Pain Research / Edition 1

Pain Research / Edition 1

by Z. David Luo
     
 

Despite significant advances in molecular biology techniques and in our understanding of the physiology and the behavioral pharmacology of pain transduction, effective, target-specific therapeutic agents for chronic pain are still lacking. In Pain Research: Methods and Protocols, leading researchers who have first-hand experience describe, in step-by-step detail,… See more details below

Overview

Despite significant advances in molecular biology techniques and in our understanding of the physiology and the behavioral pharmacology of pain transduction, effective, target-specific therapeutic agents for chronic pain are still lacking. In Pain Research: Methods and Protocols, leading researchers who have first-hand experience describe, in step-by-step detail, diverse and novel techniques for dissecting the molecular mechanisms of pain transduction. These readily reproducible methods employ a variety of multidisciplinary approaches ranging from animal pain models and single neutron selection to in vitro single-cell mRNA amplification. The collection includes not only standard, cutting-edge methods, but also novel techniques only recently applied to pain research. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Versatile and easy-to-use, Pain Research: Methods and Protocols offers today's pain researchers in academic and pharmaceutical laboratories powerful tools to unravel the cellular and molecular complexity of pain transduction and set the stage for the next generation of pain medications.

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Product Details

ISBN-13:
9781588291035
Publisher:
Springer-Verlag New York, LLC
Publication date:
05/15/2004
Series:
Methods in Molecular Medicine Series
Pages:
316
Product dimensions:
9.21(w) x 6.14(h) x 0.75(d)

Meet the Author

Table of Contents

Mechanistic Dissection of Pain: From DNA to Animal Models

Z. David Luo

Assessment of Acute Thermal Nociception in Laboratory Animals

Jeffrey W. Allen and Tony L. Yaksh

Tissue Injury Models of Persistent Nociception in Rats

Jeffrey W. Allen and Tony L. Yaksh

Segmental Spinal Nerve Ligation Model of Neuropathic Pain

Jin Mo Chung, Hee Kee Kim, and Kyungsoon Chung

Partial Sciatic Nerve Transection

Thies Lindenlaub and Claudia Sommer

Modeling Diabetic Sensory Neuropathy in Rats

Nigel A. Calcutt

Sciatic Inflammatory Neuropathy in the Rat: Surgical Procedures, Induction of Inflammation, and Behavioral Testing

Erin D. Milligan, Steven F. Maier, and Linda R. Watkins

A Rat Pain Model of Vincristine-Induced Neuropathy

Emiliano S. Higuera and Z. David Luo

Loop Dialysis Catheter: A Technology for Chronic Spinal Dialysis in a Freely Moving Rat

Martin Marsala

Intrathecal Catheterization and Drug Delivery in the Rat

Shelle A. Malkmus and Tony L. Yaksh

Trigeminal Neuronal Recording in Animal Models of Orofacial Pain

Koichi Iwata, Yuji Masuda, and Ke Ren

In Vivo Electrophysiology of Dorsal-Horn Neurons

Louise C. Stanfa and Anthony H. Dickenson

Single-Fiber Recording: In Vivo and In Vitro Preparations

Maria Schäfers and David Cain

Anatomical Identification of Neurons Responsive to Nociceptive Stimuli

Luc Jasmin and Peter T. Ohara

Isolation and Culture of Sensory Neurons From the Dorsal-Root Ganglia of Embryonic or Adult Rats

Thomas H. Burkey, Cynthia M. Hingtgen, and Michael R. Vasko

Primary Cultures of Neonatal Rat Spinal Cord

Virginia S. Seybold and Lia G. Abrahams

Single-Cell Laser Capture Microdissection and RNA Amplification

Fredrik Kamme, Jessica Zhu, Lin Luo, Jingxue Yu, Da-Thao Tran, Bernhard Meurers, Anton Bittner, Karin Westlund, Susan Carlton, and Jackson Wan

Semi-Quantitative Real-Time PCR for Pain Research

Hong Qing Guo and Sandra R. Chaplan

Functional Genomic Analysis in Pain Research Using Hybridization Arrays

Stephen J. Walker, Travis J. Worst, Willard M. Freeman, and Kent E. Vrana

Generation of Transgenic Mice

Guoping Feng, Jing Lu, and Jimmy Gross

Knockout Mouse Models in Pain Research

Andrée Dierich and Brigitte L. Kieffer

Index

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