Nucleic Acids Isolation Methods is devoted to preparation of genomic DNA, plasmid DNA, viral nucleic acids, and RNA. Emphasis is also put on stabilization of nucleic acids prior to isolation, scale-up of plasmid isolation, and DNA cleanup from gels, PCR, sequencing, etc. A number of specific medical and forensic applications are detailed such as preparation of pathogen nucleic acids for real-time PCR analysis, extraction of DNA from paraffin embedded bone marrow trephine biopsies, and isolation of nucleic acids from tissue, blood, semen, hair, and saliva for DNA fingerprints, RFLP, and PCR analyses. This book contains 10 state-of-the-art review chapters written by 20 renowned experts from leading research institutions, industry and a criminal investigation department. Detailed description of methods as well as their theoretical background makes this volume an essential reference for specialists in academia, research institutions, and industry and also a valuable manual for newcomers to this field.
Key Features
- Most up-to-date reference summarizing latest methods on nucleic acids isolation methods.
- Covers nucleic acids isolation methods and their biochemical, biological, forensic and medical applications.
- 10 state-of-the-art chapters written by research experts and market leaders involved in the production of commercial kits for nucleic acid isolation and PCR diagnostics.
- Hundreds of bibliographic citations, illustrations, figures, tables and equations.
- A perfect reference source for students, researchers and specialists working in the fields of nucleic acids, microbiology, biotechnology, genetics, genome science and engineering, biochemistry, medical sciences, biology, biomedical engineering and forensic science.
