Practical Flow Cytometry / Edition 4

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This complete reference provides the background science and discussion of the vast biomedical applications of quantitative analytical cytology using laser-activated detection and cell sorting. Now in its fourth edition, this text has been expanded to provide full coverage of the broad spectrum of applications in molecular biology and biotechnology today. New to this edition are chapters on automated analysis of array technologies, compensation, high-speed sorting, reporter molecules, and multiplex and apoptosis assays, along with fully updated and revised references and a list of suppliers.

The book contains black-and-white illustrations.

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Editorial Reviews

From the Publisher
"one of the most valuable of its genre and...addressed to a wide audience.... Written in such an attractive way, being both informative and stimulating" (Zbigniew Darzynkiewicz, Trends in Cell Biology)

"...there is no question that this is THE reference book on flow cytometry...You need this book if you use flow cytometry. Get it!" (Doody's Electronic Journal, October 25, 2003)

"...This complete reference examines the vast biomedical applications-fully updated and revised..." (Jnl. Of Investigational Allergology and Clinical Immunology, vol 13; no 4)

Doody's Review Service
Reviewer: Valerie L. Ng, PhD MD(Alameda County Medical Center/Highland Hospital)
Description: This is a truly comprehensive book on everything you could ever want to know about flow cytometry.
Purpose: The purpose of this book is to be THE reference book on flow cytometry. This is the fourth edition of this book, and there is no question that it is THE reference book on flow cytometry!
Audience: This book would be useful to anyone who uses flow cytometry or relies on this technology for their research or clinical care.
Features: This book continues in its grand tradition of being the "bible" of flow cytometry. I cannot adequately describe its depth and breadth, so I resort to sheer astounding numbers to portray its comprehensiveness - 566 pages of 10 point font, surprisingly few illustrations (yes, it's primarily text), 3,685(!) references. In briefly skimming the book, I was overwhelmed by the massive amount of information. No question that I'll have this book within easy reach whenever I field questions about flow cytometry! A really nice touch in this book is the author's chatty (and sometimes quirky) writing style. The reader will not only find accurate and complete factual information, but will also appreciate flow cytometry-themed lyrics to sing to the tune for famous melodies, Shapiro's various (and amusing) laws, and helpful tips (including the final one that warns the user to never use a staff to smite a clogged cytometer). The author's humor and conversational style makes interesting reading and the information incredibly accessible. A major difference between this and the third edition is the elimination of the section on the "do-it-yourself" flow cytometer (e.g., "cytomutt") - the author's acknowledgement of the widespread penetration of commercial systems in the field. In this regard, there is a very helpful chapter listing the various manufacturers and companies from which reagents can be purchased. Finally - and rare in these types of comprehensive books - there is touching recognition to those who were pioneers in this field. A really nice human touch to a very complex subject.
Assessment: Wow! You need this book if you use flow cytometry. Get it!
T. Vincent Shankey
This is a comprehensive treatment of flow cytometry and the diverse technologies that are used to prepare and analyze samples for analysis using flow cytometry. The information provided is useful to understand how to effectively use flow cytometry, including what went wrong and how to fix it, how to analyze the data generated, and how to improve the whole lot. The purpose is to ""include almost anything anyone might want, or need to know about flow cytometry."" With the continuing improvements to the technologies and increasing areas of application of flow cytometry, the current revision (currently in its third edition) represents a timely and needed expansion of earlier editions. This book is targeted at anyone who uses or wishes to use a flow cytometer. Although the treatment of all areas begins with fundamental principles, current areas of application and new technologies and applications are included, making the book of interest to beginners and experts alike. The presentation is uniform and the book is easy to read. Although the diversity of subject places significant demands on a single author, Dr. Shapiro is unique in this field in his comprehension of all these areas. The book includes a wealth of practical information, including a listing other books published on any aspect of flow cytometry, societies involved with this technology, and a listing of instrument and reagent sources. This book is an encyclopedia of flow cytometry. The only criticism might be that it covers every aspect of flow cytometry in considerable detail, sometimes more detail than a reader pushed by many demands wants (including why the flow cytometry experiment did not work). Most flow cytometrylaboratories already have previous editions placed near the cytometer, and the more sophisticated or advanced the laboratory, the more battered and used the book. With the improvements and expansion in this edition, it will likely sit next to previous and well-worn editions in most flow cytometry laboratories (including mine).

4 Stars! from Doody
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Product Details

  • ISBN-13: 9780471411253
  • Publisher: Wiley
  • Publication date: 12/6/2002
  • Edition description: REV
  • Edition number: 4
  • Pages: 736
  • Sales rank: 1,528,603
  • Product dimensions: 8.66 (w) x 11.06 (h) x 1.76 (d)

Table of Contents

List of Tables and Figures.

Preface to the Fourth Edition: Why You Should Read the Book – Or Not.

Foreword to the Third Edition.

Preface to the Third Edition.

Preface to the Second Edition.

Foreword to the First Edition.

Preface to the First Edition.

1. Overture.

2. Learning Flow Cytometry.

3. History.

4. How Flow Cytometers Work.

5. Data Analysis.

6. Flow Sorting.

7. Parameters and Probes.

8. Buying Flow Cytometers.

9. Building Flow Cytometers.

10. Using Flow Cytometers: Applications, Extensions, and Alternatives.

11. Sources of Supply.

12. Afterword.

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