Physical Biochemistry: Principles and Applications / Edition 2

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Physical Biochemistry: Principles and Applications provides a broad overview of the key techniques currently used to characterise the structure and function of complex biomacromolecules such as proteins and DNA. It bridges the gap between general biochemistry textbooks and the more specialist texts covering individual techniques." "Topics covered include chromatography, spectroscopy, mass spectrometry, electrophoresis, X-ray diffraction, centrifugation and biocalorimetry. New developments are placed in context by describing the physical principles on which they depend, examining the range of biophysical applications most widely used and, emphasising the overall similarities of experimental approach." "Physical Biochemistry: Principles and Applications will be invaluable to undergraduates and postgraduates studying biochemistry, molecular biology and related disciplines.
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Editorial Reviews

This undergraduate text offers an overview of the techniques used to characterize the structure and function of complex biomacromolecules such as proteins and DNA. Sheehan (biochemistry, University College Cork) covers topics such as chromatography, spectroscopy, electrophoresis, X-ray diffraction, and biocalorimetry, explaining the physical principles upon which these developments depend, and comparing the differences and similarities of these experimental procedures. Annotation c. Book News, Inc., Portland, OR (
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Product Details

  • ISBN-13: 9780470856024
  • Publisher: Wiley
  • Publication date: 5/26/2009
  • Edition description: Revised Edition
  • Edition number: 2
  • Pages: 422
  • Sales rank: 1,296,887
  • Product dimensions: 7.60 (w) x 9.90 (h) x 1.10 (d)

Table of Contents

Ch. 1 Introduction 1
1.1 Special chemical requirements of biomolecules 1
1.2 Factors affecting analyte structure and stability 3
1.3 Buffering systems used in biochemistry 7
1.4 Quantitation, units and data handling 9
1.5 Objectives of this book 10
Ch. 2 Chromatography 12
2.1 Principles of chromatography 12
2.2 Performance parameters used in chromatography 16
2.3 Chromatography equipment 23
2.4 Modes of chromatography 26
2.5 Open-column chromatography 44
2.6 High-performance liquid chromatography 46
2.7 Fast protein liquid chromatography 50
2.8 Perfusion chromatography 51
2.9 Membrane-based chromatography systems 54
2.10 Chromatography of a sample protein 55
Ch. 3 Spectroscopic Techniques 61
3.1 The nature of light 61
3.2 The electromagnetic spectrum 65
3.3 Ultraviolet/visible absorption spectroscopy 66
3.4 Fluorescence spectroscopy 74
3.5 Spectroscopic techniques using plane-polarised light 88
3.6 Infrared spectroscopy 95
3.7 Nuclear magnetic resonance (NMR) spectroscopy 103
3.8 Electron spin resonance (ESR) spectroscopy 109
3.9 Lasers 113
Ch. 4 Mass spectrometry 121
4.1 Principles of mass spectrometry 121
4.2 Mass spectrometry of proteins and peptides 134
4.3 Interfacing MS with other methods 135
4.4 Uses of mass spectrometry in biochemistry 138
Ch. 5 Electrophoresis 153
5.1 Principles of electrophoresis 153
5.2 Non-denaturing electrophoresis 160
5.3 Denaturing electrophoresis 164
5.4 Electrophoresis in DNA sequencing 169
5.5 Isoelectric focusing (IEF) 177
5.6 Two-dimensional SDS page 183
5.7 Immunoelectrophoresis 186
5.8 Agarose gel electro-phoresis of nucleic acids 190
5.9 Pulsed field gel electrophoresis 193
5.10 Capillary electrophoresis 197
5.11 Electroblotting procedures 204
5.12 Electroporation of cells 211
Ch. 6 Three-dimensional structure determination of macromolecules 215
6.1 The protein-folding problem 216
6.2 Structure determination by NMR 230
6.3 Crystallisation of biomacromolecules 245
6.4 X-ray diffraction by crystals 257
6.5 Calculation of electron density maps 263
6.6 Other diffraction methods 279
6.7 Comparison of X-ray crystallography with multi-dimensional NMR 281
6.8 Structural databases 282
Ch. 7 Hydrodynamic methods 287
7.1 Viscosity 287
7.2 Sedimentation 292
7.3 Methods for varying buffer conditions 305
7.4 Flow cytometry 310
Ch. 8 Biocalorimetry 317
8.1 The main thermodynamic parameters 318
8.2 Isothermal titration calorimetry 321
8.3 Differential scanning calorimetry 323
8.4 Determination of thermodynamic parameters by non-calorimetric means 326
App. 1 SI units 329
App. 2 The Fourier transform 330
Index 335
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