Plant Tissue Culture Concepts and Laboratory Exercises / Edition 2

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Alternating between topic discussions and hands-on laboratory experime nts that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Seco nd Edition, addresses the most current principles and methods in plant tissue culture research.

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Editorial Reviews

A text/lab manual suitable for both beginning and advanced students introducing plant tissue culture history and complete laboratory exercises for a wide variety of agronomic and horticulturally important plants. The 39 chapters, written by biologists and plant specialists from universities across the country, dissect methodologies and techniques in propagation and crop improvement with step-by step instructions and, in general terms, the results of each experiment. The laboratory projects use such diverse plants as the chrysanthemum, ajuga, watermelon, peanut, and lilac. Each topic area also includes a special discussion section to stimulate creativity and problem solving. Plastic comb binding. Annotation c. Book News, Inc., Portland, OR (
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Product Details

  • ISBN-13: 9780849320293
  • Publisher: Taylor & Francis
  • Publication date: 11/28/1999
  • Edition description: New Edition
  • Edition number: 2
  • Pages: 472
  • Product dimensions: 7.70 (w) x 10.00 (h) x 1.00 (d)

Table of Contents

(Note: Topic chapters appear below in bold upper and lower case letters; Laboratory chapters appear below in nonbold upper and lower case letters.)
Introduction of plant tissue and cell culture
History of plant tissue and cell culture
Getting started with tissue culture-Media preparation, sterile technique and laboratory equipment
Nutrition of callus cultures
Histological techniques
Microscopy and photography
Statistical analysis of plant tissue culture data
Propagation from preexisting meristems
Micropropagation of Syngonium by shoot culture
Micropropagation of Dieffenbachia
Micropropagation of potato by node culture and microtuber production
Micropropagation of lilac
Micropropagation and in vitro flowering of rose
Propagation from nonmeristematic tissues- organogenesis
Direct shoot organogenesis from leaf explants of chrysanthemum
Shoot organogenesis of watermelon
Direct and indirect shoot organogenesis from leaves of Torenia fournieri
Shoot organogenesis from petunia leaves
Propagation from nonmeristematic tissues- Nonzygotic embryogenesis
Embryogenic callus and suspension cultures from leaves of orchardgrass
Somatic embryogenesis from seeds of melon
Somatic embryogenesis from mature peanut seed
Direct somatic embryogenesis from leaves and flower receptacles of cineraria
Somatic embryogenesis from immature seeds of yellow poplar
Introduction of somatic embryogenesis in conifers
Use of protoplasts for plant improvement
Isolation, culture and fusion of tobacco and potato protoplasts
Isolation of protoplasts from leaves of chrysanthemum and orchardgrass
Haploid cultures
Production of haploid tobacco plants using another culture
Transformation of tobacco and carrot using Agrobacterium tumefaciens and expression of the ß-glucuronidase (GUS) reporter gene
Transformation of chrysanthemum leaf explants using Agrobacterium tumefaciens
Construction and use of a simple gene gun for particle bombardment
Germplasm preservation
Vitrification as a method to cryopreserve shoot tips
Secondary product expression in vitro
Pigment production in Ajuga cell culture
In vitro plant pathology
Variation in culture
Tissue culture of ferns
Commercial laboratory production
Indexing for plant pathogens
Culture indexing for bacterial and fungal contaminants

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