Pharmacogenomics: Methods and Protocols

by Federico Innocenti (Editor), Ron H.N. van Schaik (Editor)

Overview

Much of the research in pharmacogenomics aims at providing drugs made to match individuals' genetic makeup for greater efficacy and safety. In this collection of 15 papers contributors describe their work in both the newest and most common technologies, providing readily reproducible methods for assessing a polymorphism, evaluating genotyping platforms, and managing information. Along with a history and overview of pharmacogenomics, topics of individual papers include transfection assays with allele-specific constructs, a snapshot of the allele-specific variation in human gene expression, oligo microarrays as analysis tools, genotyping methods selection, high- performance liquid chromotography, pyrosequencing of clinically relevant polymorphisms, advanced mass spectrometry, florescence-based fragment size analysis, and single-nucleotide polymorphism genotyping in DNA pools. Annotation ©2005 Book News, Inc., Portland, OR

Product Details

ISBN-13:	9781627034340
Publisher:	Springer-Verlag New York, LLC
Publication date:	07/04/2013
Series:	Methods in Molecular Biology , #1015
Edition description:	2nd ed. 2013
Pages:	359
Product dimensions:	7.01(w) x 10.00(h) x 0.04(d)

Table of Contents

History and Overview.- Pharmacogenomics.- Functional Analysis of Gene Variation.- Transfection Assays With Allele-Specific Constructs.- Snapshot of the Allele-Specific Variation in Human Gene Expression.- Genome-Wide Analysis of Allele-Specific Gene Expression Using Oligo Microarrays.- HaploChIP.- Genotyping Techniques.- Aspects Influencing Genotyping Method Selection.- Denaturing High-Performance Liquid Chromatography for Mutation Detection and Genotyping.- Pyrosequencing of Clinically Relevant Polymorphisms.- Kinetic Fluorescence-Quenching Detection Assay for Allele Frequency Estimation.- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.- Fluorescence-Based Fragment Size Analysis.- Single-Nucleotide Polymorphism Genotyping in DNA Pools.- TaqMan Genotyping of Insertion/Deletion Polymorphisms.- Management of Pharmacogenomic Information.- PharmGKB.- Systems for the Management of Pharmacogenomic Information.