Plant Cytogenetics

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Plant cytogenetics has progressed at a rapid rate since the publication of the first edition. Plant Cytogenetics, Second Edition presents an up-to-date review of the subject that reflects this progress. It covers the latest in the various classical and modern techniques in the handling of chromosomes, karyotype analysis, genetics of meiosis, genomic relationships, and chromosome manipulation. It includes new chapters on extra chromosomal inheritance and the mode of reproduction in plants, particularly apomixis, ...
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second Good [ No Hassle 30 Day Returns ] [ Edition: second ] Publisher: CRC Press Pub Date: 5/25/1993 Binding: Hardcover Pages: 416.

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Overview

Plant cytogenetics has progressed at a rapid rate since the publication of the first edition. Plant Cytogenetics, Second Edition presents an up-to-date review of the subject that reflects this progress. It covers the latest in the various classical and modern techniques in the handling of chromosomes, karyotype analysis, genetics of meiosis, genomic relationships, and chromosome manipulation. It includes new chapters on extra chromosomal inheritance and the mode of reproduction in plants, particularly apomixis, as well as new sections on the molecular basis of heredity, genomic in situ hybridization, and the classical and molecular methods of genome analysis. The author also elaborates on the cytogenetic basis of somaclonal variation generated through cell and tissue culture.

Providing a bridge between the classical and the current, this second edition of a bestseller presents an up-to-date review of cytogenetics. It covers the latest in the various techniques in the handling of chromosomes, karyotype analysis, genetics of meiosis, genomic relationships, and chromosome manipulation. It includes new chapters on extra chromosomal inheritance and the mode of reproduction in plants as well as new sections on the molecular basis of heredity, genomic in situ hybridization, and the molecular method of genome analysis. The author also elaborates on the cytogenetic basis of somaclonal variation generated through cell and tissue culture.

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Product Details

  • ISBN-13: 9780849386565
  • Publisher: CRC Press
  • Publication date: 5/25/1993
  • Pages: 416
  • Product dimensions: 6.48 (w) x 9.61 (h) x 0.95 (d)

Table of Contents

Ch. 1 Introduction 1
Ch. 2 The Handling of Plant Chromosomes 7
Squash Technique - Mitotic and Meiotic Chromosomes 7
Smear Technique for Plant Chromosomes 18
Pollen Staining 21
Pollen-Stigma Incompatibility 24
Fluorescence in Situ Hybridization (FISH) 26
Total DNA Extraction - Plant Genomic DNA 35
Determination of Nuclear DNA Content of Plants by Flow Cytometry 38
Karyotyping and Sorting of Plant Chromosomes by Flow Cytometry 48
Production of Wide Hybrids through in Vitro Technique 57
Ch. 3 Cell Division 59
Mitosis 59
Meiosis 65
Ch. 4 Genetic Control of Meiosis 73
Synaptic Mutants 73
Genetic Control of Recombination 83
Genes Responsible for Chromosome Disjunction 84
Other Meiotic Mutants 85
Role of Heterochromatin in Chromosome Pairing 86
Diploid-Like Meiosis in Allopolyploids 86
Haploidy 88
Male Sterility 90
Ch. 5 Mode of Reproduction in Plants 93
Sexual Reproduction 93
Asexual Reproduction 94
The Chromosomal Basis of Sex Determination in Plants 106
Ch. 6 Karyotype Analysis 113
Nomenclature of Chromosomes 113
Karyotype Analysis by Mitotic Metaphase Chromosomes 116
Karyotype Analysis by Pachytene Chromosomes 120
Karyotype Analysis by Flow Cytometry 120
Karyotype Analysis by Image Analysis 123
Chromosome Number of Economically Important Plants 123
Ch. 7A Chromosomal Aberrations - Structural Chromosome Changes 125
Deficiencies 125
Duplications 134
Interchanges 137
Inversions 150
Ch. 7B Chromosomal Aberrations - Numerical Chromosome Changes 157
Euploidy 157
Aneuploidy: Trisomics 173
Monosomics and Nullisomics 234
Alien Addition Lines 249
Alien Substitution Lines 263
Ch. 8 Genome Analysis 277
Classical Taxonomy and Genome Designations 277
Genomic Relationships among Diploid Species 279
Genomes of Glycine 287
Genomes of Gossypium 290
Ancestors of Allopolyploid Species 292
Genome Analysis by Molecular Methods 303
Ch. 9 Chromosomal Aberrations in Cell and Tissue Culture 307
Chromosomal Aberrations in Callus 307
Mechanism of Chromosomal Aberrations in Culture 314
Chromosomal Aberrations in Regenerated Plants 316
Chromosomal Aberrations in Haploid Callus and Their Regenerants 321
Chromosomal Aberrations in Somatic Hybrids 322
Ch. 10 Transgenic Crops 327
Present Status of Transgenic Crops 327
Production of Transgenic Crops 327
Chromosomal Aberrations in Transgenic Crops 329
Cytological Basis of Gene Silencing 334
App. 2-I: Sources of Chemicals 337
App. 2-II Materials for Flow Cytometry 339
App. 2-III Reagents and Solutions 341
App. 2-IV Preparation of Media 347
App. 6-I Common Name, Scientific Name, and 2n Chromosome Number of Some Important Plants 349
App. 7-I Linkage Map of Barley 353
Glossary 359
References 385
Index 443
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