Photosynthesis Research Protocols

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Overview

The capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first edition, Photosynthesis Research Prools, Second Edition presents detailed descriptions of a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists, all of which are contributed by leading researchers in the field. This vital new edition also features coverage of methods related to the most abundant protein on earth, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. Written in the highly successful Methods in Molecular Biology™ series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and expert tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Photosynthesis Research Prools, Second Edition serves new photosynthesis researchers as well as experienced scientists seeking to use a new type of preparation or method to study this tremendously influential process.

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Editorial Reviews

From the Publisher
From the reviews of the second edition:
“This book presents a set of prools focused to the study of photosynthesis from multiple points of view. … new chapters have been added to this edition dealing with the isolation, quantification of Rubisco and Rubisco activase. All the contributors are experienced scientists on the analyzed subjects. The structure of each chapter … enhances the value of the information. In conclusion, this book is a fundamental reference for plant researchers, both for the wide spectrum of subjects covered and the simplicity of the layout.” (Alejandra Landau and Noemí Colombo, Boletin de Bibleoteca del IMYZA-INTA, Issue 26, June, 2011)
“This book represents a collection of materials and methods that are highly useful for researchers in the field of photosynthesis. … various chapters have been revised and the literature has been updated. … every lab working in the field of photosynthesis should have at least one copy. The prools described in the various chapters are written in a concise and understandable way.” (Reimund Goss, Journal of Plant Physiology, Vol. 169, 2012)
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Product Details

  • ISBN-13: 9781588292322
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 2/18/2010
  • Series: Methods in Molecular Biology Series
  • Pages: 360
  • Product dimensions: 9.21 (w) x 6.14 (h) x 0.81 (d)

Table of Contents

1 Fractionation of thylakoid membranes into Grana and Stroma Thylakoids 1
2 Isolation of photosystem I particles from spinach 11
3 Rapid isolation and purification of photosystem I chlorophyll-binding protein from chlamydomonas reinhardtii 19
4 Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants 29
5 Isolation of functional photosystem II core particles from the cyanobacterium Synechocystis sp. PCC 6803 37
6 Isolation of photosystem I reaction center preparation from spinach 49
7 Isolation of photosystem II reaction center complexes from plants 53
8 Isolation of photosystem I reaction center subunit polypeptides from spinach 63
9 Purification and crystallization of the cytochrome b[subscript 6]f complex in oxygenic photosynthesis 67
10 Purification of plastocyanin and cytochrome c[subscript 6] from plants, green algae, and cyanobacteria 79
11 Preparation of native and recombinant light-harvesting cholorphyll-a/b complex 93
12 Isolation and characterization of lamellar aggregates of LHCII and LHCII-lipid macro-assemblies with light-inducible structural transitions 105
13 Separation, purification, and characterization of polypeptide composition of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II 115
14 Isolation of CP43 and CP47 photosystem II proximal antenna complexes from plants 129
15 The determination and quantification of photosynthetic pigments by reverse phase high-performance liquid chromatography, thin-layer chromatography, and spectrophotometry 137
16 Isolation and identification of chloroplast lipids 149
17 DNA adducts with chlorophyll and chlorophyllin as antimutagenic agents : synthesis, stability, and structural features 159
18 Incorporation and analysis of LHCII in model systems 173
19 Photosystem II reconstitution into proteoliposomes : structure-function characterization 183
20 Extraction of the functional manganese and calcium from photosystem II 205
21 Assay of photoinhibition of photosystem II and protease activity 217
22 Thermoluminescence : a technique for probing photosystem II 229
23 Detection of free radicals and reactive oxygen species 249
24 Stabilization of photosynthetic materials 261
25 Determination of phosphoproteins in higher plant thylakoids 271
26 Identifying photoprotection mutants in Arabidopsis thaliana 287
27 A simple method for chloroplast transformation in Chlamydomonas reinhardtii 301
28 The construction of gene knockouts in the Cyanobacterium Synechocystis sp. PCC 6803 309
29 Gene inactivation in the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum using in vitro-made DNA constructs and natural transformation 325
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