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Peptide Synthesis Protocols / Edition 1

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Overview

In Peptide Synthesis Prools leading authorities assemble in one volume a broad range of state-of-the-art methods for the preparation, purification, and synthesis of peptides. These powerful and useful prools contain many innovations not previously described, including a compilation of chemical modification procedures and peptide synthesis improvements.

The authors describe each procedure in a practical, step-by-step fashion suitable for both the skilled and novice researcher. They also provide troubleshooting tips, alternative ways of doing things, and informative explanations about why certain steps are necessary—aids not usually found in standard journal recipes—all designed to guarantee a significant difference in the outcome of your experiments.

Pennington and Dunn's Peptide Synthesis Prools offers a comprehensive collection of standardized, readily reproducible methods for many critical procedures that will enable scientists in every area of biochemical and biological research to prepare, purify, characterize, and use peptides effectively.

Solvents for solid-phase peptide synthesis/peptide synthesis via fragment condensation/Fmoc group removal methods/etc.

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Editorial Reviews

From the Publisher
...a useful laboratory manual-Trends in Biotechnology
Booknews
A reference that assembles a broad range of state-of-the-art methods for the preparation, purification, and characterization of peptides. The protocols contain many innovations not previously described, including a compilation of chemical modification procedures and peptide synthesis improvements. The authors provide step-by-step descriptions of each procedure, as well as troubleshooting tips, alternative procedures, and explanations about why certain steps are necessary. Plastic comb-binding. Annotation c. Book News, Inc., Portland, OR booknews.com
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Product Details

  • ISBN-13: 9780896032736
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/7/1994
  • Series: Methods in Molecular Biology Series , #35
  • Edition description: 1995
  • Edition number: 1
  • Pages: 333
  • Product dimensions: 9.21 (w) x 6.14 (h) x 0.70 (d)

Table of Contents

Procedures to Improve Difficult Couplings, Michael W. Pennington and Michael E. Byrnes. Methods for Removing the Fmoc Group, Gregg B. Fields. Solvents for Solid-Phase Peptide Synthesis, Cynthia G. Fields and Gregg B. Fields. HF Cleavage and Deprotection Procedures for Peptides Synthesized Using a Boc/Bzl Strategy, Michael W. Pennington. Acid Cleavage/Deprotection in Fmoc/tBu-Solid Phase Peptide Synthesis, Fritz Dick. Bromoacetylated Synthetic Peptides: Starting Materials for Cyclic Peptides, Peptomer, and Peptide Conjugates. Frank A. Robey. Formation of Disulfide Bonds in Synthetic Peptides and Proteins, David Andreau, Fernando Albericio, Núria A. Solé, Mark C. Munson, Marc Ferrer, and George Barany. Site-Specific Chemical Modification Procedures, Michael W. Pennington. Synthesis of Phosphopeptides Containing O-Phosphoserine and O-Phosphothreonine, Anatol Arendt and Paul A. Hargrave. Solid-Phase Synthesis of Phosphorylated Tyr-Peptides by 'Phosphite Triester' Phosphorylation, Michael W. Pennington. Design of Novel Synthetic Peptides Including Cyclic Conformationally and Topographically Constrained Analogs, Victor J. Hruby and G. Gregg Bonner. Solid-Phase Synthesis of Peptides Containing the CH2NH Reduced Bond Surrogate, Michael W. Pennington. Approaches to the Asymmetric Synthesis of Unusual Amino Acids, Victor J. Hruby and Xinhua Qian. Synthesis of Fully Protected Peptide Fragments, Monika Mergler. Peptide Synthesis via Fragment Condensation, Rolf Nyfeler. Index.

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