BN.com Gift Guide

Plant Transposable Elements: Methods and Protocols

Overview

Transposable elements have played a major role in shaping plant genome structure and gene expression. Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome. In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient ...

See more details below
Other sellers (Hardcover)
  • All (10) from $61.85   
  • New (8) from $70.88   
  • Used (2) from $61.85   
Sending request ...

Overview

Transposable elements have played a major role in shaping plant genome structure and gene expression. Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome. In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Transposable Elements: Methods and Protocols seeks to aid scientists in the further study of transposons by providing essential background information and specific experimental protocols.

Read More Show Less

Product Details

  • ISBN-13: 9781627035675
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 7/31/2013
  • Series: Methods in Molecular Biology Series , #1057
  • Edition description: 2013
  • Edition number: 1
  • Pages: 324
  • Product dimensions: 7.10 (w) x 10.10 (h) x 1.00 (d)

Table of Contents

1. Historical Overview of Transposable Element Research

Peter A Peterson

2. Distinguishing Variable Phenotypes from Variegation Caused by Transposon Activities

Virginia Walbot

3. Using Transposons for Genetic Mosaic Analysis of Plant Development

Philip W. Becraft

4. Survey of Natural and Transgenic Gene Markers Used to Monitor Transposon Activity

Lakshminarasimhan Krishnaswamy, Thomas Peterson

5. Molecular Biology of Maize Ac/Ds Elements – an Overview

Katina Lazarow, My-Linh Du and Reinhard Kunze

6. Gene Tagging with Engineered Ds Elements in Maize

Yubin Li, Gregorio Segal, Qinghua Wang, and Hugo K. Dooner

7. Plant Regeneration Methods for Rapid Generation of a Large Scale of Ds Transposant Population in Rice

Yuan Hu Xuan, Jin Huang, Gihwan Yi, Dong-Soo Park, Soo Kwon Park, Moo Young Eun, Doh Won Yun, Gang-Seob Lee, Tae Ho Kim, Chang-deok Han

8. Isolation of Sequences Flanking Ac Insertion Sites by Ac Casting

Dafang Wang and Thomas Peterson

9. Regulation of the Mutator System of Transposons in Maize

Damon Lisch

10. Using MuDR/Mu Transposons in Directed Tagging Strategies

Virginia Walbot and Julia Qüesta

11. Genetic and Molecular Analysis of UniformMu Transposon Insertion Lines

Donald R. McCarty, Masaharu Suzuki, Charles Hunter, Wayne T. Avigne, and Karen E. Koch

12. Digestion-Ligation-Amplification (DLA): A Simple Genome Walking Method to Amplify Unknown Sequences Flanking Mutator (Mu) Transposons and Thereby Facilitate Gene Cloning

Sanzhen Liu, An-Ping Hsia, Patrick S. Schnable

13. Molecular Genetics and Epigenetics of CACTA Elements

Nina V. Fedoroff

14. Activation Tagging Using the Maize En-I Transposon System for the Identification of Abiotic Stress Resistance Genes in Arabidopsis

Amal Harb and Andy Pereira

15. Reverse Genetics in Rice using Tos17

Delphine Mieulet, Anne Diévart, Gaëtan Droc, Nadège Lanau and Emmanuel Guiderdoni

16. Identification and Applications of the Petunia Class II Act1/dTph1 Transposable Element System

Tom Gerats , Jan Zethof, and Michiel Vandenbussche

17. Transposon Display: A Versatile Method for Transposon Tagging

Michiel Vandenbussche, Jan Zethof, and Tom Gerats

18. Massive Indexed Parallel Identification of Transposon Flanking Sequences

Michiel Vandenbussche, Jan Zethof, and Tom Gerats

19. Use of Next Generation Sequencing (NGS) Technologies for the Genome-wide Detection of Transposition

Moaine Elbaidouri, Cristian Chaparro and Olivier Panaud

20. Overview of Repeat Annotation and de novo Repeat Identification

Ning Jiang

21. Computational Methods for Identification of DNA Transposons

Ning Jiang

22. TEnest 2.0: Computational Annotation and Visualization of Nested Transposable Elements

Brent A. Kronmiller and Roger P. Wise

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)