PCR in Bioanalysis / Edition 1

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Hardcover New 0896034976 Book is brand new and in the plastic wrap, ships quickly. 100% of the proceeds help send books to schools and libraries in developing countries through ... the International Book Project. 100% of proceeds help send books overseas to schools and libraries throughout the developing world. Read more Show Less

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1998 Hardcover 8vo, hardcover, no dj, as issued. New. Bright, crisp & clean, unread. 281 pp.

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PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, and the detection of tumor contamination of stem cells.

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Editorial Reviews

Twenty-four contributions cover the use of the polymerase chain reaction in the diagnosis of infectious disease, the detection of amplified or deleted DNA sequences and circulating tumor cells, the quantitative measurement of mRNA expression, the identification and cloning of differentially expressed genes, and the process of screening for chain-terminating mutations. Of interest to pathologists, microbiologists, and molecular and cell biologists. Annotation c. by Book News, Inc., Portland, Or.
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Product Details

  • ISBN-13: 9780896034976
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 1/5/1998
  • Series: Methods in Molecular Biology Series, #92
  • Edition description: 1998
  • Edition number: 1
  • Pages: 296
  • Product dimensions: 9.21 (w) x 6.14 (h) x 0.69 (d)

Table of Contents

Application of Nucleic Acid Amplification in Clinical Microbiology
Gorm Lisby

Detection of Rodent Parvoviruses by PCR
David G. Besselsen

The SURF Technique: Selective Genetic Analysis of Microscopic Tissue Heterogeneity
Darryl Shibata

Prenatal and Presymptomatic Diagnosis of Marfan Syndrome Using Fluorescence PCR and an Automated Sequencer
Mei Wang and Maurice Godfrey.

Measurement of TNF and iNOS mRNA Using cDNA-Equalized Reverse Transcriptase PCR
Jay K. Kolls and Jianming Xie

PCR Diagnosis of the Bovine Immunodeficiency-Like Virus
David L. Suarez and Cecelia A. Whetstone

PCR Analysis for the Identification of Porcine Reproductive and Respiratory Syndrome Virus in Boar Semen
Jane Christopher-Hennings and Eric A. Nelson

Quantitative and Discriminative Detection of Individual HIV-mRNA Subspecies by an RNase Mapping Assay
Catherine Mary, Hideo Akaoka, and Bernard Verrier

Detection of the Heat-Labile Toxin Coding Gene (LT-Gene) of Enterotoxigenic Escherichia coli
Séamus Fanning, Deirdre O'Meara, Lesley Cotter, John O'Mullane, and Bartley Cryan

Motif-Dependent Polymerase Chain Reaction (PCR): DNA Fingerprinting Entererotoxigenic Escherichia coli
Séamus Fanning, Deirdre O'Meara, Lesley Cotter, Paddy Greer, and Bartley Cryan

In Vitro Transcription/Translation Analysis for the Identification of Translation-Terminating Mutations
Michael C. Luce, Cameron G. Binnie, Lauren N. W. Kam-Morgan, and Matthew C. Cayouette

A Single-Tube Nested RT-PCR for the Detection of Ross River Virus
Loryn Sellner

Application of PCR to Transgenic Plants
Michael Wassenegger

Detection of Hepatitis C Virus RNA by Semiquantitative Reverse-Transcription PCR
Anderson S. Gaweco and David H. Van Thiel

Detection of Legionella Species in Bronchial Fluid by PCR

Quantitative Measurement of mRNA Expression by Competitive RT-PCR
Joe O'Connell, Triona Goode, and Fergus Shanahan

PCR Detection of Toxoplasma gondii in Human Fetal Tissues
Tama's Tóth, István Sziller, and Zoltán Papp

Rapid Diagnosis of Pulmonary Tuberculosis Using Roche AMPLICORTM Mycobacterium tuberculosis PCR Test
Richard F. D'Amato and Albert Miller

The Use of Flow Cytometry and RT-PCR in the Detection of Circulating PSA-Positive Cells in Prostate Cancer
Emma J. Fadlon and Freddie C. Hamdy

Detection of Chlamydia trachomatis and Trichomonas vaginalis in the Vaginal Introitus, Posterior Vagina, and Endocervix by Polymerase Chain Reaction
Jan Jeremias, Vera Tolbert, and Steven S. Witkin

Detection and Isolation of Differentially Expressed Genes by PCR
John M. Abraham

Detection of Transgene Integrants and Homologous Recombinants in Mice by Polymerase Chain Reaction
Kristin M. Abraham, Nancy S. Longo, and Judith A. Hewitt

Direct Analysis for Familial Adenomatous Polyposis Mutations
Steven M. Powell

PCR Fingerprinting for Detection of Deleted or Amplified Sequences in Human Cancer
Takashi Kohno and Jun Yokota


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