Description: This Methods in Enzymology volume is a much needed compendium on methods applicable to assays of nitrogen monoxide (NO), especially assays of biological activities, and studies of NO donors, and oxidant and antioxidant activities of NO. Also included are study methods for regulation of NO and studies of NO at the molecular, cellular, tissue, and organ levels and in expression systems and clinical situations.
Purpose: This volume is meant to update methods of use in this rapidly advancing field, beyond the reviews in the earlier volumes 268, 269, and 301. Since NO encompasses so many research directions, this volume should serve to update researchers in many areas.
Audience: The information will be very useful for biochemists or clinicians, students or experienced investigators. Since this area is rapidly growing, biochemists in general should find the content of potential value.
Features: This volume has the attractive Methods in Enzymology format with sufficient illustrations, references, and subject index as well as very clear and concise writing.
Assessment: This volume is highly recommended for anyone with an interest in studies of NO. Since applications in so many different areas are covered and since the roles of NO are so varied and its presence ubiquitous, this volume should be of general usefulness for investigators in many different fields. A general introduction to the biological activities of NO would have been useful for novices, since this area is newer than the general oxidant/antioxidant area.