Plant Metabolic Flux Analysis: Methods and Protocols

by Martine Dieuaide-Noubhani (Editor), Ana Paula Alonso (Editor)

Overview

In Plant Metabolic Flux Analysis, expert researchers in the field provide detailed experimental procedures for each step of the flux quantification workflow. Steady state and dynamic modeling are considered, as well as recent developments for the reconstruction of metabolic networks and for a predictive modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical Plant Metabolic Flux Analysis, seeks to aid scientists in the further study of cutting-edge prools and methodologies that are crucial to getting ahead in MFA.

Product Details

ISBN-13:	9781627036870
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/13/2013
Series:	Methods in Molecular Biology , #1090
Edition description:	2014
Pages:	365
Product dimensions:	7.01(w) x 10.00(h) x 0.04(d)

Table of Contents

Application of Metabolic Flux Analysis to Plants.- Metabolic Network Reconstruction and Their Topological Analysis.- ¹⁴C pulse Labeling to Estimate External Fluxes and Turnovers in Primary Metabolism.- Optimization of Steady-state ¹³C-labeling Experiments for Metabolic Flux Analysis.- Quantification of ¹³C Enrichments and Isotopomer Abundances for Metabolic Flux analysis using 1D NMR Spectroscopy.- Analysis of Proteinogenic Amino Acid and Starch Labeling by 2D NMR.- Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS.- Quantifying ¹³C-labeling in Free Sugars and Starch by GC-MS.- Liquid Chromatography Tandem Mass Spectrometry for Measuring ¹³C-labeling in Intermediates of the Glycolysis and Pentose-phosphate Pathway.- In Vivo NMR for ¹³C metabolic Flux Analysis.- Steady State and Instationary Modeling of Proteinogenic and Free Amino Acid Isotopomers for Flux Quantification.- Isotopically Nonstationary MFA (INST-MFA) of Autotrophic Metabolism.- Simulating Labeling to Estimate Kinetic Parameters for Flux Control Analysis.- High-throughput Data Pipelines for Metabolic Flux Analysis in Plants.- Analysis of Enzyme Activities.- Analytical Kinetic Modeling: A Practical Procedure.- Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling.- Flux Variability Analysis: Application to Developing Oilseed Rape Embryos using Toolboxes for Constraint-Based Modeling.- Plant Genome-Scale Modeling and Implementation.- ³⁴S and ¹⁵N Labelling to Model S and N Flux in Plants and Determine the Different Components of N and S use Efficiency.- Ecophysiological Process-based Model to Simulate Carbon Fluxes in Plants.