … The list of references is a useful and timely source of further reading on the subject. … can be certainly recommended as an excellent handbook addressed to researchers and students in the molecular biology field." (Ryszard W. Adamiak and Mikolaj Olejniczak, Acta Biochimica Polonica, Vol. 56 (3), 2009)

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Product Details

  • ISBN-13: 9781934115893
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 3/3/2009
  • Series: Methods in Molecular Biology Series , #535
  • Edition description: 2009
  • Edition number: 1
  • Pages: 408
  • Product dimensions: 7.70 (w) x 10.30 (h) x 1.00 (d)

Table of Contents

Part I.) Nucleic Acid Aptamers

1. Nucleic Acid Pool Preparation and Characterization

Shawn K. Piasecki, Bradley Hall, and Andrew D. Ellington

2. In vitro Selection of ssDNA Aptamers Using Biotinylated Target Proteins

Günter Mayer and Thomas Höver

3. Isolating Aptamers Using Capillary Electrophoresis-SELEX (CE-SELEX)

Renee Mosing and Michael T. Bowser

4. In vitro Selection of Allosteric Ribozymes

Nicolas Piganeau

5. Cell-Specific Aptamers for Targeted Therapies

Laura Cerchia, Paloma H. Giangrande, James O. McNamara, and Vittorio de Franciscis

6. Aptamers Targeting RNA Molecules

Marguerite Watrin, Eric Dausse, Isabelle Lebars, Bernard Rayner, Anthony Bugaut, and Jean-Jaques Toulmé

7. Fluorescence Correlation Spectroscopy (FCS) Based Characterization of Aptamer Ligand Interaction

Arne Werner and Uli Hahn

8. Structural Probing Techniques on Natural Aptamers

Catherine A. Wakeman and Wade C. Winkler

9. Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography

Andrea L. Edwards, Andrew D. Garst, and Robert T. Batey

10. Locked Nucleic Acid Aptamers

Jan Barciszewski, Michael Medgaard, Troels Koch, Jens Kurreck, and Volker A. Erdmann

11. Screening of Novel Inhibitors of HIV-1 Reverse Transcriptase with a Reporter Ribozyme Assay

Satoko Yamazaki and Michael Famulok

12. Aptamers as Artificial Gene Regulation Elements

Beatrix Suess and Julia E. Weigand

13. Aptamers and Biosensors

Thomas M. A. Gronewold

14. Nanoparticles/Dip Stick

Yi Lu, Juewen Liu, and Debapriya Mazumdar

15. In vivo Imaging of Oligonucleotidic Aptamers

Bertrand Tavitian, Frédéric Ducongé, Raphaël Boisgard and Frédéric Dollé Tavitian

Part II.) Peptide Aptamers

16. Selection of Peptides Interfering with Protein-Protein Interaction

Annette Gaida, Urs B. Hagemann, Dinah Mattay, Christina Räuber, Kristian M. Müller, and Katja M. Arndt

17. In Vitro Selection of Protein and Peptide Libraries Using mRNA Display

Terry T. Takahashi and Richard W. Roberts

18. Ligand-Regulated Peptide Aptamers

Russell A. Miller

19. Isolation of Peptide Aptamers to Target Protein Function

Luisa Lopez-Ochoa, Tara E. Nash, Jorge Ramirez-Prado, and Linda Hanley-Bowdoin

20. Microbodies™

Hans-Ulrich Schmoldt, Matin Daneschdar, Harald Kolmar, and Michael Blind

21. Peptide Aptamers for Small Molecule Drug Discovery

Carine Bardou, Christophe Borie, Marc Bickle, Brian B. Rudkin, and Pierre Colas

22. Synthesis and Application of Peptides as Drug Carriers

Robert Rennert, Ines Neundorf, and Annette G. Beck-Sickinger

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