Plant Cytogenetics, Second Edition / Edition 2

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Overview

Plant cytogenetics has progressed at a rapid rate since the publication of the first edition. Plant Cytogenetics, Second Edition presents an up-to-date review of cytogenetics. It covers the latest in the various classical and modern techniques in the handling of chromosomes, karyotype analysis, genetics of meiosis, genomic relationships, and chromosome manipulation. It includes new chapters on extra chromosomal inheritance and the mode of reproduction in plants, paricularly apomixis, as well as new sections on the molecular basis of heredity, genomic in situ hybridization, and the classical and molecular methods of genome analysis. The author also elaborates on the cytogenetic basis of somaclonal variation generated through cell and tissue culture.

Providing a bridge between the classical and the current, this second edition of a bestseller presents an up-to-date review of cytogenetics. It covers the latest in the various techniques in the handling of chromosomes, karyotype analysis, genetics of meiosis, genomic relationships, and chromosome manipulation. It includes new chapters on extra chromosomal inheritance and the mode of reproduction in plants as well as new sections on the molecular basis of heredity, genomic in situ hybridization, and the molecular method of genome analysis. The author also elaborates on the cytogenetic basis of somaclonal variation generated through cell and tissue culture.

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Editorial Reviews

From the Publisher
Singh's book Plant Cytogenetics is a timely review of the advances in the field. ... the book includes an informative appendix about reagents, solutions and media, and a thorough glossary. Plant Cytogenetics is a remarkable book and a broad array of readers will find the entire book, or particular sections, of interest. The conceptual organization emphasizes basic themes in cytogenetics, combined with examples of information published in leading journals in the field. The text would provide students and other researchers an excellent basis for development of new hypothesis in chromosomal aberrations and the selection of plant cytogenetic techniques. ... Singh's work exemplifies the maturation of cytogenetics and the deep analysis of techniques involved in research. This book provides an excellent entry level introduction for those wishing to augment their understanding in the field. Any researcher in botany would do well to consult the relevant sections of Singh's book, both for his thoughtful treatments of many topics, and for the numerous case studies reported.
—Claudia Segovia-Salcedo, University of Florida, Gainesville, USA, in Economic Botany, 2009, Vol. 63, No. 1

"Plant Cytogenetics" by Ram Singh gives an outstanding treatment of classical cytogenetics and then ably integrates molecular cytogenetic approaches. While commercial crops have been studied in the greatest detail cytogenetically, the species are treated not in specialized chapters but under cytogenetic principles. A mastery of the cytogenetic phenomena that has been reported for these crops, such as structural and numerical chromosome analyses (Chapter 7) and genome analysis with examples from Glycine, Gossypium, Brassica, Nicotiana and Triticum (Chapter 8) enable one to be completely familiar with the mechanisms of cytogenetics. Many Tables will be found especially useful, such as the list of primary trisomics derived from autotriploids, and the alien chromosome substitution list giving plant recipient, donor, procedure for transfer and genes transferred. … There is an excellent Glossary of 25 pages and a list of References comprising 58 pages. An extensive Index serves both as a subject matter and a species index. The text is quite adequately illustrated with 114 figures. …"Plant Cytogenetics" is a solid text embracing both classical and molecular aspects of cytogenetics. The Chapter on cytogenetic techniques will make the text a classic in its own right. In conclusion, I highly recommend the text for a thorough understanding of the basic mechanisms of plant cytogenetics for which the text is primarily designed.
—William F. Grant, Department of Plant Science, McGill University, Quebec, Canada

This is a solid text…I recommend the text for a thorough understanding of the basic mechanisms of plant cytogenetics for which the text is primarily designed. ... This is a comprehensive handbook that deals both with the theoretical and practical aspects related to plant cytogenetics and will be a valuable reference work for those entering the field for the first time, those who occasionally venture into the field, plant breeders, and those involved in active research.
—Glendon Ascough, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, in South African Journal of Botany, 2007, Vol. 73, No. 3

Praise for the First Edition:

For advanced students who are looking for descriptions of the mechanics of the wide variety of chromosomal aberrations that have been studied in crop plants, this text should be of considerable use.
Plant Science Bulletin

… provides an excellent review of various techniques in handling of chromosomes, karyotype analysis, genetics of meiosis, genomic relationships, and chromosome manipulations. In addition to his own studies, Dr. Singh has made extensive use of the information published in various journals…The contents of the book are well arranged and easy to read and understand. It should serve as a useful textbook for students taking courses in genetics and cytogenetics and as a reference book for scientists engaged in plant cytogenetic research.
Theoretical and Applied Genetics

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Product Details

  • ISBN-13: 9780849323881
  • Publisher: CRC Press
  • Publication date: 11/1/1998
  • Edition description: Revised
  • Edition number: 2
  • Pages: 488
  • Product dimensions: 7.00 (w) x 10.00 (h) x 1.06 (d)

Table of Contents

INTRODUCTION

THE HANDLING OF PLANT CHROMOSOMES
Introduction
Squash Technique - Mitotic and Meiotic Chromosomes
Smear Technique for Plant Chromosomes
Pollen Staining
Pollen-Stigma Incompatibility
Fluorescence in situ Hybridization (FISH)
Total DNA Extraction-Plant Genomic DNA
Determination of Nuclear DNA Content of Plants by Flow Cytometry
Karyotyping and Sorting of Plant Chromosomes by Flow Cytometry
Production of Wide Hybrids Through In Vitro Technique

CELL DIVISION
Introduction
Mitosis
Meiosis

GENETIC CONTROL OF MEIOSIS
Introduction
Synaptic Mutants
Genetic Control of Recombination
Genes Responsible for Chromosome Disjunction
Other Meiotic Mutants
Role of Heterochromatin in Chromosome Pairing
Diploid-like Meiosis in Allopolyploids
Haploidy
Male Sterility

MODE OF REPRODUCTION IN PLANTS
Introduction
Sexual Reproduction
Asexual Reproduction
The Chromosomal Basis of Sex Determination in Plants

KARYOTYPE ANALYSIS
Introduction
Nomenclature of Chromosomes
Karyotype Analysis by Mitotic Metaphase Chromosomes
Karyotype Analysis by Pachytene Chromosomes
Karyotype Analysis by Flow Cytometry
Karyotype Analysis by Image Analysis
Chromosome Number of Economically Important Plants

CHROMOSOMAL ABERRATIONS - STRUCTURAL CHROMOSOME CHANGES
Deficiencies
Duplications
Interchanges
Inversions

CHROMOSOMAL ABERRATIONS - NUMERICAL CHROMOSOME CHANGES
Euploidy
Aneuploidy: Trisomics
Monosomics and Nullisomics
Alien Addition Lines
Alien Substitution Lines

GENOME ANALYSIS
Introduction
Classical Taxonomy and Genome Designations
Genome Relationships among Diploid Species
Genomes of Glycine
Genomes of Gossypium
Ancestors of Allopolyploid Species
Genome Analysis by Molecular Methods

CHROMOSOMAL ABERRATIONS IN CELL AND TISSUE CULTURE
Introduction
Chromosomal Aberrations in Callus
Mechanism of Chromosomal Aberrations in Culture
Chromosomal Aberrations in Regenerated Plants
Chromosomal Aberrations in Haploid Callus and Their Regenerants
Chromosomal Aberrations in Somatic Hybrids

TRANSGENIC CROPS
Introduction
Present Status of Transgenic Crops
Production of Transgenic Crops
Chromosome Aberrations in Transgenic Crops
Cytological Basis of Gene Silencing

APPENDICES

REFERENCES

GLOSSARY

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