Peptide Analysis Protocols / Edition 1by Ben M. Dunn, Michael W. Pennington, David C. Dunn
Pub. Date: 11/07/1994
Publisher: Springer-Verlag New York, LLC
The authors describe the procedures in a practical, step-by-step fashion
In this second volume of a two-volume set, state-of-the-art methods are given for the analysis of peptides. Leading authorities provide reproducible protocols for peptide analysis using HPLC, capillary zone electrophoresis, mass spectrometry, UV and fluorescence spectroscopy, to name a few.
The authors describe the procedures in a practical, step-by-step fashion suitable for both experts or novices. Chapters also include a helpful Notes section offering troubleshooting tips, alternative procedures, details on substituting unavailable materials, and informative explanations about why certain steps are necessary. These extra measures help to guarantee the successful outcome of your experiments.
Dunn and Pennington's Peptide Analysis Protocols offers a comprehensive collection of standard, readily reproducible methods for many procedures that will enable scientists in every area of biochemical and biological research to analyze those peptides that have been synthesized in the laboratory.
Table of ContentsGel-Filtration Chromatography, Daniel M. Bollag. Ion-Exchange Chromatography, Daniel M. Bollag. Reversed-Phase HPLC: Analytical Procedure, Udo Nirenberg. Reversed-Phase High-Performance Liquid Chromatography: A Semipreparative Methodology, Michael E. Byrnes. Applications of Strong Cation-Exchange (SCX)-HPLC in Synthetic Peptide Analysis, Dan L. Crimmins. Principles and Practice of Peptide Analysis with Capillary Zone Electrophoresis, Thomas E. Wheat. Fast Atom Bombardment Mass Spectrometric Characterization of Peptides, P. R. Das and B. N. Pramanik. Sequence Analysis of Peptide Resins from Boc/Benzyl Solid-Phase Synthesis, Jan Pohl. NMR Spectroscopy of Peptides and Proteins, Mark G. Hinds and Raymond S. Norton. Techniques for Conjugation of Synthetic Peptides to Carrier Molecules, J. Mark Carter. Epitope Prediction Methods, J. Mark Carter. Epitope Mapping of a Protein Using the Geysen (PEPSCAN) Procedure, J. Mark Carter. Analysis of Proteinase Specificity by Studies of Peptide Substrates:The Use of UV and Fluorescence Spectroscopy to Quantitate Rates of Enzymatic Cleavage, Ben M. Dunn, Paula E. Scarborough, Ruth Davenport, and Wieslaw Swietnicki. Synthesis of Recombinant Peptides, Gino Van Heeke, Jay S. Stout, and Fred W. Wagner. De Novo Design of Proteins: Template-Assembled Synthetic Proteins (TASP), Gabriele Tuchscherer, Verena Steiner, Karl-Heinz Altmann, and Manfred Mutter. Chemical Synthesis of the Aspartic Proteinase from Human Immunodeficiency Virus (HIV), Paul D. Hoeprich, Jr. Multiple and Combinatorial Peptide Synthesis: Chemical Development and Biological Applications, Philip C. Andrews, Daniele M. Leonard, Wayne L. Cody, and Tomi K. Sawyer. Index.
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