Phospholipid Signaling Protocols

Phospholipid Signaling Protocols

by Ian Bird
     
 

This wide-ranging collection of state-of-the-art techniques for the essay of phospholipid-derived second messengers allows the identification and quantification of signal pathway activation. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomylinase and associated metabolites. Additional protocols are provided for the assay of… See more details below

Overview

This wide-ranging collection of state-of-the-art techniques for the essay of phospholipid-derived second messengers allows the identification and quantification of signal pathway activation. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomylinase and associated metabolites. Additional protocols are provided for the assay of phosphoinositide kinase activity and for the separation and identification of phospholipids, diacylglycerol, sphingolipids, and fatty acid metabolites. Detailed, step-by-step instructions make this book suitable for both the newcomer and the experienced investigator.

Editorial Reviews

Booknews
Contributors detail techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites. There is also extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA. Annotation c. by Book News, Inc., Portland, Or.
From the Publisher
". . . the detailed information provided in Phospholipid Signaling Prools makes it a welcome addition to an academic library that serves scientists actively working in the area of second messenger and membrane lipid chemistry. It is well representative of the previous volumes in the Methods in Molecular Biology series, and therefore is a worthwhile acquisition."-E-Streams

". . .this book is an extremely valuable resource for methodology in studying the molecular species of key phospholipid signaling molecules, as well as the enzymes that produce or degrade them. I personally have used several prools in this book, and have not had any trouble adapting the methods to my own applications."-The Quarterly Review of Biology

"...gives an excellent overview of the most common techniques in use as well in biochemistry and molecular biology...The editor is to be congratulated for putting these diverse, very important prools together..."-Cell Biology International 2000

"...does a very good job of covering what is a fairly diverse field and the prools described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signaling molecules."-Molecular Biotechnology

"...does a very good job covering what is a fairly diverse field and the prools described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signalling molecules." - Molecular Biotechnology

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Product Details

ISBN-13:
9781489942609
Publisher:
Springer-Verlag New York, LLC
Publication date:
04/30/2014
Series:
Methods in Molecular Biology Series, #105
Edition description:
Softcover reprint of the original 1st ed. 1998
Pages:
380
Product dimensions:
6.00(w) x 9.00(h) x 0.82(d)

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