Photosynthesis Research Protocols

Photosynthesis Research Protocols

by Robert Carpentier
     
 

ISBN-10: 1588292320

ISBN-13: 9781588292322

Pub. Date: 02/18/2010

Publisher: Springer-Verlag New York, LLC

This book offers a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists. It covers the key techniques for plant bioenergetics as well as those fundamental to plant productivity and biomass, making this an invaluable resource for scientists working on any of the multiple aspects of

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Overview

This book offers a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists. It covers the key techniques for plant bioenergetics as well as those fundamental to plant productivity and biomass, making this an invaluable resource for scientists working on any of the multiple aspects of photosynthesis.

Product Details

ISBN-13:
9781588292322
Publisher:
Springer-Verlag New York, LLC
Publication date:
02/18/2010
Series:
Methods in Molecular Biology Series
Pages:
360
Product dimensions:
9.21(w) x 6.14(h) x 0.81(d)

Table of Contents

1Fractionation of thylakoid membranes into Grana and Stroma Thylakoids1
2Isolation of photosystem I particles from spinach11
3Rapid isolation and purification of photosystem I chlorophyll-binding protein from chlamydomonas reinhardtii19
4Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants29
5Isolation of functional photosystem II core particles from the cyanobacterium Synechocystis sp. PCC 680337
6Isolation of photosystem I reaction center preparation from spinach49
7Isolation of photosystem II reaction center complexes from plants53
8Isolation of photosystem I reaction center subunit polypeptides from spinach63
9Purification and crystallization of the cytochrome b[subscript 6]f complex in oxygenic photosynthesis67
10Purification of plastocyanin and cytochrome c[subscript 6] from plants, green algae, and cyanobacteria79
11Preparation of native and recombinant light-harvesting cholorphyll-a/b complex93
12Isolation and characterization of lamellar aggregates of LHCII and LHCII-lipid macro-assemblies with light-inducible structural transitions105
13Separation, purification, and characterization of polypeptide composition of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II115
14Isolation of CP43 and CP47 photosystem II proximal antenna complexes from plants129
15The determination and quantification of photosynthetic pigments by reverse phase high-performance liquid chromatography, thin-layer chromatography, and spectrophotometry137
16Isolation and identification of chloroplast lipids149
17DNA adducts with chlorophyll and chlorophyllin as antimutagenic agents : synthesis, stability, and structural features159
18Incorporation and analysis of LHCII in model systems173
19Photosystem II reconstitution into proteoliposomes : structure-function characterization183
20Extraction of the functional manganese and calcium from photosystem II205
21Assay of photoinhibition of photosystem II and protease activity217
22Thermoluminescence : a technique for probing photosystem II229
23Detection of free radicals and reactive oxygen species249
24Stabilization of photosynthetic materials261
25Determination of phosphoproteins in higher plant thylakoids271
26Identifying photoprotection mutants in Arabidopsis thaliana287
27A simple method for chloroplast transformation in Chlamydomonas reinhardtii301
28The construction of gene knockouts in the Cyanobacterium Synechocystis sp. PCC 6803309
29Gene inactivation in the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum using in vitro-made DNA constructs and natural transformation325

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