Phospholipid Signaling Protocols

Overview

This wide-ranging collection of state-of-the-art techniques for the essay of phospholipid-derived second messengers allows the identification and quantification of signal pathway activation. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomylinase and associated metabolites. Additional protocols are provided for the assay of phosphoinositide kinase activity and for the separation and identification of phospholipids, diacylglycerol, sphingolipids, and fatty acid metabolites. ...
See more details below
Paperback (Softcover reprint of the original 1st ed. 1998)
$117.95
BN.com price
(Save 15%)$139.00 List Price
Other sellers (Paperback)
  • All (6) from $118.66   
  • New (5) from $118.66   
  • Used (1) from $118.66   
Sending request ...

Overview

This wide-ranging collection of state-of-the-art techniques for the essay of phospholipid-derived second messengers allows the identification and quantification of signal pathway activation. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomylinase and associated metabolites. Additional protocols are provided for the assay of phosphoinositide kinase activity and for the separation and identification of phospholipids, diacylglycerol, sphingolipids, and fatty acid metabolites. Detailed, step-by-step instructions make this book suitable for both the newcomer and the experienced investigator.

The book contains black-and-white illustrations.

Read More Show Less

Editorial Reviews

Booknews
Contributors detail techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites. There is also extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA. Annotation c. by Book News, Inc., Portland, Or.
From the Publisher
". . . the detailed information provided in Phospholipid Signaling Prools makes it a welcome addition to an academic library that serves scientists actively working in the area of second messenger and membrane lipid chemistry. It is well representative of the previous volumes in the Methods in Molecular Biology series, and therefore is a worthwhile acquisition."-E-Streams

". . .this book is an extremely valuable resource for methodology in studying the molecular species of key phospholipid signaling molecules, as well as the enzymes that produce or degrade them. I personally have used several prools in this book, and have not had any trouble adapting the methods to my own applications."-The Quarterly Review of Biology

"...gives an excellent overview of the most common techniques in use as well in biochemistry and molecular biology...The editor is to be congratulated for putting these diverse, very important prools together..."-Cell Biology International 2000

"...does a very good job of covering what is a fairly diverse field and the prools described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signaling molecules."-Molecular Biotechnology

"...does a very good job covering what is a fairly diverse field and the prools described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signalling molecules." - Molecular Biotechnology

Read More Show Less

Product Details

  • ISBN-13: 9781489942609
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 4/30/2014
  • Series: Methods in Molecular Biology Series
  • Edition description: Softcover reprint of the original 1st ed. 1998
  • Pages: 380
  • Product dimensions: 6.00 (w) x 9.00 (h) x 0.82 (d)

Table of Contents

Part I. Monitoring of Activation of Phospholipid-Derived Cell Signaling Pathways

Phosphoinositidase C Activation Assay I: Cell Labeling, Stimulation, and Recovery of Cellular [3H] Phosphoinositides and [3H] Phosphoinositols

Ian M. Bird

Phosphoinositidase C Activation Assay II: Simple Analysis of Recovered Cellular Phosphoinositides and Phosphoinositols

Ian M. Bird

Phosphoinositidase C Activation Assay III: HPLC Analysis of Cellular Phosphoinositides and Phosphoinositols

Ian M. Bird

Measurement of Phosphoinositols and Phosphoinositides Using Radio High-Performance Liquid Chromatography Flow Detection

Lubo Zhang and Iain L. O. Buxton

Preparation of [3H]Phosphoinositol Standards and Conversion of [3H] Phosphoinositides to [3H] Phosphoinositols

Ian M. Bird

Inositol 1,4,5-Trisphosphate Mass Assay

Lubo Zhang

Measurement of Cellular Diacylglycerol Content

Wendy Bollinger Bollag and Richard D. Griner

Phosphatidylinositol 4-Kinases: Assays and Product Analysis

Rachel E. Meyers and Lewis C. Cantley

Phosphatidylinositol(3,4,5)Trisphosphate (Ptdins(3,4,5)P3) Mass Measurement Using a Radioligand Displacement Assay

Jeroen van der Kaay, Pete J. Cullen, and C. Peter Downes

Detection of Phosphatidylinositol-4-Phosphate 5-Kinase Activity Using Thin-Layer Chromatography

Gregory J. Parker, Joost C. Loijens, and Richard A. Anderson

Determination of Phospholipase C- or Phospholipase D-Catalyzed Phosphatidylcholine Hydrolysis

Michael J. O. Wakelam and Trevor R. Pettitt

Measurement of Phospholipase D Activity

Wendy Bollinger Bollag

Monitoring of Phospholipase A2 Activation in Cultured Cells Using Tritiated Arachidonic Acid

Sandie I. Briand, Sylvie G. Bernier, and GaƩtan Guillemette

Assay of Cellular Diacylglycerol and Monoacylglycerol Lipases

Bryan A. Wolf

Isotopic Efflux Studies as Indices of Phospholipase Activation

Jacob Vadakekalam and Stewart Metz

Extraction and Measurements of Prostanoids and Leukotrienes by Radioimmunoassays

Bruno Battistini, Serge Picard, Pierre Borgeat, and Pierre Sirois

Measurement of Prostanoids, Leukotrienes, and Isoprostanes By Enzyme Immunoassays

Bruno Battistini, Serge Picard, Pierre Borgeat, and Pierre Sirois

Measurements of Arachidonic Acid Metabolites Derived from the Lipoxygenase Pathways By High-Pressure Liquid Chromatography

Pierre Borgeat, Serge Picard, Bruno Battistini, and Pierre Sirois

Measurement of Sphingomyelin and Ceramide Cellular Levels After Sphingomyelinase-Mediated Sphingomyelin Hydrolysis

Pino Santana, Luisa F. Fanjul, and C. M. Ruiz de Galarreta

Sphingomyelin and Ceramide Mass Assay

Pino Santana, C. M. Ruiz de Galarreta, and Luisa F. Fanjul

Sphingosine Kinase: Assay and Product Analysis

Ana Olivera and Sarah Spiegel

Analytical Methods and Steps to Sample Preparation For Determination of Molecular Species of Fatty Acids

Sanda Clejan

HPLC Analytical Methods for the Separation of Molecular Species of Fatty Acids In Diacylglycerol and Cellular Phospholipids

Sanda Clejan

Analysis of Molecular Species of Cellular Sphingomyelins and Ceramides

Sanda Clejan

Part II. General methods for monitoring changes in levels of key signaling pathway proteins and associated mRNA

Solubilization and Assay of Cellular and Tissue Protein

Cynthia E. Shaw

Western Immunoblot Analysis

Cynthia E. Shaw and Jing Zheng

Immunohishemistry and Immunocyhemistry

Jing Zheng

Extraction of Cellular and Tissue RNA

Daniel S. Millican and Ian M. Bird

Size Separation and Quantification of mRNA by Northern Analysis

Ian M. Bird

Preparation of Single-Stranded Antisense cDNA Probes by Asymmetric PCR

Daniel S. Millican and Ian M. Bird

Optimization of a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Mass Assay for Low-Abundance mRNA

Jacqueline M. Cale, Cynthia E. Shaw, and Ian M. Bird

Index

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)