Pheromone Signaling: Methods and Protocols

Overview

Providing experimental methods and prools for performing pheromone research in a variety of organisms ranging from invertebrates to vertebrates, Pheromone Signaling: Methods and Prools covers a wide spectrum of experimental approaches necessary for handling pheromone molecules, measuring receptor response and neural activation, and analyzing behavioral output. A great deal of progress has been made in understanding the molecular mechanisms underlying pheromone action, largely due to the discovery of receptor ...

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Overview

Providing experimental methods and prools for performing pheromone research in a variety of organisms ranging from invertebrates to vertebrates, Pheromone Signaling: Methods and Prools covers a wide spectrum of experimental approaches necessary for handling pheromone molecules, measuring receptor response and neural activation, and analyzing behavioral output. A great deal of progress has been made in understanding the molecular mechanisms underlying pheromone action, largely due to the discovery of receptor genes, the advancement of imaging techniques, and key multi-disciplinary approaches including aspects of organic chemistry, biochemistry, molecular biology, electrophysiology, and behavioral science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Pheromone Signaling: Methods and Prools details the key methodologies utilized in laboratories all over the world, making them accessible to those who want to begin investigation in the area of pheromone research.

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Product Details

  • ISBN-13: 9781627036184
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 9/19/2013
  • Series: Methods in Molecular Biology Series , #1068
  • Edition description: 2013
  • Edition number: 1
  • Pages: 399
  • Product dimensions: 7.10 (w) x 10.10 (h) x 1.00 (d)

Table of Contents

Part I: Sampling, Chromatographic Purification, and Chemical Analysis of Pheromone Molecules

1. Female Moth Pheromones

Tetsu Ando

2. Pheromones in the Fruit Fly

Jacqueline Chin and Joanne Y. Yew

3. Analysis of Volatile Mouse Pheromones by Gas Chromatography Mass Spectrometry

Milos V. Novotny and Helena A. Soini

4. Murine Non-Volatile Pheromones: Isolation of Exocrine-Gland Secreting Peptide 1

Hiroko Kimoto and Kazushige Touhara

5. Chemical Analysis of Aquatic Pheromones in Fish

Michael Stewart, Cindy F. Baker, and Peter W. Sorensen

6. Analysis of Ascarosides from Caenorhabditis elegans Using Mass Spectrometry and NMR Spectroscopy

Xinxing Zhang, Jaime H. Noguez, Yue Zhou, and Rebecca A. Butcher

Part II: Approaches to Characterize Gene and Function of Pheromone Receptors

7. Identification of Chemosensory Receptor Genes from Vertebrate Genomes

Yoshihito Niimura

8. Functional Assays for Insect Olfactory Receptors in Xenopus Oocytes

Tatsuro Nakagawa and Kazushige Touhara

9. A Prool for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors

Sandeepa Dey, Senmiao Zhan, and Hiroaki Matsunami

10. Genetic Manipulation to Analyze Pheromone Responses: Knockouts of Multiple Receptor Genes

Tomohiro Ishii

Part III: Methods in Measuring Neural Responses to Pheromones

11. Electro-Antennogram and Single Sensillum Recording in Insect Antennae

Shannon B. Olsson and Bill S. Hansson

12. Calcium Imaging of Pheromone Responses in the Insect Antennal Lobe

Susy M. Kim and Jing W. Wang

13. Live Cell Calcium Imaging of Dissociated Vomeronasal Neurons

Angeldeep Kaur, Sandeepa Dey, and Lisa Stowers

14. Whole Mount Imaging of Responses in Mouse Vomeronasal Neurons

Pei Sabrina Xu and Timothy E. Holy

15. Calcium Imaging of Vomeronasal Organ Response Using Slice Preparations from Transgenic Mice Expressing G-CaMP2

C. Ron Yu

16. The Electrovomeronasogram: Field Potential Recordings in the Mouse Vomeronasal Organ

Trese Leinders-Zufall and Frank Zufall

17. Electrical Recordings from the Accessory Olfactory Bulb in VNO-AOB Ex Vivo Preparations

Julian P. Meeks and Timothy E. Holy

18. Pheromone-Induced Expression of Immediate Early Genes in the Mouse Vomeronasal Sensory System

Sachiko Haga-Yamanaka and Kazushige Touhara

Part IV: Prools in Analyzing Behavioral and Endocrine Changes Elicited by Pheromones

19. Insect Pheromone Behavior: Fruit Fly

Daisuke Yamamoto, Soh Kohatsu, and Masayuki Koganezawa

20. Quantitative Assessment of Pheromone-Induced Dauer Formation in Caenorhabditis elegans

Scott J. Neal, Kyuhyung Kim, and Piali Sengupta

21. Acute Behavioral Responses to Pheromones in C. elegans (Adult Behaviors: Attraction, Repulsion)

Heeun Jang and Cornelia I. Bargmann

22. Behavioral Analysis of Pheromones in Fish

Peter W. Sorensen

23. Analysis of Male Aggressive and Sexual Behavior in Mice

Takefumi Kikusui

24. Assessment of Urinary Pheromone Discrimination, Partner Preference, and Mating Behaviors in Female Mice

Olivier Brock, Julie Bakker, and Michael J. Baum

25. Assessing Postpartum Maternal Care, Alloparental Behavior, and Infanticide in Mice: With Notes on Chemosensory Influences

Kumi O. Kuroda and Yousuke Tsuneoka

26. Testing Smell When It is Really Vital: Behavioral Assays of Social Odors in the Neonatal Mouse

Benoist Schaal, Syrina Al Aïn, and Bruno Patris

27. An Assay for Human Chemosignals

Idan Frumin and Noam Sobel

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