The Physiology and Biochemistry of Prokaryotes / Edition 4

Hardcover (Print)
Rent from
(Save 74%)
Est. Return Date: 10/21/2014
Buy New
Buy New from
Used and New from Other Sellers
Used and New from Other Sellers
from $90.93
Usually ships in 1-2 business days
(Save 31%)
Other sellers (Hardcover)
  • All (42) from $90.93   
  • New (22) from $123.89   
  • Used (20) from $90.93   


This important new textbook is the culmination of 25 years of teaching microbial physiology and metabolism to undergraduate and graduate students. A theme that guided the writing of this textbook is that students must not simply memorize material such as metabolic pathways and bioenergetic models, but they should understand the underlying principles. To that end, similarities and contrasts between metabolic pathways and other physiological activities are pointed out through the textbook, and separate sections are devoted to describing the chemistry and physics for key reactions. In addition to describing physiological and metabolic features common to most microorganisms, the textbook also offers a modern perspective on metabolic diversity. Topics included in this text are: cell structure, growth, bioenergetics, electron transport, photosynthesis, metabolic regulation, intermediary metabolism, cell wall biosynthesis, fermentations, C1 metabolism, inorganic metabolism, solute transport, protein secretion, homeostasis, and two-component as well as other signaling systems. Each chapter concludes with a summary emphasizing the major points covered, and a set of study questions to test the students' mastery of the material.
Read More Show Less

Product Details

  • ISBN-13: 9780195393040
  • Publisher: Oxford University Press
  • Publication date: 12/2/2011
  • Edition description: New Edition
  • Edition number: 4
  • Pages: 656
  • Sales rank: 714,726
  • Product dimensions: 6.90 (w) x 10.10 (h) x 1.20 (d)

Meet the Author

David White is Professor Emeritus of Biology at Indiana University. He has taught numerous courses in the areas of microbiology, biochemistry, human biology, and biology.

Read More Show Less

Table of Contents

Chapter 1. Structure and Function
1.1 Phylogeny
1.2 Cell Structure
1.3 Summary
Study Questions
Reference and Notes

Chapter 2. Growth and Cell Division
2.1 Measurement of Growth
2.2 Growth Physiology
2.3 Growth Yields
2.4 Growth Kinetics
2.5 Steady State Growth and Continuous Growth
2.6 Cell Division
2.7 Summary
Study Questions
References and Notes

Chapter 3. Chromosome Replication and Partitioning of Chromosomes
3.1 DNA Replication, Chromosome Separation, and Chromosome Partitioning
3.2 Summary
Study Questions
References and Notes

Chapter 4. Membrane Bioenergetics: The Proton Potential
4.1 The Chemiosmotic Theory
4.2 Electrochemical Energy
4.3 The Contributions of the Δψ and the ΔpH to the Overall Δp in Neutrophiles, Acidophiles, and Alkaliphiles
4.4 Ionophores
4.5 Measurement of the δp
4.6 Use of the δp To Do Work
4.7 Exergonic Reactions That Generate a δp
4.8 Other Mechanisms For Creating a Δψ or a δp
4.9 Halorhodopsin, a Light-Driven Chloride Pump
4.10 The δp and ATP Synthesis in Alkaliphiles
4.11 Summary
Study Questions
References and Notes

Chapter 5. Electron Transport
5.1 Aerobic and Anaerobic Respiration
5.2 The Electron Carriers
5.3 Organization of the Electron Carriers in Mitochondria
5.4 Organization of The Electron Carriers in Bacteria
5.5 Coupling Sites
5.6 How a Proton Potential Might Be Created at the Coupling Sites: Q Loops, Q Cycles, and Proton Pumps
5.7 Patterns of Electron Flow in Individual Bacterial Species
5.8 Summary
Study Questions
References and Notes

Chapter 6. Photosynthesis
6.1 The Phototrophic Prokaryotes
6.2 The Purple Photosynthetic Bacteria
6.3 The Green Sulfur Bacteria (Chlorobiaceae)
6.4 Cyanobacteria and Chloroplasts
6.5 Efficiency of Photosynthesis
6.6 Photosynthetic Pigments
6.7 The Transfer of Energy from the Light Harvesting Pigments to the Reaction Center
6.8 The Structure of Photosynthetic Membranes in Bacteria
6.9 Summary
Study Questions
References and Notes

Chapter 7. The Regulation of Metabolic Pathways
7.1 Patterns of Regulation of Metabolic Pathways
7.2 Kinetics of Regulatory and Nonregulatory Enzymes
7.3 Conformational Changes in Regulatory Enzymes
7.4 Regulation by Covalent Modification
7.5 Summary
Study Questions
References and Notes

Chapter 8. Bioenergetics in the Cytosol
8.1 High-Energy Molecules and Group Transfer Potential
8.2 The Central Role of Group Transfer Reactions in Biosynthesis
8.3 ATP Synthesis by Substrate Level Phosphorylation
8.4 Summary
Study Questions
References and Notes

Chapter 9. Central Metabolic Pathways
9.1 Glycolysis
9.2 The Fate of NADH
9.3 Why Write NAD+ Instead of NAD, and NADH Instead of NADH2?
9.4 A Modified EMP Pathway in the Hyperthermophilic Archaeon Pyrococcus furiosus
9.5 The Pentose Phosphate Pathway
9.6 The Entner-Doudoroff Pathway
9.7 The Oxidation of Pyruvate to Acetyl-CoA: The Pyruvate Dehydrogenase Reaction
9.8 The Citric Acid Cycle
9.9 Carboxylations that Replenish Oxaloacetate: The Pyruvate and Phosphoenolpyruvate Carboxylases
9.10 Modification of the Citric Acid Cycle Into a Reductive (Incomplete) Cycle During Fermentative Growth
9.11 Chemistry of Some of the Reactions in the Citric Acid Cycle
9.12 The Glyoxylate Cycle
9.13 Formation of Phosphoenolpyruvate
9.14 Formation of Pyruvate from Malate
9.15. Summary of the Relationships Between the Pathways
9.16 Summary
Study Questions
References and Notes

Chapter 10. Metabolism of Lipids, Nucleotides, Amino Acids, and Hydrocarbons
10.1 Lipids
10.2 Nucleotides
10.3 Amino Acids
10.4 Aliphatic Hydrocarbons
10.5 Summary
Study Questions
References and Notes

Chapter 11. RNA and Protein Synthesis
11.1 RNA Synthesis
11.2 Protein Synthesis

Chapter 12. Cell Wall and Capsule Biosynthesis
12.1 Peptidoglycan
12.2 Lipopolysaccharide
12.3 Extracellular Polysaccharide Synthesis and Export in Gram Negative Bacteria
12.4 Levan and Dextran Synthesis
12.5 Glycogen Synthesis
12.6 Summary
Study Questions
References and Notes

Chapter 13. Inorganic Metabolism
13.1 Assimilation of Nitrate and Sulfate
13.2 Dissimilation of Nitraate and Sulfate
13.3 Nitrogen Fixation
13.4 Lithotrophy
13.5 Summary
Study Questions
Reference and Notes

Chapter 14. C1 Metabolism
14.1 Carbon Dioxide Fixation Systems
14.2 Growth on C1 Compounds Other than CO2: The Methylotrophs
14.3 Summary
Study Questions
References and Notes

Chapter 15. Fermentations
15.1 Oxygen Toxicity
15.2 Energy Conservation by Anaerobic Bacteria
15.3 Electron Sinks
15.4 The Anaerobic Food Chain
15.5 How to Balance a Fermentation
15.6 Propionate Fermentation via the Acrylate Pathway
15.7 Propionate Fermentation via the Succinate-Propionate Pathway
15.8 Acetate Fermentation ( Acetogenesis)
15.9 Lactate Fermentation
15.10 Mixed-Acid and Butanediol Fermentation
15.11 Butyrate Fermentation
15.12 Ruminococcus albus
15.13 Summary
Study Questions
References and Notes

Chapter 16. Responses to Environmental Stress
16.1 Maintaining a δpH
16.2 Osmotic Pressure and Osmotic Potential
16.3 Heat-Shock Response (HSR)
16.4 Repairing Damaged DNA
16.5 The SOS Response
16.6 Oxidative Stress
16.7 Summary
Study Questions
References and Notes

Chapter 17. Solute Transport
17.1 The Use of Proteoliposomes to Study Solute Transport
17.2 Kinetics of Solute Uptake
17.3 Energy-Dependent Transport
17.4 How to Determine the Source of Energy for Transport
17.5 Drug-Export Systems
17.6 Bacterial Transport Systems in Summary
17.7 Summary
Study Questions
References and Notes

Chapter 18. Protein Transport and Secretion
18.1 The Sec System
18.2 The Translocation of Membrane-Bound Proteins
18.3 The E. coli SRP
18.4 Protein Translocation of Folded Proteins: The TAT System
18.5 Extracellular Protein Secretion
18.6 Folding of Periplasmic Proteins
18.7 Summary
Study Questions
References and Notes

Chapter 19. Responses to Environmental Cues
19.1 Introduction to Two-Component Signaling Systems
19.2 Responses by Facultative Anaerobes to Anaerobiosis
19.3 Response to Nitrate and Nitrite: The Nar Regulatory System
19.4 Response to Nitrogen Supply: The Ntr Regulon
19.5 Response to Inorganic Phosphate Supply: The Pho Regulon
19.6 Effect of Oxygen and Light on the Expression of Photosynthetic Genes in the Purple Photosynthetic Bacterium Rhodobacter capsulatus
19.7 Response to Osmotic Pressure and Temperature: Regulation of Porin Synthesis
19.8 Response to Potassium Ion and External Osmolarity: Stimulation of Transcription of the kdpABC Operson by a Two-Component Regulatory System
19.9 Acetyl Phosphate Is a Possible Global Signal in Certain Two-Component Systems
19.10. Response to Carbon Sources: Catabolite Repression, Inducer Expulsion, Permease Synthesis
19.11. Virulence Factors: Synthesis in Response to Temperature, pH, Nutrient Osmolarity, and Quorum Sensors
19.12. Summary
Study Questions
References and Notes

Chapter 20. Chemotaxis, Photoresponses, Aerotaxis
20.1 Bacteria Measure Changes in Concentration Over Time
20.2 Tumbling
20.3 Adaptation
20.4 Proteins Required for Chemotaxis
20.5 A Model for Chemotaxis
20.6 Mechanism of Repellent Action
20.7 Chemotaxis That Does Not Use MCPs: The Phosphotransferase System Is Involved In Chemotaxis Toward PTS Sugars
20.8 Chemotaxis That Is Not Identical With The Model Proposed For The Enteric Bacteria
20.9 Photoresponses
20.10 Halobacteria
20.11 Photosynthetic Bacteria
20.12 Aerotaxis
20.13 Summary
Study Questions
References and Notes

Chapter 21. Microbial Biofilms - Structured Multicellular Assemblies
21.1 Bacterial Multicellular Structures
21.2 Prevalence and Importance of Biofilms
21.3 Properties of Biofilms
21.4 Progression of Biofilm Formation and Dissolution
21.5 Regulation of Biofilm Formation
21.6 Inhibition of Biofilm Formation
21.7 Evolutionary Processes in Biofilms
21.8 Summary
Study Questions
References and Notes

Chapter 22. Cell-Cell Communication Mechanisms
22.1 Diversity of Diffusible Signal Molecules Produced by Bacteria
22.2 Specific Signaling Systems
22.3 Cell-Cell Signaling that Requires Contact
22.4 Summary
Study Questions
References and Notes

Chapter 23. Bacterial Development
23.1 Myxobacteria
23.2 Caulobacter
23.3 Sporulation in Bacillus subtilis
23.4 Summary
Study Questions
References and Notes

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)