Plant Proteomics: Methods and Protocols / Edition 1

Hardcover (Print)
Buy New
Buy New from
Used and New from Other Sellers
Used and New from Other Sellers
from $39.00
Usually ships in 1-2 business days
(Save 80%)
Other sellers (Hardcover)
  • All (11) from $39.00   
  • New (8) from $66.96   
  • Used (3) from $39.00   


Plant Proteomics: Methods and Prools provides up-to-date methods and prools for plant proteomics, each written by expert researchers from leading laboratories. Experimental methodologies are explained in clear and complete detail for both the wet and dry laboratories. The methods and prools presented in this volume will inspire the novice as well as the expert researcher to undertake new experimental endeavors in plant proteomics.

Chapters 1 and 2 describe standard prools and chapters 3 through 10 contain optimized prools for protein extraction of tissues such as cereal seeds, xylem, phloem sap, of woody plants, and of cellular compartments, such as chloroplasts and mihondria. These methods rely on improvements to solubilization of proteins, which is the emphasis of Chapters 11 and 12. Chapters 13 to 23 outline methods for protein identification, including 2DLC or LC of intact proteins, mass spectrometry (MALDI-TOF and MS/MS) with proteins separated by 2D gels, blue native ID gels, and Edman sequencing. Chapters 24 to 29 focus on the rapidly expanding area of analysis of post-translational modifications and protein-protein relationships. Attention is also given to data that proteomics produces, including management of that data, and to statistical tools used for effective analysis.

Read More Show Less

Product Details

  • ISBN-13: 9781588296351
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 9/22/2006
  • Series: Methods in Molecular Biology Series, #355
  • Edition description: 2007
  • Edition number: 1
  • Pages: 416
  • Product dimensions: 9.00 (w) x 6.00 (h) x 1.06 (d)

Table of Contents

Total Protein Extraction with TCA-Acetone
Valérie Méchin, Catherine Damerval and Michel Zivy

Phenol Extraction of Proteins for Proteomic Studies of Recalcitrant Plant Tissues
Mireille Faurobert, Esther Pelpoir and Jamila Chaïb

Protein Extraction from Cereal Seeds
Gérard Branlard and Emmanuelle Bancel

Protein Extraction from Xylem and Phloem Sap
Julia Kehr and Martijn Rep

Protein Extraction from Woody Plants
Christophe Plomion and Céline Lalanne

Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis
Klaas J. van Wijk, Jean-Benoit Peltier and Lisa Giacomelli

Isolation and Sub-Fractionation of Plant Mihondria for Proteomic Analysis
Holger Eubel, Joshua L. Heazlewood and A. Harvey Millar

Extraction of Nuclear Proteins from Root Meristematic Cells
Fernando González-Camacho and Francisco Javier Medina

Isolation of Nuclear Proteins
Setsuko Komatsu

Isolation of Cell Wall Proteins from Medicago sativa Stems
Bonnie S. Watson and Lloyd W. Sumner

Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis
Véronique Santoni

Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis
Thierry Rabilloud, Sylvie Luche, Véronique Santoni and Mireille Chevallet

Two-Dimensional Electrophoresis for Plant Proteomics
Walter Weiss and Angelika Görg

Visible and Fluorescent Staining of Two-Dimensional Gels
François Chevalier, Valérie Rofidal and Michel Rossignol

Two-Dimensional Differential In-Gel Electrophoresis (DIGE) of Leaf and Roots of Lycopersicon esculentum
Matthew Keeler, Jessica Letarte, Emily Hattrup, Fatimah Hickman and Paul A. Haynes

Quantitative Analysis of 2D Gels
Michel Zivy

Multivariate Data Analysis of Proteome Data
KåreEngkilde, Susanne Jacobsen and Ib Søndergaard

Edman Sequencing of Plant Proteins from 2D Gels
Setsuko Komatsu

Peptide Mass Fingerprinting, Identification of Proteins by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF)
Nicolas Sommerer, Delphine Centeno and Michel Rossignol

Protein Identification using Nano Liquid Chromatography: Tandem Mass Spectrometry
Luc Negroni

Two-Dimensional Nanoflow Liquid Chromatography: Tandem Mass Spectrometry of Proteins Extracted from Rice Leaves and Roots
Linda Breci and Paul A. Haynes

Separation, Identification and Profiling of Membrane Proteins by GFC/ IEC/SDS-PAGE and MALDI TOF MS
Wojciech Szponarski, Frédéric Delom, Nicolas Sommerer, Michel Rossignol and Rémy Gibrat

The PROTICdb Database for 2-DE Proteomics
Olivier Langella, Michel Zivy and Johann Joets

Identification of Phosphorylated Proteins
Maria V. Turkina and Alexander V. Vener

Plant Proteomics and Glycosylation
Anne-Catherine Fitchette, Olivia Tran Dinh, Loïc Faye and Muriel Bardor

Blue-Native Gel Electrophoresis for the Characterization of Protein Complexes in Plants
Jesco Heinemeyer, Dagmar Lewejohann and Hans-Peter Braun

Electroelution of Intact Proteins from SDS-PAGE Gels and Their Subsequent MALDI-TOF MS Analysis
Zhentian Lei, Ajith Anand, Kirankumar S. Mysore and Lloyd W. Sumner

Generation of Plant Protein Microarrays and Investigation of Antigen-Antibody Interactions
Birgit Kersten and Tanja Feilner

Phosphorylation Studies Using Plant Protein Microarrays
Tanja Feilner and Birgit Kersten

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)