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Plant Proteomics: Methods and Protocols / Edition 1

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Overview

Plant Proteomics: Methods and Prools provides up-to-date methods and prools for plant proteomics, each written by expert researchers from leading laboratories. Experimental methodologies are explained in clear and complete detail for both the wet and dry laboratories. The methods and prools presented in this volume will inspire the novice as well as the expert researcher to undertake new experimental endeavors in plant proteomics.

Chapters 1 and 2 describe standard prools and chapters 3 through 10 contain optimized prools for protein extraction of tissues such as cereal seeds, xylem, phloem sap, of woody plants, and of cellular compartments, such as chloroplasts and mihondria. These methods rely on improvements to solubilization of proteins, which is the emphasis of Chapters 11 and 12. Chapters 13 to 23 outline methods for protein identification, including 2DLC or LC of intact proteins, mass spectrometry (MALDI-TOF and MS/MS) with proteins separated by 2D gels, blue native ID gels, and Edman sequencing. Chapters 24 to 29 focus on the rapidly expanding area of analysis of post-translational modifications and protein-protein relationships. Attention is also given to data that proteomics produces, including management of that data, and to statistical tools used for effective analysis.

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Product Details

  • ISBN-13: 9781588296351
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 9/22/2006
  • Series: Methods in Molecular Biology Series , #355
  • Edition description: 2007
  • Edition number: 1
  • Pages: 416
  • Product dimensions: 9.00 (w) x 6.00 (h) x 1.06 (d)

Table of Contents

Total Protein Extraction with TCA-Acetone
Valérie Méchin, Catherine Damerval and Michel Zivy

Phenol Extraction of Proteins for Proteomic Studies of Recalcitrant Plant Tissues
Mireille Faurobert, Esther Pelpoir and Jamila Chaïb

Protein Extraction from Cereal Seeds
Gérard Branlard and Emmanuelle Bancel

Protein Extraction from Xylem and Phloem Sap
Julia Kehr and Martijn Rep

Protein Extraction from Woody Plants
Christophe Plomion and Céline Lalanne

Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis
Klaas J. van Wijk, Jean-Benoit Peltier and Lisa Giacomelli

Isolation and Sub-Fractionation of Plant Mihondria for Proteomic Analysis
Holger Eubel, Joshua L. Heazlewood and A. Harvey Millar

Extraction of Nuclear Proteins from Root Meristematic Cells
Fernando González-Camacho and Francisco Javier Medina

Isolation of Nuclear Proteins
Setsuko Komatsu

Isolation of Cell Wall Proteins from Medicago sativa Stems
Bonnie S. Watson and Lloyd W. Sumner

Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis
Véronique Santoni

Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis
Thierry Rabilloud, Sylvie Luche, Véronique Santoni and Mireille Chevallet

Two-Dimensional Electrophoresis for Plant Proteomics
Walter Weiss and Angelika Görg

Visible and Fluorescent Staining of Two-Dimensional Gels
François Chevalier, Valérie Rofidal and Michel Rossignol

Two-Dimensional Differential In-Gel Electrophoresis (DIGE) of Leaf and Roots of Lycopersicon esculentum
Matthew Keeler, Jessica Letarte, Emily Hattrup, Fatimah Hickman and Paul A. Haynes

Quantitative Analysis of 2D Gels
Michel Zivy

Multivariate Data Analysis of Proteome Data
KåreEngkilde, Susanne Jacobsen and Ib Søndergaard

Edman Sequencing of Plant Proteins from 2D Gels
Setsuko Komatsu

Peptide Mass Fingerprinting, Identification of Proteins by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF)
Nicolas Sommerer, Delphine Centeno and Michel Rossignol

Protein Identification using Nano Liquid Chromatography: Tandem Mass Spectrometry
Luc Negroni

Two-Dimensional Nanoflow Liquid Chromatography: Tandem Mass Spectrometry of Proteins Extracted from Rice Leaves and Roots
Linda Breci and Paul A. Haynes

Separation, Identification and Profiling of Membrane Proteins by GFC/ IEC/SDS-PAGE and MALDI TOF MS
Wojciech Szponarski, Frédéric Delom, Nicolas Sommerer, Michel Rossignol and Rémy Gibrat

The PROTICdb Database for 2-DE Proteomics
Olivier Langella, Michel Zivy and Johann Joets

Identification of Phosphorylated Proteins
Maria V. Turkina and Alexander V. Vener

Plant Proteomics and Glycosylation
Anne-Catherine Fitchette, Olivia Tran Dinh, Loïc Faye and Muriel Bardor

Blue-Native Gel Electrophoresis for the Characterization of Protein Complexes in Plants
Jesco Heinemeyer, Dagmar Lewejohann and Hans-Peter Braun

Electroelution of Intact Proteins from SDS-PAGE Gels and Their Subsequent MALDI-TOF MS Analysis
Zhentian Lei, Ajith Anand, Kirankumar S. Mysore and Lloyd W. Sumner

Generation of Plant Protein Microarrays and Investigation of Antigen-Antibody Interactions
Birgit Kersten and Tanja Feilner

Phosphorylation Studies Using Plant Protein Microarrays
Tanja Feilner and Birgit Kersten

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