Pharmacogenomics: Methods and Protocols / Edition 1

by Federico Innocenti

Overview

Part I. History and Overview Pharmacogenomics: Historical Perspective and Current Status Werner Kalow Part II. Functional Analysis of Gene Variation Transfection Assays With Allele-Specific Constructs: Functional Analysis of UDP-Glucuronosyltransferase Variants Hideto Jinno, Nobumitsu Hanioka, Toshiko Tanaka-Kagawa, Yoshiro Saito, Shogo Ozawa, and Jun-ichi Sawada Snapshot of the Allele-Specific Variation in Human Gene Expression Hai Yan Genome-Wide Analysis of Allele-Specific Gene Expression Using Oligo Microarrays Maxwell P. Lee HaploChIP: An In Vivo Assay Julian Charles Knight Part III. Genotyping Techniques Aspects Influencing Genotyping Method Selection Peter Imle Denaturing High-Performance Liquid Chromatography for Mutation Detection and Genotyping Donna Lee Fackenthal, Pei Xian Chen, and Soma Das Pyrosequencing of Clinically Relevant Polymorphisms Sharon Marsh, Cristi R. King, Adam A. Garsa, and Howard L. McLeod Kinetic Fluorescence-Quenching Detection Assay for Allele Frequency Estimation Ming Xiao and Pui-Yan Kwok Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Matthias Wjst and Dirk van den Boom Fluorescence-Based Fragment Size Analysis Peter Imle Single-Nucleotide Polymorphism Genotyping in DNA Pools Ian Craig, Emma Meaburn, Lee Butcher, Linzy Hill, and Robert Plomin TaqMan Genotyping of Insertion/Deletion Polymorphisms Renato Robledo, William R. Beggs, and Patrick K. Bender Part IV. Management of Pharmacogenomic Information PharmGKB: The Pharmacogenetics and Pharmacogenomics Knowledge Base Caroline F. Thorn, Teri E. Klein, and Russ B. Altman Systems for the Management of Pharmacogenomic Information Alexander Sturn, Michael Maurer, Robert Molidor, and ZlatkoTrajanoski Index

Product Details

ISBN-13:	9781617375712
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/19/2010
Series:	Methods in Molecular Biology , #311
Edition description:	Softcover reprint of hardcover 1st ed. 2005
Pages:	214
Product dimensions:	5.98(w) x 9.02(h) x 0.02(d)

Table of Contents

History and Overview.- Pharmacogenomics.- Functional Analysis of Gene Variation.- Transfection Assays With Allele-Specific Constructs.- Snapshot of the Allele-Specific Variation in Human Gene Expression.- Genome-Wide Analysis of Allele-Specific Gene Expression Using Oligo Microarrays.- HaploChIP.- Genotyping Techniques.- Aspects Influencing Genotyping Method Selection.- Denaturing High-Performance Liquid Chromatography for Mutation Detection and Genotyping.- Pyrosequencing of Clinically Relevant Polymorphisms.- Kinetic Fluorescence-Quenching Detection Assay for Allele Frequency Estimation.- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.- Fluorescence-Based Fragment Size Analysis.- Single-Nucleotide Polymorphism Genotyping in DNA Pools.- TaqMan Genotyping of Insertion/Deletion Polymorphisms.- Management of Pharmacogenomic Information.- PharmGKB.- Systems for the Management of Pharmacogenomic Information.