Plant Pathology: Techniques and Protocols / Edition 1

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Plant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid. Chapters examine recent and developing issues with crop identity and authenticity, allowing workers to genotype samples from two major food groups. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and reader-friendly, Plant Pathology: Techniques and Protocols is an incredible guide which will soon prove to be indispensable, both to novices and expert researchers alike.
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Editorial Reviews

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"Plant Pathology: Techniques and Prools provides a collection of methods for the diagnosis and detection of plant pathogens. … the book can also be used to troubleshoot particular techniques and adapt it to any organism of interest. The notes at the end of each chapter are of great utility … . It is a highly practical book and both students and researchers interested or working on detection and diagnosis will find it very useful." (Arantza Rico, Microbiology Today, November, 2009)

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Product Details

  • ISBN-13: 9781588297990
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/9/2008
  • Series: Methods in Molecular Biology Series, #508
  • Edition description: 2009
  • Edition number: 1
  • Pages: 326
  • Product dimensions: 7.50 (w) x 10.30 (h) x 0.80 (d)

Table of Contents

Preface v

Contributors ix

1 Bioassay for Diagnosis of Plant Viruses Isla A. Browning 1

2 Development of Enzyme Linked, Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection Hei-ti Hsu 15

3 Immunisation Strategies for Antibody Production Robert Burns 27

4 Preparation of Immunogens and Production of Antibodies Hei-ti Hsu Tsung-chi Chen Chin-an Chang Shyi-dong Yeh 37

5 Enzyme-Linked Immunosorbent Assay Detection of Ralstonia solanacearum in Potatoes: The South African Experience Dirk U. Bellstedt 51

6 Production of Monoclonal Antibodies to Plant Pathogens Christopher R. Thornton 63

7 Enzyme-Linked Immunosorbent Assay for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus) Blanka Kokoskova Jaap D. Janse 75

8 Indirect Immunofluorescence Microscopy for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Ralstonia solanacearum) Jaap D. Janse Blanka Kokoskova 89

9 Detection for Four Major Bacterial Potato Pathogens Ellen M. Kerr Greig Cahill Karen Fraser 101

10 Erwinia amylovora: Modern Methods for Detection and Differentiation Antonet M. Svircev Won-Sik Kim Susan M. Lehman Alan J. Castle 115

11 The Use of Fluorescent In Situ Hybridization in Plant Fungal Identification and Genotyping Mohamed Hijri 131

12 Use of Molecular Methods for the Detection of Fungal Spores Elaine Ward 147

13 Identification of Phytophthora fragariae var. rubi by PCR Alexandra Schlenzig 161

14 Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani Nikki D. Charlton Stellos M. Tavantzis Marc A. Cubeta 171

15Immunocapture-PCR for Plant Virus Detection Vincent Mulholland 183

16 Multiplex Polymerase Chain Reaction (PCR) and Real-Time Multiplex PCR for the Simultaneous Detection of Plant Viruses V. Pallas J. Sanchez-Navarro A. Varga F. Aparicio D. James 193

17 Fluorescent-Based Techniques for Viral Detection, Quantification, and Characterization Mathieu Rolland Agnes Delaunay Emmanuel Jacquot 209

18 Analysis of Population Structures of Viral Isolates Using Single-Strand Conformation Polymorphism Method Agnes Delaunay Mathieu Rolland Emmanuel Jacquot 235

19 Direct Detection of Plant Viruses in Potato Tubers Using Real-Time PCR Neil Boonham Lynn Laurenson Rebecca Weekes Rick Mumford 249

20 An Accelerated Soil Bait Assay for the Detection of Potato Mop Top Virus in Agricultural Soil Triona Davey 259

21 Detection of Phytoplasmas of Temperate Fruit Trees Margit Laimer 267

22 PCR Detection of Potato Cyst Mematode Alex Reid 289

23 Potato Cultivar Genome Analysis Alex Reid Lysbeth Hof Danny Esselink Ben Vosman 295

24 Barley Variety Identification Using SSRs Cathy Southworth 309

Index 319

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