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Doody's Review ServiceReviewer: Omer Iqbal, MD, FACC, FESC (Loyola University Medical Center)
Description: This book describes recently developed tools such as PCR cloning and sequencing, expression, copy number or methylation profile analysis, DNA fingerprinting, diagnostics, protein engineering interaction screening, and other important techniques crucial for all PCR methods.
Purpose: The purpose is to provide scientists with latest tools necessary for PCR methods.
Audience: Students and researchers interested in learning latest methods in PCR will find this book extremely useful.
Features: The book is divided into five parts. Part I covers PCR husbandry and includes an interesting chapter on the setup of a PCR laboratory. Part II covers cloning and sequencing, part III, expression, epigenetic and copy number analysis, part IV, probes and assays, and part V, engineering and screening. Each chapter uses the same format: an introduction, lists of necessary reagents and materials, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Assessment: This book presents cutting-edge methodologies crucial for all PCR methods. Given the rapid advances in the field of PCR methods, this third edition is essential and justifies replacement of the previous edition.